Crystallization and preliminary X-ray diffraction studies of the soluble 14 kDa beta-galactoside-binding lectin from bovine heart

The soluble 14 kDa beta-galactoside-binding lectin from bovine heart, a member of the S-type lectin family, has been crystallized in a form suitable for X-ray diffraction analysis. The crystals, in the absence of a saccharide ligand, diffract beyond 2.5 A resolution. They are obtained from polyethylene glycol 6000 at pH 6.0. Crystals grow as monoclinic plates, space group P2(1), with cell dimensions: a = 35.47 A, b = 64.33 A, c = 58.78 A and beta = 91.7 degrees. The asymmetric unit contains two molecules related by a 2-fold non-crystallographic axis. Two lectin monomers in the asymmetric unit give a Vm of 2.4 A3/Da, i.e. a solvent content of approximately 50%. The complex of lectin with the saccharide ligand, N-acetyllactosamine, crystallizes in the space group P2(1)2(1)2 with cell dimensions: a = 63.55 A, b = 82.13 A and c = 62.39 A. Crystals of this complex diffract beyond 2.0 A resolution. Two complexes in the asymmetric unit lead to a Vm value of 2.8 A3/Da (57% solvent).     

Peer evaluation. A visual picture

To meet the criteria of role accountability, nursing competence and ongoing staff development, an improved peer-evaluation system was designed to continuously monitor these new roles. This peer-evaluation process converts evaluation input into data, giving staff a visual picture of how their performance compares within their peer group. This peer-evaluation process was designed as a tool to assist in staff growth and development, not as a punitive system.     

Gel permeation chromatography: Automatic data acquisition and reduction in real-time with a process computer

A real-time computer system is described which minimizes the total analysis time in gel permeation chromatography by automatically acquiring the data, performing the necessary calculations and displaying the molecular weight distribution and molecular weight averages as soon as the polymer peak has eluted. The calculation method described here is valid for linear homopolymers only, and does not include corrections for instrumental peak broadening. The experimental results show good reproducibility and precision. On-line changes of the constants and other parameters may be implemented by means of an auxiliary program which runs in parallel with the data analysis program. A system such as that described here is suitable for utilization as a measurement technique in process control applications. The programs are written in CORAL 66 for a Ferranti Argus 700 E computer.     

The contact of elastic regular wavy surfaces

A complete solution in closed form to the elastic contact of a one-dimensional sinusoidal surface with a flat surface was presented by Westergaard in 1939. This paper is concerned with the elastic contact of a two-dimensional sinusoidal surface with a flat. In this case the stress distribution within the elastic solids is three-dimensional. As the load is increased the contact areas change in shape from being circular to square and finally leave a circular region of no contact when the waves are almost squashed flat. The problem is solved in general using a numerical method due to Kalker, but asymptotic solutions in closed form have been found for light loads and also for heavy loads at which contact is almost complete. The variation of the mean separation with load, which determines the volume of the space trapped between the two surfaces, is also found.     

Admiral John A. B. Dahlgren (1809–1870): His life, times and technical work in U.S. naval ordnance

John Dahlgren served in the U.S. Navy for nearly 35 years, sometimes in a sea-keeping capacity but mostly developing large naval artillery. To-day, his name, when recognised by students of naval ordnance, is associated with teh Dahlgren or ‘soda bottle shaped’ gun of 9 to 11 inch calibre. He designed, developed and lived to see his guns installed aboard warships and used to considerable effect, especially in the American Civil War. However, a nearly indistinguishable external design of gun was developed for the Army by T. J. Rodman, an officer now even less known than Dahlgren and whose researches and contributions are virtually forgotten. This paper and that in Ref. [1] attempt to appreciate (along with other technical questions of the period) the different ways pursued by these men towards the final development of the ‘soda bottle shaped’ gun, though it is a matter still not entirely understood.

Both names are totally omitted from several ‘classic’ books on artillery and this is a grave misfortune. Besides trying to throw factual light on the Dahlgren-Rodman controversy and the priority issue (though not one pursued with acrimony by the men themselves at the time), the paper describes Admiral Dahlgren's general career, the books he wrote, the technical problems he attacked and the problematic ordnance discussions of his day, to which he gave his life.

The work of Dahlgren and Rodman, in particular, saw the elevation of American gun design from the level of mere copying (of early Columbiads in the first decades of the century) to that which compelled attention from, and study by, the super-powers of the day, Great Britain, France and Russia.     

Current and future water issues in the Oldman River Basin of Alberta, Canada.

Long-term trends in alpine and prairie snow pack accumulation and melt are affecting streamflow within the Oldman River Basin in southern Alberta, Canada. Unchecked rural and urban development also has contributed to changes in water quality, including enhanced microbial populations and increased waterborne pathogen occurrence. In this study we look at changing environment within the Oldman River Basin and its impact on water quality and quantity. The cumulative effects include a decline in net water supplies, and declining quality resulting in increased risk of disease. Our data indicates that decreases in the rate of flow of water can result in sedimentation of bacterial contaminants within the water column. Water for ecosystems, urban consumption, recreation and distribution through irrigation is often drawn from waterholding facilities such as dams and weirs, and concern must be expressed over the potential for contaminate build-up and disproportionate potential of these structures to pose a risk to human and animal health. With disruption of natural flow rates for water resulting from environmental change such as global warming and/or human intervention, increased attention needs to be paid to use of best management practices to protect source water supplies.