全文获取类型
收费全文 | 4497篇 |
免费 | 34篇 |
专业分类
电工技术 | 12篇 |
综合类 | 1篇 |
化学工业 | 207篇 |
金属工艺 | 17篇 |
机械仪表 | 16篇 |
建筑科学 | 47篇 |
矿业工程 | 1篇 |
能源动力 | 14篇 |
轻工业 | 103篇 |
水利工程 | 9篇 |
石油天然气 | 5篇 |
无线电 | 135篇 |
一般工业技术 | 223篇 |
冶金工业 | 3622篇 |
原子能技术 | 18篇 |
自动化技术 | 101篇 |
出版年
2021年 | 12篇 |
2020年 | 9篇 |
2019年 | 8篇 |
2018年 | 10篇 |
2017年 | 21篇 |
2016年 | 16篇 |
2015年 | 13篇 |
2014年 | 29篇 |
2013年 | 49篇 |
2012年 | 34篇 |
2011年 | 40篇 |
2010年 | 28篇 |
2009年 | 33篇 |
2008年 | 34篇 |
2007年 | 44篇 |
2006年 | 24篇 |
2005年 | 36篇 |
2004年 | 31篇 |
2003年 | 31篇 |
2002年 | 34篇 |
2001年 | 32篇 |
2000年 | 25篇 |
1999年 | 139篇 |
1998年 | 1081篇 |
1997年 | 663篇 |
1996年 | 414篇 |
1995年 | 236篇 |
1994年 | 194篇 |
1993年 | 216篇 |
1992年 | 39篇 |
1991年 | 59篇 |
1990年 | 59篇 |
1989年 | 67篇 |
1988年 | 75篇 |
1987年 | 62篇 |
1986年 | 44篇 |
1985年 | 38篇 |
1984年 | 15篇 |
1983年 | 22篇 |
1982年 | 31篇 |
1981年 | 24篇 |
1980年 | 49篇 |
1979年 | 16篇 |
1978年 | 33篇 |
1977年 | 83篇 |
1976年 | 195篇 |
1974年 | 6篇 |
1973年 | 8篇 |
1972年 | 10篇 |
1968年 | 7篇 |
排序方式: 共有4531条查询结果,搜索用时 15 毫秒
11.
Elements of the lactose operon were used to study parameters affecting gene expression in cultured cells and transgenic animals. A Lac repressor protein containing a nuclear transport signal was shown to inhibit expression of a reporter gene by interacting with lac operator sequences. In cultured cells, operator sequence, operator placement and induction parameters were all shown to be important for obtaining tight repression of a reporter gene followed by high level expression upon induction. Induction levels were also dependent on the reporter gene, with the luciferase gene yielding higher induction levels than the chloramphenicol acetyltransferase gene. In transgenic animals, the lacI mRNA was not detected in the C57BL/6 mouse strain until the animal was exposed to a demethylating agent. After 5-azacytidine treatment, expression of lacI mRNA was detected in the brain, heart, kidney, lung and ovary. In the FVB transgenic mouse strain, expression of lacI mRNA was detected without 5-azacytidine treatment in the kidney, liver, lung, and testes. Preliminary experiments with double transgenic animals containing both lacI and operator/luciferase transgenes showed a decrease in luciferase expression compared to the luciferase-only animals in both tissue extracts and transgenic fetal primary cultures, although IPTG induction was not achieved in these animals or primary cultures. The applicability and challenges of the system for regulation of gene expression are discussed. 相似文献
12.
A Gopal G Li Mandri DL King C Marboe S Homma 《Canadian Metallurgical Quarterly》1994,105(6):1885-1887
Cardiac papillary fibroelastomas are unusual, frond-like growths typically found on cardiac valves, diagnosed incidentally on autopsy or cardiac surgery, but rarely during life. We report a rare case of an aortic valve papillary fibroelastoma detected by transthoracic echocardiography and confirmed by histologic study. 相似文献
13.
M Synnott DL Morse H Maguire F Majid M Plummer M Leicester EJ Threlfall J Cowden 《Canadian Metallurgical Quarterly》1993,111(3):473-481
During October 1992 an increase in the number of isolates of Salmonella mikawasima, a rare serotype, was noted including a cluster of nine cases in the South West Thames region. A case control study was conducted and univariate analysis showed a statistical association between illness and eating at take-away A for cases compared with household controls (P = 0.003) and with neighbourhood controls (P = 0.0245). Cases were also more likely to have eaten kebabs than were controls or average take-away A customers, implicating doner kebabs as the most likely vehicle of infection. Plasmid profile analysis of the nine cases' isolates showed them to be indistinguishable and to be characterized by a single plasmid of approximately 60 MDa. The original source of the Salmonella mikawasima contamination was not determined, but food preparation practices for kebabs at take-away A were insufficient to protect against illness if contaminated. This outbreak was only recognized because of the unusual serotype, but could be an indication of a more widespread problem with doner kebabs. 相似文献
14.
The evaluation of microcomputer programs: an area of debate 总被引:1,自引:0,他引:1
V. M. Johnston 《Journal of Computer Assisted Learning》1987,3(1):40-50
15.
