Localization of a low abundance membrane protein (Bm86) on the gut cells of the cattle tick Boophilus microplus by immunogold labeling

A preembedding immunogold technique was used to locate Bm86, an antigen from the gut digest cells of the cattle tick Boophilus microplus. Gut from partially engorged female ticks was everted to expose the cells, lightly fixed in 4% paraformaldehyde, and then incubated in rabbit antisera against a recombinant form of Bm86. Following incubation in a secondary antibody conjugated to 1-nm colloidal gold, Bm86 antigenic sites were visualized for both light and electron microscopy using silver enhancement. Bm86 was shown to be located predominantly on the microvilli of digest cells. Antiserum against a nonglycosylated Escherichia coli recombinant form of Bm86 was used to avoid cross-reactivity with carbohydrate epitopes of other digest cell proteins.     

Performance of simulcast wireless techniques for personalcommunication systems

Broadband analog transport facilities using fiber or fiber/coax cable can play a significant role in the evolution of the network infrastructure for personal communications services (PCSs). Low-power PCS systems require a dense grid of radio ports to provide connectivity to the telephone network. Analog transport has a number of important advantages over digital transmission facilities, including the flexibility to support a variety of air interface formats, shared infrastructure cost with other services such as video distribution, and centralized call processing allowing the use of low cost and simple radio ports. A simulcast technique can be used in such systems to permit low rates of handoff (no handoff within each simulcast area) and sharing of hardware resources among multiple radio ports. This paper provides a detailed model and a simulation analysis of the cochannel interference and noise performance as well as the resource sharing benefit of a simulcast PCS system. Several potential PCS air interfaces are considered, including time division multiple access (TDMA) and code division multiple access (CDMA) techniques. Our investigation shows that the impact of multiple antenna noise in a simulcast system is offset by the improved signal-to-interference (SIR) ratio brought about by distributed antennas. Even with distributed antennas, multiple antenna noise places a limit on the maximum number of radio ports that can be assigned to each simulcast group. This limit, however, is shown to have little impact on the achievable resource sharing benefit of simulcasting (i.e., grouping beyond this limit has diminishing returns). A saving of 40% to 60%, in terms of the required central hardware resources, is typical for both TDMA and CDMA systems in suburban environments     

The prevalence of oral candidiasis in HIV-infected individuals and dental attenders in Edinburgh

This study prospectively assessed the prevalence of oral candidal carriage and oral candidiasis in known HIV-seropositive individuals (n = 121) and other dental attenders in Edinburgh (n = 614). Candida species were isolated from 57.4% of dental attenders and 93.4% of HIV-seropositive subjects. Clinical evidence of oral candidiasis was observed in 6% and 52% of these groups respectively, erythematous forms of candidiasis being the commonest in both groups.     

Distributed systems and their protocols

Several models have been developed to assist with the design and understanding of distributed systems. The client-server model is used here to develop a set of requirements to be met by protocol sets used within a distributed system. An architecture within which protocols may be implemented to meet the requirements developed is proposed. A number of key requirements are reviewed and areas requiring further development identified.     

Different cardiovascular profiles of three melanocortins in conscious rats; evidence for antagonism between gamma 2-MSH and ACTH-(1-24)

Mutants of Escherichia coli and Saccharomyces cerevisiae that lack O6-alkylguanine-DNA alkyltransferase activities have increased spontaneous mutation rates, indicating the presence of a cellular metabolite that can alkylate DNA. Bacterially catalysed nitrosation has been implicated previously in producing the endogenous alkylating agent(s). Here, nitrosated polyamines and azaserine, a model compound for nitrosated peptides, are shown to be mutagenic to E. coli ada ogt mutants deficient in O6-alkylguanine-DNA alkyltransferase activity. The mutagenicity of azaserine may be explained by its ability to methylate DNA, whereas nitrosated spermidine causes DNA damage that is susceptible to both nucleotide excision repair and O6-alkylguanine-DNA alkyltransferase activity, which indicates the generation of more bulky DNA adducts. Nitrosated peptides and polyamines are therefore potential endogenous mutagens that are harmful particularly in O6-alkylguanine-DNA alkyltransferase deficient cells.