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151.
152.
DG Ferris 《Canadian Metallurgical Quarterly》1998,57(6):1215-8, 1228
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Myelin-associated inhibitors of neurite growth play an important role in the regenerative failure after injury in the adult mammalian CNS. The application of the mAb IN-1, which efficiently neutralizes the NI-250/35 inhibitory proteins, alone or in combination with neurotrophin-3 (NT-3), has been shown to promote axonal regeneration when applied in acute injury models. To test whether IN-1 application can induce axonal growth also in a chronic injury model, we treated rats with IN-1 and NT-3 starting 2 or 8 weeks after injury. Rats underwent bilateral dorsal hemisection of the spinal cord at the age of 5-6 weeks. Regeneration of corticospinal (CST) fibers into the caudal spinal cord was observed in three of eight of those animals with a 2-week delay between lesion and treatment. CST fibers regenerated for 2-11.4 mm. In the control group sprouting occurred rostral to the lesion but no long-distance regeneration occurred. In animals where treatment started at 8 weeks after injury the longest fibers observed grew up to 2 mm into the caudal spinal cord. The results show that transected corticospinal axons retain the ability to regenerate at least for a few weeks after injury. Functional analysis of these animals showed a slight improvement of functional recovery. 相似文献
157.
DG Nabavi DW Droste G Schulte-Altedorneburg V Kemény M Panzica S Weber EB Ringelstein 《Canadian Metallurgical Quarterly》1998,66(10):466-473
AIM OF THE STUDY: To evaluate the potential and limitations of echocontrast enhancement using Levovist in a non selected consecutive cohort of neurological patients with insufficient native ultrasound investigations. METHODS: In 91 patients an indication for echocontrast application was seen after an insufficient extracranial (n = 17), transtemporal (n = 54), and transforaminal (n = 20) Doppler- und color-coded Duplex sonography. Levovist was injected at a concentration of 400 mg/ml and 200-400 mg/ml for the transcranial and extracranial approach, respectively. The effect of the echocontrast enhancement was assessed semiquantitatively with respect to signal enhancement, imaging quality, and diagnostic confidence. RESULTS: In a total of 83 patients (91%) the signal enhancement led to a moderate to high imaging quality allowing to reach 67 definite neurovascular diagnoses (74%). In subgroup analysis, the amount of sufficiently confident examinations was significantly higher for the transtemporal and transforaminal (both 80%) than for the extracranial approach (47%). The latter was mostly due to artificial signals derived from adjacent neck vessels. CONCLUSION: Levovist constitutes a safe and highly effective diagnostic tool especially for the transtemporal and transforaminal neurosonographical imaging. By means of a differentiated application of echocontrast agents, its cost-effectiveness can be increased and the need for other potential invasive and expansive neuroimaging methods can be further reduced. 相似文献
158.
B Nicol DG Lambert DJ Rowbotham E Okuda-Ashitaka S Ito D Smart AT McKnight 《Canadian Metallurgical Quarterly》1998,356(2-3):R1-R3
We have examined the effects of the recently described heptadecapeptide nocistatin on K+-evoked glutamate release from rat cerebrocortical slices in vitro. In vivo, nocistatin reverses the action of nociceptin. Nocistatin (100 nM, n = 7) did not inhibit K+-evoked glutamate release alone. Nociceptin (100 nM) inhibited glutamate release by 51.7 +/- 8.3% (P < 0.05, n = 6) and this was fully reversed by nocistatin (100 nM). Nocistatin also appears to be an antagonist of nociceptin action in vitro. 相似文献
159.
The flame tubes from the domestic heating devices made of different Ni-based alloys (alloy 601/NiCr23Fe and even alloy 602/NiCr25FeAlY) showed after a short operation time severe attack by metal dusting. This phenomenon occurred only during combustion of the fuel oil with a new chemical composition. The german market offers such fuel oils with a reduced sulphur content due to more severe environment regulations. Sulphur is able to stabilize the cementite suppressing or retarding the appearance of metal dusting. Metallographical studies revealed that under this operation conditions the oxide scale on the Ni-based alloy forms only partially.In order to prevent the appearance of metal dusting, the flame tubes were coated by a high performance layer (MCrAlY-alloy) which shows a good stability under these corrosive conditions. Uncoated and MCrAlY-coated samples made of alloy 601 and alloy 602 were used for metal-dusting experiments. The specimens were tested using a computer-controlled special equipment which was developed and built in order to simulate the parameters in a flame tube of an oil heating device.Metallographical investigations of the tested samples revealed that the oxide scale present on the uncoated based alloy was formed only partially, whereas in the case of the MCrAlY coatings the oxide scale has an uniform distribution and a good stability due to the presence of the β-NiAl phase which is considered the Al-reservoir for its formation. 相似文献
160.
The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel beta-sheet flanked by two alpha-helices on one side and one alpha-helix and one 3(10)-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (approximately 152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA. 相似文献