A paradigm of experimentally induced mild hyperthyroidism: effects on nitrogen balance, body composition, and energy expenditure in healthy young men

OBJECTIVE: To conceptualize, with fine needle aspiration cytology (FNAC), the early cellular events occurring in and around fresh autogenous and allogenic bone grafts during the first 40 postimplantation days. STUDY DESIGN: Forty-eight cases of bone grafts were studied by FNAC at serial intervals of 10, 20, 30 and 40 postimplantation days. Twenty patients were recipients of autogenous grafts, 16 received 0.6N HCI partially decalcified allogenic bone implants, and 4 received combined autogenous and allogenic bone grafts (included in the allograft group). There were eight control cases of closed fracture shaft femur, which were managed conservatively. RESULTS: The initial cellular responses in autogenous grafts, allografts and controls appear to be a part of the nonspecific reparative process followed by a more specific phase, with a steady increase in relative lymphocyte count from the 20th day onwards. Osteogenesis, as judged by osteoblasts and osteoclasts, was also comparable. CONCLUSION: Partially decalcified allografts appear to be a good substitute for autogenous bone grafts in clinical practice when adequate autogenous material is not available. FNAC is a good technique for studying bone graft responses without interfering with graft uptake. It is helpful in the early detection of subclinical infection or any other pathology at the graft site.     

Herpes simplex virus seropositivity and reactivation at delivery among pregnant women infected with human immunodeficiency virus-1

OBJECTIVE: Our purpose was to determine whether pregnant women infected with human immunodeficiency virus-1 have an increased risk of herpes simplex virus-2 seropositivity and herpes simplex virus reactivation at delivery. STUDY DESIGN: Sixty women infected with human immunodeficiency virus and 8408 other patients who were delivered at the University of Washington between 1989 and 1995 had herpes simplex virus serologic determinations at delivery. Genital herpes simplex virus cultures were obtained for 48 (80%) of the human immunodeficiency virus-infected women and 5567 (66%) of the controls. Logistic regression was used to adjust for possible confounding factors. RESULTS: Forty-five (75%) of human immunodeficiency virus-infected women and 2709 (32%) controls were seropositive for herpes simplex virus-2 (p < 0.0001). Eight percent of human immunodeficiency virus-infected women and 2% of controls had herpes simplex virus reactivation in labor (p < 0.05). CONCLUSIONS: Infection with herpes simplex virus-2 is common among pregnant women infected with human immunodeficiency virus. Herpes simplex virus reactivation complicates labor in this group more often than in other obstetric patients. The role of herpes simplex virus in perinatal human immunodeficiency virus transmission warrants further study.     

Getting along and getting ahead: Empirical support for a theory of protective and acquisitive self-presentation.

Study 1, with 386 undergraduates, demonstrated that the Concern for Appropriateness Scale and the Revised Self-Monitoring Scale (SMS—R) developed by the 1st 2 authors (see record 1984-27678-001) were orthogonal and that they were predictably related to measures of self-esteem, social anxiety, shyness, and sociability. Study 2, with an additional 249 Ss, showed that Ss high in concern for appropriateness tended to describe their own drug use as due to inducement by others, whereas Ss who scored high on the SMS—R tended to describe their drug use as self-initiated. (29 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Improving mass spectrometric sequencing of arginine-containing peptides by derivatization with acetylacetone

Modification of arginine residues in bradykinin, [1-5]-bradykinin, splenopentin and two synthetic pentapeptides with acetylacetone (pentane-2,4-dione) significantly increases the relative abundance of sequence-specific fragment ions produced by matrix-assisted laser desorption/ionization (MALDI). The fragmentation efficiency as measured by post-source decay in a reflectron time-of-flight mass spectrometer increases by a factor of 2-3.5. Peptide bonds adjacent to modified residues are more susceptible to cleavage than in the non-derivatized peptide ions. The increased lability of these bonds gives rise to more complete sequence information. In addition, the relative abundances of sequence-specific fragment ions are enhanced. This strategy makes it possible to obtain valuable structural information from arginine-containing peptides that otherwise do not fragment well.     

Negative affectivity in children: A multitrait-multimethod investigation.

