Fatal meconium aspiration in spite of appropriate perinatal airway management: pulmonary and placental evidence of prenatal disease

The cytoskeletal components of hamster oocytes, zygotes, and spontaneously activated parthogenotes were examined after immunocytochemical labeling. Microtubules were found only in the anastral, tangentially arranged second meiotic spindle of unfertilized oocytes. Taxol treatment of unfertilized oocytes greatly augmented astral microtubules in both the metaphase II spindle and the cortex. Disruption of the meiotic spindle microtubules with nocodazole resulted in cortical chromosomal scattering. During hamster sperm incorporation and pronuclear formation, no sperm aster was detected in association with the male DNA. Instead, a large overlapping array of microtubules assembled in the cortex. By mitosis, this interphase array disassembled and an anastral metaphase spindle formed. Microtubule and chromatin configurations were also imaged in hamster oocytes injected with human sperm. Astral microtubules were absent from the sperm centrosome. The implications of these results are discussed in relation to the hamster oocyte penetration assay, a test commonly used by in vitro fertilization clinics to demonstrate the fertilizing ability of human sperm. We conclude that since hamsters and humans follow different methods of centrosome inheritance, maternal and paternal, respectively, the hamster may be an inappropriate model for exploring microtubule and centrosomal defects in humans or for assaying postinsemination forms of human male fertility defects.     

Development of a thermal hydraulic analysis code for gas-cooled reactors with annular fuels

Gas-cooled reactors have been highlighted as a promising option for next generation reactor technology. A thermal hydraulic analysis code for gas-cooled reactors has been developed with a heat transfer model of a block element, which is solved implicitly with the helium energy equation. Validation was carried out through comparison with both experimental and analytical results. A computation module for annular fuel rods has been coupled to the code for comparative analyses of an annular fuel-based block element. At normal operation, the annular fuel shows 80 °C lower peak temperature than the solid fuel for the same power in Japan's high temperature engineering test reactor (HTTR), even though the pressure drop is higher in the annular fuel.     

Decision-directed maximum-likelihood estimation of OFDM framesynchronisation offset

The authors propose a method to estimate the synchronisation offset for orthogonal frequency division multiplexing (OFDM) frame alignment without resort to pilot tones. A decision-directed maximum-likelihood estimation of frame synchronisation offset is derived, and the performance of the proposed scheme is confirmed by computer simulation for QAM systems     

Mutations can cause large changes in the conformation of a denatured protein

Deletion of 13 amino acids from the carboxyl terminus of staphylococcal nuclease (WTSNase delta) results in a denatured, partially unfolded molecule that lacks significant persistent secondary structure but is relatively compact and monomeric under physiological conditions [Shortle & Meeker (1989) Biochemistry 28, 936-944; Flanagan et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 748-752]. Because of these and other properties of the SNase delta polypeptide, it is a useful model system for investigating the conformation of the denatured state of a protein without using extreme temperature or solvent conditions. Moreover, since the modification is a carboxyl-terminal deletion, SNase delta may also resemble a transient state of the polypeptide chain as it emerges from a ribosome prior to its folding. In the present study, we have examined the sizes and conformations of mutated forms of SNase delta, using small-angle X-ray scattering and circular dichroism spectroscopy. Seven mutated forms were studied: four with single substitutions, two with double substitutions, and one triple substitution. When present in the full-length SNase, each of these mutated forms exhibited unusual behavior upon solvent or thermal denaturation. In the case of the truncated form (SNase delta), the small-angle scattering curves of the mutated forms fall into two classes: one resembling the scattering curve of compact native nuclease and the other having features consistent with those expected for an expanded coil-like polymer. In contrast, the scattering curve of WT SNase delta exhibits features intermediate between those observed for globular proteins and random polymers. The amino acid substitutions that gave rise to compact, native-like versions of SNase delta were all of the m--type (m-substitutions are predicted to decrease the size of the denatured state). Those which gave rise to versions of SNase delta that were more extended and coil-like than WT SNase delta were of the m+ type (m+ substitutions are predicted to increase the size of the denatured state). Estimates of the residual secondary structure present in WT SNase delta, as well as both the m+ and m-substituted versions of SNase delta, as determined by CD, suggest that the formation of secondary structure and compaction of the polypeptide chain occur concurrently. Our results show that single amino acid substitutions can radically alter the conformational distribution of a partially condensed polypeptide chain.(ABSTRACT TRUNCATED AT 400 WORDS)     

Classification of pixels in a noisy grayscale image of polar ice

Often, in synthetic aperture radar (SAR) images of polar ice, one encounters shadow-like features across the images. Such features make it difficult to classify pixels into ice and water. Accordingly, it becomes a challenge to determine the true size and boundaries of ice floes in an SAR image of polar ice. We develop a simple statistical procedure which classifies pixels of an image by eliminating the effects of shadow-like features. Methodology developed in this paper is illustrated using some noisy SAR images of ice floes in the Arctic sea.     

Physical model of drain conductance, gd, degradation ofNMOSFET's due to interface state generation by hot carrierinjection

Degradation of analog device parameters such as drain conductance, g_d, due to hot carrier injection has been modeled for NMOSFET's. In this modeling, mobility reduction caused by interface state generation by hot carrier injection and the gradual channel approximation were employed. It has been found that g_d degradation can be calculated from linear region transconductance, g_m, degradation which is usually monitored for hot carrier degradation of MOSFET's. The values of g_d degradation calculated from g_m degradation fit well to the measured values of g_d degradation The dependence of the g_d degradation lifetime on L_eff has been also studied, this model also provides an explanation of the dependence on L_eff. The model is then useful for lifetime predictions of analog circuits in which g_d degradation is usually more important than g_m degradation