Web‐based testing: A study in insecurity

Much has been written about the distinct ideas of online testing, in which students take tests and receive immediate feedback on their performance; distance learning, in which telecommunication technology extends the traditional classroom beyond the classroom walls; and multimedia, in which text, sound and graphics are integrated within an application. By using the World Wide Web (WWW) as a medium for administering online tests, immediate feedback can be provided, tests can be administered at a distance, and multimedia resources can be conveniently incorporated into questions. This paper describes Eval, an undergraduate research software prototype in which we explore security issues related to using WWW as a testing medium.     

Peak oxygen uptake and maturation in 12-yr olds

OBJECTIVE: The purpose of this study is to report a new sign, "acinar filling," observed on dynamic MR pancreatography after secretin stimulation in patients with suspected early chronic pancreatitis. CONCLUSION: Acinar filling might reflect tissue hypertension or loss of pancreatic parenchyma compliance or both. This finding is probably an insensitive but specific sign of early chronic pancreatitis.     

The activity of topoisomerase I is modulated by large T antigen during unwinding of the SV40 origin

When simian virus 40 (SV40) large T antigen binds to the virus origin of replication, it forms a double hexamer that functions as a helicase to unwind the DNA bidirectionally. We demonstrate in this report that T antigen can unwind and release an origin DNA single strand of less than full length in the presence of purified human topoisomerase I. The sites nicked by topoisomerase I in the strands released by T antigen during DNA unwinding were localized primarily to the "late" side of the origin, and the template for lagging strand synthesis was preferred significantly over the one for leading strand synthesis. Importantly, these sites were, for the most part, different from the sites nicked by topoisomerase I in the absence of T antigen. These data indicate that T antigen activates topoisomerase I nicking at discrete sites and releases these nicked strands during unwinding. We hypothesize that a single molecule of topoisomerase I can form a functional complex with a double hexamer of T antigen to simultaneously relax and unwind double-stranded origin-containing DNA.     

Sequence-specific DNA binding by EcoKI, a type IA DNA restriction enzyme

The type I DNA restriction and modification enzymes of prokaryotes are multimeric enzymes that cleave unmethylated, foreign DNA in a complex process involving recognition of the methylation status of a DNA target sequence, extensive translocation of DNA in both directions towards the enzyme bound at the target sequence, ATP hydrolysis, which is believed to drive the translocation possibly via a helicase mechanism, and eventual endonucleolytic cleavage of the DNA. We have examined the DNA binding affinity and exonuclease III footprint of the EcoKI type IA restriction enzyme on oligonucleotide duplexes that either contain or lack the target sequence. The influence of the cofactors, S-adenosyl methionine and ATP, on binding to DNA of different methylation states has been assessed. EcoKI in the absence of ATP, with or without S-adenosyl methionine, binds tightly even to DNA lacking the target site and the exonuclease footprint is large, approximately 45 base-pairs. The protection is weaker on DNA lacking the target site. Partially assembled EcoKI lacking one or both of the subunits essential for DNA cleavage, is unable to bind tightly to DNA lacking the target site but can bind tightly to the recognition site. The addition of ATP to EcoKI, in the presence of AdoMet, allows tight binding only to the target site and the footprint shrinks to 30 base-pairs, almost identical to that of the modification enzyme which makes up the core of EcoKI. The same effect occurs when S-adenosyl homocysteine or sinefungin are substituted for S-adenosyl methionine, and ADP or ATPgammaS are substituted for ATP. It is proposed that the DNA binding surface of EcoKI comprises three regions: a "core" region which recognises the target sequence and which is present on the modification enzyme, and a region on each DNA cleavage subunit. The cleavage subunits make tight contacts to any DNA molecule in the absence of cofactors, but this contact is weakened in the presence of cofactors to allow the protein conformational changes required for DNA translocation when a target site is recognised by the core modification enzyme. This weakening of the interaction between the DNA cleavage subunits and the DNA could allow more access of exonuclease III to the DNA and account for the shorter footprint.     

Agile Methodologies: Organizational Adoption Motives,Tailoring, and Performance

Today, organizations tailor the practices from several agile methodologies to serve their particular environment. But are there situations that drive how an organization should tailor methodologies in a particular manner? This article places 12 commonly used agile development practices into a typology based upon whether they are primarily project management focused or software development approach focused and examines how organizations’ motivations for adopting agile impact the practices they adopt. Finally, it explores how a fit between an organization’s motives for agile method adoption and the tailored agile practices it adopts may lead (or not lead) to differences in project performance.     

