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51.
In mammalian cells, base pairing between the U2 and U6 small nuclear RNAs is required during pre-RNA splicing. We show by psoralen crosslinking of HeLa nuclear extract that U2.U6 base pairing occurs within abundant ribonucleoprotein complexes that sediment at > 150 S in glycerol gradients. All of the spliceosomal RNAs (U1, U2, U4, U5, and U6) cosediment with these large complexes, suggesting that they may be related to small nuclear RNA-containing structures called speckles/coiled bodies or snurposomes, which have been visualized in mammalian or amphibian nuclei, respectively. In contrast to nuclear extract, S100 extract, which is splicing-defective and lacks the > 150S complexes, does not contain base-paired U2.U6. However, U2.U6 base pairs form in S100 extract that has been made splicing-competent by supplementation with Ser/Arg-rich (SR) proteins, ATP, and an adenovirus splicing substrate. During splicing in supplemented S100 extract, U2.U6 base pairing precedes the appearance of splicing intermediates and occurs initially in an approximately 60S spliceosome complex but also in progressively larger (100-300 S) complexes. Possible functional relationships between the 60S spliceosome and the > 150S complexes are discussed. 相似文献
52.
TheSpecial Issue on Applications of Temporal Models raises many issues of time: What are the important properties of time? How can time be best represented? How can one reason about time-dependent properties? What are the important directions of temporal research? This introductory piece very briefly surveys the current wide variety of temporal models, temporal reasoning methods, and applications to time-varying phenomena. Promising areas of investigation such as the verification of concurrent systems, knowledge-base representation methods, and dealing with theFrame Problem pass in fleeting review. Brief introductions to each of the works in the volume close the section. 相似文献
53.
Extending the Kernighan/Lin Heuristic for Hardware and Software Functional Partitioning 总被引:5,自引:0,他引:5
The Kernighan/Lin graph partitioning heuristic, also known as min-cut or group migration, has been extended over several decades very successfully for circuit partitioning. Those extensions customized the heuristic and its associated data structure to rapidly compute the minimum-cut metric central to circuit partitioning; as such, those extensions are not directly applicable to other problems. In this paper, we extend the heuristic for functional partitioning, which in turn can solve the much investigated codesign problem of partitioning a system's coarse-grained functions among hardware and software components. The key extension customizes the heuristic and data structure to rapidly compute execution-time and communication metrics, crucial to hardware and software partitioning, and leads to near-linear time-complexity and excellent resulting quality. Another extension uses a new criteria for terminating the heuristic, eliminating time-consuming and unnecessary fine-tuning of a partition. Our experiments demonstrate extremely fast execution times (just a few seconds) with results matched only by the slower simulated annealing heuristic, meaning that the extended Kernighan/Lin heuristic will likely prove hard to beat for hardware and software functional partitioning. 相似文献
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Judiesch Michael K.; Schmidt Frank L.; Mount Michael K. 《Canadian Metallurgical Quarterly》1992,77(3):234
Examined distributions of estimates of the dollar value of performance in studies employing the method of F. L. Schmidt et al (1979) for estimating the standard deviation of job performance (SDy) and found evidence that (1) the mean 50th percentile estimate is biased downward, (2) estimates of SDy appear to be a constant percentage of the 50th percentile estimate, and (3) estimates of SDy as a percentage of the 50th percentile value (SDp) are quite similar to empirical SDp values based on actual employee output. These findings suggest that the downward bias in the mean estimate of the 50th percentile causes the mean estimates of SDy to be similarly biased downward, but does not bias the estimates of SDp. Finally, an objective method for estimating the value of average employee output is described. The product of this value and the mean supervisory estimate of SDp yields an unbiased estimate of SDy. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
58.
DA Gray 《Canadian Metallurgical Quarterly》1997,54(6):1553-1562
This study demonstrates that invertebrate acoustic signals can provide information about male phenotypic attributes, and that females can use this acoustic information in mate choice to select a phenotypically superior mate. I investigated the relationships between a male acoustic sexual signal, the phenotype of the signaller, and the female response to signal variation. I recorded and analysed the calling songs of male house crickets, Acheta domesticus. The analyses showed that chirps convey information about male size. With the exception of amplitude, the mean number of pulses per chirp was the best predictor of male size. I performed a laboratory tape-playback experiment to determine female preference during phonotaxis. Females preferred tapes playing the chirps of large males, specifically chirps with a greater number of pulses per chirp. Selection on the female preference is discussed.Copyright 1997 The Association for the Study of Animal Behaviour 相似文献
59.
DA Bloch 《Canadian Metallurgical Quarterly》1997,53(1):73-85
Methods are proposed for comparing two diagnostic tests for the same data where a threshold for positive for each test is specified. One method contrasts the diagnostic tests' estimated risks. A second method compares the two tests' kappa coefficients. When thresholds for positive test results are specified a priori, maximum likelihood estimators and their asymptotic variances are derived and test statistics are presented for both case-control and naturalistic methods of sampling. The bootstrap is proposed as a method to assess differences in risk estimators when thresholds for positive test results are chosen by scanning the data. Examples are given to illustrate the methods. 相似文献
60.
We have measured the heat capacities of3He films and liquid3He in porous Vycor glass at 10 to 600 mK. With increasing the film thickness from 1 to 3 atomic layers, the specific heat evolves gradually from that typical to solid to that of liquid3He. At about 2 atomic layers, however, its low-temperature part is nearly temperature-independent; we interpret this as a result of gradual freezing of spins in an amorphous solid3He film with decreasing the temperature. The contribution of liquid3He in the center of the Vycor pores can be described as the specific heat of bulk liquid3He at corresponding pressures in the range 0 to 28 bar. The thickness of amorphous solid on the pore walls increases with external pressure roughly linearly. Preplating the walls with4He allows to determine the positions of3He atoms contributing to the surface specific heat at 10 to 50 mK. In addition, the contribution from the specific heat of3 He -4He mixing at 100 to 600 mK is discussed as a function of pressure and amount of4He.0n leave from ISSP Acad. Sci. of Russia, Chernogolovka, Russia 相似文献