Relative importance of the lift force on heavy particles due to turbulence driven secondary flows

Saffman lift forces on dense particles due to gradients in both streamwise and cross-stream velocities in a downward, fully developed turbulent square duct flow at Re_τ = 360 are studied using large eddy simulations. Volume fraction of the dispersed phase is low enough (≤ 10^− 5) that the one-way coupling approach is reasonable, i.e., two-way coupling and particle-particle collisions are not considered. Eulerian and Lagrangian approaches are used to treat the continuous and dispersed phases, respectively. Subgrid stresses are modeled with the dynamic subgrid kinetic energy model of Kim and Menon [W.W. Kim and S. Menon. Application of the localized dynamic subgrid-scale model to turbulent wall-bounded flows, AIAA 97-0210, 1997.]. The particle equation of motion includes drag, lift forces due to both the streamwise and cross-stream velocity gradients, gravity, and is integrated using the fourth-order accurate Runge-Kutta scheme. Dependence of particle drag and lift forces on duct cross-sectional location and particle response time is demonstrated using the mean value contours and probability density functions (PDFs) of particle forces. It is shown that the streamwise component of the mean drag force experienced by particles of all response times is a deceleration force, i.e. on average, fluid streamwise velocity lags the particle streamwise velocity. Secondly, the two wall-normal (or lateral) components of the mean drag force are oriented such that the particles experience a net mean force toward the duct corners. PDFs of particle drag force components show that smaller response time particles experience a wider range of drag force about the mean value, as compared to the more inertial particles. Contours of mean wall-normal lift forces due to streamwise velocity gradients show that this force predominantly acts toward the duct walls and that the maximum lift force occurs close to the walls. PDFs of lift force due to streamwise velocity gradients show that the range of fluctuations increases with particle response time, but the dependence on particle response time is weaker compared to drag force. Lift forces due to cross-stream velocity gradients are at least an order of magnitude smaller than lift forces due to streamwise velocity gradients and are found to decrease in their range of fluctuations with particle response time. It is demonstrated that lift forces due to secondary flow velocity gradients are not as important as those due to streamwise velocity gradients in a square duct flow.     

Production of the Carboxylate Reductase from Nocardia otitidiscaviarum in a Soluble,Active Form for in vitro Applications

Accessing aldehydes from carboxylate moieties is often a challenging task. In this regard, carboxylate reductases (CARs) are promising catalysts provided by nature that are able to accomplish this task in just one step, avoiding over-reduction to the alcohol product. However, the heterologous expression of CARs can be quite difficult due to the excessive formation of insoluble protein, thus hindering further characterization and application of the enzyme. Here, the heterologous production of the carboxylate reductase from Nocardia otitidiscaviarum (NoCAR) was optimized by a combination of i) optimized cultivation conditions, ii) post-translational modification with a phosphopantetheinyl transferase and iii) selection of an appropriate expression strain. Especially, the selection of Escherichia coli tuner cells as host had a strong effect on the final 110-fold increase in the specific activity of NoCAR. This highly active NoCAR was used to reduce sodium benzoate to benzaldehyde, and it was successfully assembled with an in vitro regeneration of ATP and NADPH, being capable of reducing about 30 mM sodium benzoate with high selectivity in only 2 h of reaction.     

Combining Electricity and Fuel Supply: Operational Strategies for Biogas Plants

The operational consequences resulting from the combination of a flexibly operated combined heat and power unit (CHP) and an off‐grid biomethane filling station at one biogas plant were investigated. Four different operating scenarios were compared to evaluate the storage capacity needed to balance biogas demand fluctuations and biogas production. The scenario in which fuel production was given priority and electricity was produced within the remaining hours proved to cause the lowest additional costs since it does not require additional installation of biogas storage capacity and causes minor opportunity cost in electricity marketing. Giving fuel demand the priority reduces the necessary biogas storage capacity by 75 % compared to parallel optimization of the electricity and fuel demands.     

A Donor Agency Scramble for South Africa

The international development community invests bulk of its financial aid to South Africa in establishing procedural conditions through which development actions may be realized. Accordingly, ‘democracy assistance’ is designed to target governmental structures and civil society organizations (CSOs). International donor governments and aid agencies also assign an operational role to civil society so that it may assist in the process of change. However, an overt emphasis on the procedural aspects of democracy, governance, and development ignores the context and object of planning. Consequently, development planning in South Africa is perceived in narrow procedural terms alone, and not in terms of more holistic and equitable development responses.     

Technology learning for renewable energy: Implications for South Africa's long-term mitigation scenarios

Technology learning can make a significant difference to renewable energy as a mitigation option in South Africa's electricity sector. This article considers scenarios implemented in a Markal energy model used for mitigation analysis. It outlines the empirical evidence that unit costs of renewable energy technologies decline, considers the theoretical background and how this can be implemented in modeling. Two scenarios are modelled, assuming 27% and 50% of renewable electricity by 2050, respectively. The results show a dramatic shift in the mitigation costs. In the less ambitious scenario, instead of imposing a cost of Rand 52/t CO₂-eq (at 10% discount rate), reduced costs due to technology learning turn renewables into negative cost option. Our results show that technology learning flips the costs, saving R143. At higher penetration rate, the incremental costs added beyond the base case decline from R92 per ton to R3. Including assumptions about technology learning turns renewable from a higher-cost mitigation option to one close to zero. We conclude that a future world in which global investment in renewables drives down unit costs makes it a much more cost-effective and sustainable mitigation option in South Africa.     

Beta-glucuronidase in human mammary carcinomas

The result of prompt squatting is an increase of the venous return and of the arterial resistance. Consequences are increasing of the end-diastolic and systolic chamber pressure, stroke volume, average arterial blood pressure as well as decreasing of the frequency of the pulse controlled by baro-receptors. The regulatory mechanism both change the intensity of heart murmurs of various cardiopathies and of functional heart murmurs in children. Only the systolic murmur of muscular subvalvular aortic stenosis softens. The experiment is most valuable for the recognition of mild insufficiency of semilunar valves.