Wear and biological activity of highly crosslinked polyethylene in the hip under low serum protein concentrations

Crosslinked ultra-high molecular weight polyethylene (UHMWPE) has been developed and introduced into clinical practice in order to reduce wear in the hip. Zero wear of highly crosslinked UHMWPE in vitro has been reported by some groups using lubricants with high concentrations of serum proteins in hip simulators. In contrast, some clinical studies have reported finite wear rates. The aim of this study was to compare the wear rates, wear surfaces, and wear debris produced by UHMWPE with different levels of crosslinking in a hip joint simulator, with lower, more physiologically relevant concentrations of protein in the lubricant. The UHMWPEs were tested in the Leeds ProSim hip joint simulator against cobalt-chromium (CoCr) femoral heads. The wear particles were isolated and imaged using a field emission gun scanning electron microscope (FEGSEM) at high resolution. The highly crosslinked UHMWPEs had significantly lower wear volumes than the non-crosslinked UHMWPEs. No significant difference was found in the percentage number and percentage volume of the particles in different size ranges from any of the materials. They had similar values of specific biological activity. The functional biological activity (FBA), which takes into account the wear volume and specific biological activity, showed that the highly crosslinked UHMWPEs had lower FBAs due to their lower wear volume.     

Healthcare data warehousing and quality assurance

Healthcare data warehousing presents unique challenges. The industry is rife with often incompatible medical standards and coding schemes that require careful translation. Healthcare data comes from many sources and is delivered in many forms, including published books, individual spreadsheets, and several tape or data formats. Results derived from a healthcare data warehouse must be delivered in accessible form to diverse stakeholders, including healthcare regulators, physicians, hospital administrators, consumers, community activists, and members of the popular press. The industry's widely decentralized and largely autonomous data collection efforts make data quality a significant challenge. Finally, the sensitivity of healthcare data makes privacy and security issues paramount. Healthcare data warehousing will make rigorous, quantitative information available to healthcare decision makers. The authors describe a fully functional healthcare data warehouse used to produce several reports for communities throughout Florida. Building on this work, they're actively pursuing a research agenda to enhance technical data warehousing capabilities while investigating innovative community and clinical healthcare applications     

Model checking agent programming languages

In this paper we describe a verification system for multi-agent programs. This is the first comprehensive approach to the verification of programs developed using programming languages based on the BDI (belief-desire-intention) model of agency. In particular, we have developed a specific layer of abstraction, sitting between the underlying verification system and the agent programming language, that maps the semantics of agent programs into the relevant model-checking framework. Crucially, this abstraction layer is both flexible and extensible; not only can a variety of different agent programming languages be implemented and verified, but even heterogeneous multi-agent programs can be captured semantically. In addition to describing this layer, and the semantic mapping inherent within it, we describe how the underlying model-checker is driven and how agent properties are checked. We also present several examples showing how the system can be used. As this is the first system of its kind, it is relatively slow, so we also indicate further work that needs to be tackled to improve performance.     

The removal of arsenic from （ground）-water by adsorbent loaded in polymeric microspheres

Arsenic is a natural tasteless and odourless element,existing in the earth’s crust at average levels of between two and five thousands micrograms per liter (parts per million) . Arsenic is highly toxic to humans, who are exposed to it primarily from air,food and water. The occurrence of arsenic in groundwater is due to geological composition of soil. High concentrations of arsenic in water are the result of dissolution or desorption of ferric oxides and the oxidation of mineral arsenopyrites. Arsenic in drinking water has an important impact on the human health,especially in the less developed countries. Different methods exist to remove arsenic from aquatic media,and one of them is by adsorption. In this work,the adsorption of both As(III) and As(V) by means of novel microspheres has been investigated. In particular,TiO2 has been embedded into polymeric microspheres PES (PolyEtherSulphone) and PEEK-WC (PolyEtherEther-Ketone) . The main advantages of this encapsulation adsorption material are: no loss of adsorbents into the water stream,easy to be used and scaled-up.     

Transmission microscopy of the corrosion scale formed on a stainless steel exposed to liquid sodium

The scale, formed on 304 stainless steel during exposure to sodium for 85 days, was removed electrolytically, examined in transmission in the Hitachi high voltage microscope and found to consist of hexagonal close packed crystallites, typically 0.3 to 1 m in diameter. Approximately 90% of the crystallites were of basal (0001) orientation and the others either of {4, ¯1, ¯3, 15} or {¯2110} orientations. Lattice parametersa=2.96 Å andc=13.89 Å were determined by analysing electron diffraction patterns derived from the scale. Confirmatory evidence was obtained by examining the scale formed after a shorter exposure of 29 days, in the Philips EM 200 microscope. Previous studies [1, 2] have indicated that the scale may be sodium chromite (with lattice parametersa=2.96 Å andc=15.95 Å). The results of the present study are in apparent conflict with this suggestion unless a contraction parallel to thec-axis 13% is assumed.     

