Steroid-responsive encephalomyelitis in childhood

The syndrome of parainfectious encephalomyelitis evolves from an antecedent infection. Several etiologic agents have been associated with this complication, although the pathogenesis in each instance may prove to be more uniform. Considerable evidence suggests that the syndrome is mediated immunologically. The seven cases reported here were clinically similar, although the infectious etiologies were diverse. Leptospirosis antedated the neurologic syndrome in two cases, and a "viral" illness preceded the other five cases. The evolution of the syndrome was slowly progressive in each case, and six patients had prominent involvement of rhombencephalic structures. The progressive course was reversed rapidly with eventual full recovery in each instance after initiation of corticosteroid therapy. Our experience with these cases coupled with a review of the literature suggests that corticosteroid therapy should be considered in the subacute or chronic cases of parainfectious encephalomyelitis.     

A new method for evaluating antiarrhythmic drug efficacy

To develop standards for distinguishing antiarrhythmic drug effect from spontaneous variability of premature ventricular complexes (PVCs), 21 males (mean age 56 +/- 8 years) with chronic ischemic heart disease and PVCs underwent symptom-limited treadmill exercise testing and 24-hour ambulatory monitoring before and after 2 weeks of placebo medication. Linear regression analysis was used to describe the relationship between baseline and placebo PVC frequency for various indexes of ventricular ectopic activity and to establish 95% and 99% one-tailed confidence intervals for this relationship within the group of 21 patients. The lower limit of baseline PVC frequency for which the procedure could distinguish a placebo from a true drug response, termed the "sensitivity threshold," was an average frequency of 2.2 PVCs/hour for ambulatory electrocardiographic monitoring and 1.2 PVCs/min for treadmill exercise testing. All patients exceeded the sensitivity threshold on baseline ambulatory ECGs, but only 38% of patients did so on baseline treadmill exercise tests. To establish antiarrhythmic efficacy with 95% confidence, the minimal percent reduction of PVCs between baseline and placebo visits was 68% for treadmill exercise testing and 65% for ambulatory electrocardiography. Although these standards were developed in patients with chronic ischemic heart disease, the model can be used to establish antiarrhythmic drug efficacy in any patient group.     

Effect of prolactin suppression on gonadotrophic secretion in the puerperium

Serum levels of prolactin (PRL), FSH, LH and oestradiol-17 beta were determined by radioimmunoassay in 57 lactating women and in 20 women in whom lactation was inhibited by ergocryptine (CS-154). Women who breast fed their infants exhibited high PRL levels which abruptly declined within 48 h post-partum, and remained low for the duration of the study. Serum FSH was undetectable during the first week post-partum in lactating as well as in CB-154 treated women. Thereafter, lactating women showed increasing FSH levels which reached a maximum by the third week post-partum. These FSH values were higher in lactating women than in the CBS-154 treated group. In contrast, LH levels were higher in those women receiving CB-154. Serum oestradiol-17 beta remained in low levels throughout the study, and no difference was observed between the two groups of subjects. From these results it seems that: 1) inhibition of PRL secretion leads to a faster recovery of gonadotrophin secretion toward the "menstrual type", and 2) PRL suppression produces no effect on the ovarian oestrogen production.     

Grafting of enzymes on collagen films using Woodward''s reagent "K" and a water-soluble carbodiimide derivative

Two new methods of activation were developed to graft enzymes on collegen films. They involved chemical modifications of surface groups of collagen either by Woodward's reagent "K" or by EDC, a water-soluble derivative of carbodiimide. EDC was a better coupling agent and a detailed study was conducted with this agent. It could be used either in a global method of activation and coupling, or in a two-step procedure of activation of collagen, followed by spontaneous coupling of enzyme. All enzymes tested were successfully bound: malate dehydrogenase, lactate dehydrogenase, aspartate aminotransferase, urease, creatine kinase, hexokinase. The influence on the yield of grafted enzyme, of pretreatment of films, time and temperature of EDC activation, concentration of EDC and enzyme, protecting agents was studied. Stability of enzyme activity on storage was greatly increased after grafting. A co-grafted dual system creatine kinase/heoxkinase, was achieved which exhibited a good efficiency. A striking renaturing process at 0-4degreesC after thermal denaturation, was observed with hexokinase.     

Relationships Between Structure and Activity in the α-Amylase Family of Starch-metabolising Enzymes

α-Amylases are known to be multidomain proteins, i.e., the molecules consist of several folding units. Each α-amylase is believed, however, to have a catalytic domain consisting, of a barrel of eight parallel α-strands surrounded by eight α-strands. with an extra helix inserted after the sixth γ-strands. The α-strands and helices alternate along the polypeptide chain and are linked together by irregular loops. Amino acid residues situated on the loops joining the C-terminal end of each α-strand to the N-terminal end of the following helix make up the active site of the enzymes. A similar structure has been found in cyclodextrin glucanotransfcrases and it is now believed that such a (α/α)8-barrel also constitutes the catalytic domain of enzymes active on α-1.6-glucosidic bonds, and of enzymes with dual specificity for both α-1.4- and α-1.6- bonds. Knowledge of the three-dimensional structure of α-amylases and cyclodextrin glucanotransferase has made possible identification of structural features important for enzymic activity and specificity. By analogy, some general conclusions are reached concerning pullulanase, isoamylase. oligo-1,6-glucosidase, neopullulanase and branching enzymes.     

大英博物馆世界保护与展示中心

世界保护与展示中心将提供世界顶级的设施,以确保大英博物馆更好的为后代保管和研究藏品。[The WCEC]will provide world class facilities that will allow the British Museum to ensure the care and understanding of its collections for future generations. 背景BACKGROUND世界保护与展览中心（WCEC）是大英博物馆260年来最大的再开发项目。该中心位于布鲁姆斯伯里地块的西北角,它将设置新的主展厅、最先进的实验室和工作室、世界顶级的藏品库以及博物馆,为英国乃至全球提供藏品租赁的设施。设计面临的挑战是：既要体现时代特征和大英博物馆的国际水准,又要达到其作为一级保护建筑在遗产保护方面更为宽泛的制度和建筑要求。     

Fourth graders' sensitivity to morphological context in spelling.

An important aspect of spelling development in English involves the child's ability to use morphological context to deal with one-to-many sound-spelling mappings for word endings. One case involves /z/ endings that follow long vowels, where an “s” spelling is required in the case of inflected noun and verb contexts, but an “se” or “ze” spelling is required in the case of noninflected nouns and verbs. The present study examined the ability of good and poor spellers in Grade 4 to capture these morphological distinctions in a pseudoword spelling task. Overall, the good spellers outperformed the poor spellers, and both groups were more sensitive to the inflected–noninflected noun distinction than the inflected–noninflected verb distinction. These findings underscore the importance of linguistic factors in spelling development as well as the necessity of providing due consideration to these factors in spelling instruction. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Calibrating the UHF technique of partial discharge detection usinga PD simulator

The paper describes a technique for simulating the presence of partial discharges in gas-insulated substation (GIS) equipment. The technique employs a pulse generator to inject current pulses through a suitable protrusion. The current pulses have similar temporal characteristics to those produced by an actual discharge event, with the added advantage that their magnitudes can be controlled accurately. Because the pulses are highly reproducible, they are ideally suited for investigating the UHF technique of partial discharge detection. The paper discusses the relationship between the magnitude of the UHF signal received by a coupler and the discharge magnitude. It also shows that the coupler signal is dependent on the protrusion length and on the spatial relationship between the protrusion and the coupler. The implications for practical discharge measurements in GIS also are discussed