Relationship between SST gradients and upwelling off Peru and Chile: model/satellite data analysis

The upwelling system off Peru/Chile is characterized by significant mesoscale to submesoscale surface variability that results from the instability of the coastal currents (due to the strong vertical and horizontal shears) and to the marked density cross-shore gradients (associated with the mean upwelling). Here we investigate to what extent upwelling intensity can be inferred from sea surface temperature (SST) derived from remote sensing. As a first step in validation, a comparison between SST observations is performed, which indicates that the 1 km gridded multi-scale ultra-high-resolution (MUR) SST data set is defining a zone of maximum SST gradients closer to shore than the low-resolution National Centers for Environmental Information 0.25° resolution data set. Two model versions, at nominal resolutions of 2 km and 4 km, of the Massachusetts Institute of Technology general circulation model are analysed. A high-resolution version at 2 km is examined for the period 13 September 2011–23 January 2013, while a 4 km version is examined for 6 March 2011–22 April 2013. MUR shows maxima SST gradients in the range of 0.03 ± 0.02 K km^?1 while the model showed higher gradients around 0.05 ± 0.02 K km^?1. Based on coherence spectra, the relationship between upwelling rate (as inferred from the vertical velocity) and SST gradient is documented in the model from intraseasonal to annual timescales. It suggests that changes in SST gradient magnitudes are related to changes in the intensity of coastal upwelling off Peru and Chile. Such a relationship between SST gradients and vertical velocity would allow for the use of satellite-derived SSTs to monitor the intensity of coastal upwelling from the intraseasonal to annual timescales.     

Water‐Quality Assessment of the Lower Grand River Basin,Missouri and Iowa,USA, in Support of Integrated Conservation Practices

The effectiveness of agricultural conservation programmes to adequately reduce nutrient exports to receiving streams and to help limit downstream hypoxia issues remains a concern. Quantifying programme success can be difficult given that short‐term basin changes may be masked by long‐term water‐quality shifts. We evaluated nutrient export at stream sites in the 44 months that followed a period of increased, integrated conservation implementation within the Lower Grand River Basin. These short‐term responses were then compared with export that occurred in the main stem and adjacent rivers in northern Missouri over a 22‐year period to better contextualize any recent changes. Results indicate that short‐term (October 2010 through May 2014) total nitrogen (TN) concentrations in the Grand River were 20% less than the long‐term average, and total phosphorus (TP) concentrations were 23% less. Nutrient reductions in the short term were primarily the result of the less‐than‐average precipitation and, consequently, streamflow that was 36% below normal. Therefore, nutrient concentrations measured in tributary streams were likely less than normal during the implementation period. Northern Missouri streamflow‐normalized TN concentrations remained relatively flat or declined over the period 1991 through 2013 likely because available sources of nitrogen, determined as the sum of commercial fertilizers, available animal manures and atmospheric inputs, were typically less than crop requirement for much of that time frame. Conversely, flow‐normalized stream TP concentrations increased over the past 22 years in northern Missouri streams, likely in response to many years of phosphorus inputs in excess of crop requirements. Stream nutrient changes were most pronounced during periods that coincided with the major tillage, planting and growth phases of row crops and increased streamflow. Nutrient reduction strategies targeted at the period February through June would likely have the greatest impact on reducing nutrient export from the basin. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.     

Alkali‐Metal‐Intercalated Percolation Network Regulates Self‐Assembled Electronic Aromatic Molecules

In the continuously growing field of correlated electronic molecular crystals, there is significant interest in addressing alkali‐metal‐intercalated aromatic hydrocarbons, in which the possibility of high‐temperature superconductivity emerges. However, searching for superconducting aromatic molecular crystals remains elusive due to their small shielding fraction volume. To exploit this potential, a design principle for percolation networks of technologically important film geometry is indispensable. Here the effect of potassium‐intercalation is shown on the percolation network in self‐assembled aromatic molecular crystals. It is demonstrated that one‐dimensional (1D) dipole pairs, induced by dipole interaction, regulate the conductivity, as well as the electronic and optical transitions, in alkali‐metal‐intercalated molecular electronic crystals. A solid‐solution growth methodology of aromatic molecular films with a broad range of stability is developed to uncover electronic and optical transitions of technological importance. The light‐induced electron interactions enhance the charge‐carrier itinerancy, leading to a switchable metal‐to‐insulator transition. This discovery opens a route for the development of aromatic molecular electronic solids and long‐term modulation of electronic efficacy in nanotechnologically important thin films.     

Automatic decomposition and efficient semi-structured meshing of complex solids

In this paper, a novel approach to automatically sub-divide a complex geometry and apply an efficient mesh is presented. Following the identification and removal of thin-sheet regions from an arbitrary solid using the thick/thin decomposition approach developed by Robinson et al. [1], the technique here employs shape metrics generated using local sizing measures to identify long-slender regions within the thick body. A series of algorithms automatically partition the thick region into a non-manifold assembly of long-slender and complex sub-regions. A structured anisotropic mesh is applied to the thin-sheet and long-slender bodies, and the remaining complex bodies are filled with unstructured isotropic tetrahedra. The resulting semi-structured mesh possesses significantly fewer degrees of freedom than the equivalent unstructured mesh, demonstrating the effectiveness of the approach. The accuracy of the efficient meshes generated for a complex geometry is verified via a study that compares the results of a modal analysis with the results of an equivalent analysis on a dense tetrahedral mesh.