The present study investigated the use of the broad-band construct, negative affectivity, with children's self-report measures of depression, anxiety, and anger. A multitrait-multimethod analysis was conducted to intercorrelate scores from self-report measures with scale and subscale scores of the Child Behavior Checklist–Teacher Report Form ({cbcl-trf}). The resulting matrix revealed significant correlations among the self-report measures of anxiety and depression and revealed that these measures correlated significantly with the Internalizing scale scores of the {cbcl-trf}. However, correlations between the individual self-report measures and their corresponding {cbcl-trf} scale scores were not significant. Regression analyses indicated that both depression and anxiety self-report measures were predictors of the {cbcl-trf} Internalizing scale, but no self-report measures were predictive of the {cbcl-trf} Externalizing scale. Findings suggest that the child self-report measures of anxiety and depression used in this study can best be conceptualized as measures of a broad-band construct rather than of the narrow-band dimensions of anxiety and depression. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Progression of interproximal caries in the primary dentition

In the patients with invasion to the aortic window, we performed operation via median sternotomy combined with anteroaxillar thoracotomy. In such patients with T4 invasion, conventional pneumonectomy could not be performed because of the extensive invasion near the main pulmonary artery trunk. In these patients in this study, complete resection of the involved pulmonary artery could be performed using a vascular clamp without CP bypass. Operative technique was as follows: first, the pericardium was opened and taping of the aorta was applied. When the uninvolved part of the intrapericardial pulmonary artery was long enough to cut, we could use a stapling device, but the stapling device could not be used in many cases because the length of the uninvolved segment was too short to cut the left pulmonary artery. In order to carry out complete resection, it was necessary to clamp the central part of the main pulmonary artery diagonally from the left lower side to the right upper side. The pulmonary arterial stump was closed with continuous 4-0 monofilament mattress and over and over suture. We recommend an aggressive surgical approach for the tumor with invasion to the aortic window, because the prognosis is dismal in nonresected locally advanced lung cancer.     

A parallel finite-element tearing and interconnecting algorithm forsolution of the vector wave equation with PML absorbing medium

A domain decomposition method based on the finite-element tearing and interconnecting (FETI) algorithm is presented for the solution of the large sparse matrices associated with the finite-element method (FEM) solution of the vector wave equation. The FETI algorithm is based on the method of Lagrange multipliers and leads to a reduced-order system, which is solved using the biconjugate gradient method (BiCGM). It is shown that this method is highly scalable and is more efficient on parallel platforms when solving large matrices than traditional iterative methods such as a preconditioned conjugate gradient algorithm. This is especially true when a perfectly matched layer (PML) absorbing medium is used to terminate the problem domain     

Drying method and origin of standard affect condensed tannin (CT) concentrations in perennial herbaceous legumes using simplified butanol‐HCl CT analysis

BACKGROUND: Several factors affect condensed tannin (CT) levels in plants and accuracy of the butanol‐HCl assay for total CT. Six native, perennial, herbaceous legumes from Texas were harvested at three stages of growth over a growing season; young vegetation, initial flowering, and late season. The samples were subjected to oven‐drying and freeze‐drying and analyzed for extractable (ECT), protein‐bound (PBCT), and fiber‐bound (FBCT) CT using a butanol‐HCl procedure, comparing several types of purified CT as standards. RESULTS: There was a wide range of CT concentration in the legumes, but little change in ECT and total (TCT) tannin concentrations over the growing season. Oven‐drying decreased (P < 0.05) ECT and increased (P < 0.05) PBCT and FBCT concentrations compared with freeze‐drying, but both methods ranked the forages similarly throughout the growing season relative to TCT. Quebracho tannin as a standard increased CT values for the forages compared with purified legume CT standards, but all standards ranked the forages similarly relative to tannin concentration. Modifications to the procedure increased speed and ease of using the CT analysis method. CONCLUSION: Quantitative CT values have limited value except to allow a relative ranking of forages. Selection of appropriate standards is important in providing realistic CT values. Improvements in the total (extractable + bound) CT analysis method will allow wider use of this procedure. Although ECT and TCT values changed little in native legumes over a growing season, biological activity and potential nutritional/medicinal benefits of CT in these plants needs further evaluation. Copyright © 2008 Society of Chemical Industry