ORK1, a potassium-selective leak channel with two pore domains cloned from Drosophila melanogaster by expression in Saccharomyces cerevisiae

A K+ channel gene has been cloned from Drosophila melanogaster by complementation in Saccharomyces cerevisiae cells defective for K+ uptake. Naturally expressed in the neuromuscular tissues of adult flies, this gene confers K+ transport capacity on yeast cells when heterologously expressed. In Xenopus laevis oocytes, expression yields an ungated K(+)-selective current whose attributes resemble the leak conductance thought to mediate the resting potential of vertebrate myelinated neurons but whose molecular nature has long remained elusive. The predicted protein has two pore (P) domains and four membrane-spanning helices and is a member of a newly recognized K+ channel family. Expression of the channel in flies and yeast cells makes feasible studies of structure and in vivo function using genetic approaches that are not possible in higher animals.     

Dynamic Maps for Exploring and Browsing Shapes

Large datasets of 3D objects require an intuitive way to browse and quickly explore shapes from the collection. We present a dynamic map of shapes where similar shapes are placed next to each other. Similarity between 3D models exists in a high dimensional space which cannot be accurately expressed in a two dimensional map. We solve this discrepancy by providing a local map with pan capabilities and a user interface that resembles an online experience of navigating through geographical maps. As the user navigates through the map, new shapes appear which correspond to the specific navigation tendencies and interests of the user, while maintaining a continuous browsing experience. In contrast with state of the art methods which typically reduce the search space by selecting constraints or employing relevance feedback, our method enables exploration of large sets without constraining the search space, allowing the user greater creativity and serendipity. A user study evaluation showed a strong preference of users for our method over a standard relevance feedback method.     

One-Up,One-Back ERM in the Food Supply Chain

ABSTRACT

Repeated outbreaks of E. coli and other food poisonings call attention to the need for fast, accurate tracing capabilities to identify sources of contamination and track contaminated foods to their destinations. Concerns about biological agents contaminating food or beverages led to the U.S. Bioterrorism Act of 2002. This Act requires those in the food supply chain to identify the immediate previous source (“one-back”) of all food received and the immediate subsequent recipient (“one-up”) of all food released, but recordkeeping remains seriously inadequate. In this article, we examine the role of radio frequency identification (RFID) in electronic record management (ERM) to improve supply chain operations and responses to public health crises.