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G. Kleer E. Schffer M. Bodmann J. Kraft Y. Qiang H. Haberland 《Materialwissenschaft und Werkstofftechnik》1998,29(9):545-554
Hard and wear resistant coatings for the moulding and embossing of glasses at elevated temperatures Hard and wear resistant coatings of Titanium Aluminium Nitride TiAlN were deposited on various substrates by the application of different reactive deposition processes: RF-magnetron-sputtering, ion beam-sputtering and by the energetic cluster impact (ECI) process. The deposition of the coatings was performed under variation of biasing conditions and of process parameters such as pressures and flow rates of the process gases argon and nitrogen as well as of energies of species hitting the substrate surfaces. The microstructure particularly the growth morphology of several films was investigated by pictures of film cross sections recorded by transmission electron microscopy. Residual intrinsic film stresses were analyzed by measuring deflections of substrates in an interference optical microscope before and after the deposition of the coatings. By heating coated substrates and in-situ observation of deflections at elevated temperatures dependencies of thermally induced stresses on temperatures and variations of intrinsic stresses due to changes within the films could be analyzed and related to microstructure and growth conditions. In the paper specific characteristics of the deposition processes occuring on the scale both of atoms and of clusters which may contain several thousand of atoms are described and related to microstructure, residual stress states and damaging conditions. Different contributions to residual film stresses are analyzed on the base of theoretical considerations taking into account deposition kinetics and thermomecanical properties. The significance of achieved film properties for application, i. e. for the coating of tools for the manufacturing of optical components by moulding and embossing of glasses is discussed. 相似文献
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DP Kiel RH Myers LA Cupples XF Kong XH Zhu J Ordovas EJ Schaefer DT Felson D Rush PW Wilson JA Eisman MF Holick 《Canadian Metallurgical Quarterly》1997,12(7):1049-1057
Previous studies of the vitamin D receptor (VDR) polymorphisms and bone mineral density (BMD) have suggested that there may be differences in calcium absorption among groups of women with different VDR genotypes, and that the association may be stronger in younger women. To investigate the association between the VDR polymorphisms and BMD, this study was undertaken in the Framingham Study Cohort and a group of younger volunteers. Subjects from the Framingham Study (ages 69-90 years) included those who underwent BMD testing and who had genotyping for the VDR alleles (n = 328) using polymerase chain reaction methods and restriction fragment length polymorphisms with BsmI (B absence, b presence of cut site). A group of younger volunteer subjects (ages 18-68) also underwent BMD testing and VDR genotyping (n = 94). In Framingham Cohort subjects with the bb genotype, but not the Bb or BB genotypes, there were significant associations between calcium intake and BMD at five of six skeletal sites, such that BMD was 7-12% higher in those with dietary calcium intakes greater than 800 mg/day compared with those with intakes < 500 mg/day. The data also suggested that BMD was higher in persons with the bb genotype only in the group with calcium intakes above 800 mg/day. No significant differences were found in the Framingham Cohort for age-, sex-, and weight-adjusted BMD at any skeletal site between those with the BB genotype and those with the bb genotype regardless of 25-hydroxyvitamin D levels or country of origin. In the younger volunteers, BMD of the femoral neck was 5.4% higher (p < 0.05) in the bb genotype group compared with the BB group and 11% higher (p < 0.05) in males with the bb genotype compared with the BB group. There were no significant differences at the lumbar spine. In this study, the association between calcium intake and BMD appeared to be dependent upon VDR genotype. The findings of an association between dietary calcium intake and BMD only in the bb genotype group suggests that the VDR genotype may play a role in the absorption of dietary calcium. Studies that do not consider calcium intake may not detect associations between VDR genotype and BMD. In addition, the association between VDR alleles and BMD may become less evident in older subjects. 相似文献
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A Kuniyasu K Itagaki T Shibano M Iino G Kraft A Schwartz H Nakayama 《Canadian Metallurgical Quarterly》1998,273(8):4635-4641
To identify the binding domain of a new Ca2+ antagonist semotiadil on L-type Ca2+ channels from skeletal muscle, photolabeling was carried out by using an azidophenyl derivative of [3H]semotiadil. Photoincorporation was observed in several polypeptides of membrane triad preparations; the only specific photoincorporation was in the alpha1 subunit of the Ca2+ channel. After solubilization and purification, the photolabeled alpha1 subunit was subjected to proteolytic and CNBr cleavage followed by antibody mapping. Specific labeling was associated solely with the region of transmembrane segment S6 in repeat IV. Quantitative immunoprecipitation was found in the tryptic and the Lys-C/Glu-C fragments of 6.6 and 6.1 kDa, respectively. Further CNBr cleavage of the Lys-C digests produced two smaller fragments of 3.4 and 1.8 kDa that were included in the tryptic and Lys-C/Glu-C fragments. The smallest labeled fragments were: Tyr1350-Met1366 and Leu1367-Met1381 containing IVS6, a possible pore-forming region. The data suggest that semotiadil binds to a region that is overlapped with but not identical to those for phenylalkylamines, dihydropyridines and benzothiazepines. The present study also provides evidence that region IV represents an important component of a binding pocket for Ca2+ antagonists. 相似文献
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Starting with total RNA from spermatogenic cells of Mytilus trossulus and using random priming, we have cloned and sequenced the c-DNAs corresponding to two variants of the sperm-specific protein PLII* (phi 2B). DNA sequencing in conjunction with mass spectrometry and protein sequence data have allowed us to establish that of the three sperm-specific proteins present in the sperm of Mytilus (PL-II*(phi 2B), PL-III (phi 1), PL-IV (phi 3)), the first and the last one are the result of post-translational cleavage of a common precursor. This common precursor is a member of the histone H1 family, and it exhibits inter- and intraspecific microheterogeneity. 相似文献