Haemoglobins of the shark, Heterodontus portusjacksoni II. Amino acid sequence of the alpha-chain

The amino acid sequence of the alpha-chain of the principal haemoglobin from the shark, H. portusjacksoni has been determined. The chain has 148 residues and is acetylated at the amino terminal. The soluble peptides obtained by tryptic and chymotryptic digestion of the protein or its cyanogen bromide fragments were isolated by gel filtration, paper ionophoresis and paper chromatography. The amino acid sequences were determined by the dansyl-Edman procedure. The insoluble "core" peptide from the tryptic digestion contained 34 residues and required cleavage by several prosteases before the sequence was established. Compared with human alpha-chain there are 88 amino acid differences including the additional seven residues which appear on the amino terminal of the shark chain. There is also one deletion and one insertion. The chain contains no tryptophan but has four cysteinyl residues which is the highest number of such residues recorded for a vertebrate globin. In the alpha1beta1 contact sites there are four changes in the oxyhaemoglobin form and six deoxy form. Nine of the 16, alpha1beta1 contact sites show variation while three of the haem contact sites have changed in comparison to the residues known to be involved in these interactions in horse haemoglobin alpha-chain. Use of the sequence data to estimate a time of divergence of the shark from the main vertebrate line yielded the value of 410 +/- 46 million years. The data, in general, support the palaeontological view that bony fishes arose before the elasmobranchs.     

Turbulence generated by vibrating wire resonators in superfluid 4He at low temperatures

No Heading We have measured responses of vibrating wire resonators in superfluid ⁴He at millikelvin temperatures. We find evidence for turbulence generation above a critical velocity on the order of a few cm/s. At the critical velocity for the onset of turbulence, the resonator velocity abruptly decreases and shows hysteretic behavior. Surprisingly we find that the resonant frequencies of the resonators increase in the turbulent regime. We also find that the critical velocity may be reduced by the presence of turbulence generated by a neighboring vibrating wire resonator, allowing the detection of existing turbulence in the low temperature regime.PACS numbers: 67.40 Vs, 67.40 Pm     

Model checking rational agents

Agent-oriented programming techniques seem appropriate for developing systems that operate in complex, dynamic, and unpredictable environments. We aim to address this requirement by developing model-checking techniques for the (automatic or semiautomatic) verification of rational-agent systems written in a logic-based agent-oriented programming language. Typically, developers apply model-checking techniques to abstract models of a system rather than the system implementation. Although this is important for detecting design errors at an early stage, developers might still introduce errors during coding. In contrast, developers can directly apply our model-checking techniques to systems implemented in an agent-oriented programming language, automatically verifying agent systems without the usual gap between design and implementation. We developed our techniques for AgentSpeak, a rational-agent programming language based on the AgentSpeak (L) abstract agent-oriented programming language. AgentSpeak shares many features of the agent-oriented programming paradigm. Similarly, we've developed techniques for automatically translating AgentSpeak programs into the model specification language of existing model-checking systems. In this way, we reduce the problem of verifying that an AgentSpeak system has certain BDI logic properties to a conventional LTL model-checking problem.     

Design of a reconfigurable planar parallel manipulator

This work presents the design of a reconfigurable planar parallel manipulator (RPPM). The RPPM is designed to act as a testbed manipulator for theories on redundant actuation of parallel manipulators and can reconfigure into three different revolute-jointed mechanism types: a 2-branch 2-DOF (degree-of-freedom) 5-bar mechanism; a 2-branch 3-DOF 6-bar mechanism; and a 3-branch 3-DOF 8-bar mechanism. The design of the RPPM allows for any shoulder or elbow joint to be actuated. In this work, the criteria and constraints of the design are presented. The final design of the RPPM is shown, followed by a discussion of the final design and how it relates to the initial design criteria and constraints. © 2004 Wiley Periodicals, Inc.     

The effect of glycosaminoglycan depletion on the friction and deformation of articular cartilage

Glycosaminoglycans (GAGs) have been shown to be responsible for the interstitial fluid pressurization of articular cartilage and hence its compressive stiffness and load-bearing properties. Contradictory evidence has been presented in the literature on the effect of depleting GAGs on the friction properties of articular cartilage. The aim of this study was to investigate the effect of depleting GAGs on the friction and deformation characteristics of articular cartilage under different tribological conditions. A pin-on-plate machine was utilized to measure the coefficient of friction of native and chondroitinase ABC (CaseABC)-treated articular cartilage under two different models: static (4 mm/s start-up velocity) and dynamic (4 mm/s sliding velocity; 4 mm stroke length) under a load of 25 N (0.4 MPa contact stress) and with phosphate-buffered saline as the lubricant. Indentation tests were carried out at 1 N and 2 N loads (0.14 MPa and 0.28 MPa contact stress levels) to study the deformation characteristics of both native and GAG-depleted cartilage samples. CaseABC treatment rendered the cartilage tissue soft owing to the loss of compressive stiffness and a sulphated-sugar assay confirmed the loss of GAGs from the cartilage samples. CaseABC treatment significantly increased (by more than 50 per cent) the friction levels in the dynamic model (p < 0.05) at higher loading times owing to the loss of biphasic lubrication. CaseABC treatment had no effect on friction in the static model in which the cartilage surfaces did not have an opportunity to recover fluid because of static loading unlike the cartilage tissue in the dynamic model, in which translation of the cartilage surfaces was involved, ensuring effective biphasic lubrication. Therefore the depletion of GAGs had a smaller effect on the coefficient of friction for the static model. Indentation tests showed that GAG-depleted cartilage samples had a lower elastic modulus and higher permeability than native tissue. These results corroborate the role of GAGs in the compressive and friction properties of articular cartilage and emphasize the need for developing strategies to control GAG loss from diseased articular cartilage tissue.