Letters to the editor 总被引:1,自引:0,他引:1
16.
[Correction Notice: An erratum for this article was reported in Vol 31(2) of Canadian Psychology Psychologie Canadienne (see record 2007-08913-001). In the October 1989 issue (Vol. 30, No. 4, p. 697), Arnold Rincover's affiliation with the Ontario Institute for Studies in Education was incorrectly given as Associate Professor. He has been an Extramural Instructor at O.I.S.E.] Reviews the book, The parent-child connection by Arnold Rincover (1988). The parent-child connection is a well written book that offers valuable advice to help parents of young children evaluate and understand their children's behaviour. It also offers useful suggestions on managing child behaviour, although these parenting tips are most likely to be useful to those parents who least need them (i.e., those with numerous personal and social resources, whose children are presenting only minor behavioural difficulties). The two general themes of the book, child behaviour as communication and developmental norms as guidelines for deciding if behaviour is problematic, are well-suited to the purposes of a parent reference book. They offer an appropriate framework for discussing specific child behaviours and helping parents to determine if these behaviours are problematic in their children. This book is a welcome addition to the list of available parenting books, and may prove particularly useful for younger parents in need of accurate and understandable information about normal child development and behaviour. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
17.
DL Ellies DW Stock G Hatch G Giroux KM Weiss M Ekker 《Canadian Metallurgical Quarterly》1997,45(3):580-590
To understand the relationship between the expression and the genomic organization of the zebrafish dlx genes, we have determined the genomic structure of the dlx2 and dlx4 loci. This led to the identification of the zebrafish dlx1 and dlx6 genes, which are closely linked to dlx2 and dlx4, respectively. Therefore, the inverted convergent configuration of Dlx genes is conserved among vertebrates. Analysis of the expression patterns of dlx1 and dlx6 showed striking similarities to those of dlx2 and dlx4, respectively, the genes to which they are linked. Furthermore, the expression patterns of dlx3 and dlx7, which likely constitute a third pair of convergently transcribed genes, are indistinguishable. Thus, the overlapping expression patterns of linked Dlx genes during embryonic development suggest that they share cis-acting sequences that control their spatiotemporal expression. The evolutionary conservation of the genomic organization and combinatorial expression of Dlx genes in distantly related vertebrates suggest tight control mechanisms that are essential for their function during development. 相似文献
18.
V Kumar PM Carabateas JA Dority WG Earley JP Mallamo C Subramanyam LD Aimone B Ault DL DeHaven Hudkins MS Miller 《Canadian Metallurgical Quarterly》1995,38(10):1826-1830
Replacement of the pyridinium ring of 6,11-ethanobenzo[b]quinolizinium cations with thiazolium (4a and 4b) and N-methylimidazolium (4c and 4d) resulted in equipotent compounds in the [3H]TCP binding assay. The corresponding N-methyl-1,2,4-triazolium analogs were less potent in this assay. The thiazolium derivative 4b, with a Ki = 2.9 nM, is being evaluated as a possible neuroprotective N-methyl-D-aspartic acid (NMDA) antagonist. 相似文献
19.
Cationic liposomes bound to plasmid DNA are currently used for in vitro and in vivo gene therapy applications, but such complexes readily form large, heterogeneous aggregates that are not appropriate for pharmaceutical development. More importantly, size heterogeneity makes studies focused on optimizing gene transfer to cells difficult to conduct or understand. For this reason we have evaluated the effect of microprobe sonication on these complexes in an effort to achieve process-controlled size homogeneity. Complexes were prepared using a 7.2 kb reporter plasmid and the following liposomal lipid combinations: DDAB/DOPE (50:50 mol %), DDAB/DOPE/PEG-PE (50:45:5 mol %), DDAB/EPC (50:50 mol %), DDAB/EPC/PEG-PE (50:45:5, 50:40:10, 50:35:15 mol %), DODAC/DOPE (50:50 mol %), and DODAC/EPC (50:50 mol %) (DDAB, dimethyldioctadecylammonium bromide; DOPE, dioleoylphosphatidylethanolamine; PEG-PE, monomethoxypolyethylene glycol2000 succinate- distearoylphosphatidylethanolamine; EPC, egg phosphatidylcholine; DODAC, dioleoyldimethylammonium chloride). The influence of complex composition and lipid:DNA ratio was evaluated. Particle size was determined before and after complexation and again after sonication using the quasi-elastic light scattering technique. DNA integrity was assessed via agarose gel electrophoresis. Finally, gene transfection was evaluated using CHO cells that were transfected in vitro with sonicated and unsonicated complexes. It is established in this study that size reduction can occur, but this is dependent on cationic and neutral lipid composition and, in some cases, lipid:DNA ratio. Surprisingly, the process of sonication leaves a significant percentage of the plasmid DNA intact and capable of in vitro transfection. This study shows that plasmid DNA can be protected from damage due to sonication by liposome complex formation. This may indicate that more common pharmaceutical methods for size reduction which subject particles to mechanical stress may be applicable in preparation of liposome/DNA formulations for in vivo application. 相似文献
20.