The testing of bulls for the polledness gene]

Main regularities have been stated concerning the poll gene inheritance by the progeny of breeding bulls mated with cows of various (homozygous and heterozygous) genotypes by the "poll" or "hornedness" genes.     

The role of protein kinase in C ischemic/reperfused preconditioned isolated rat hearts

Protein kinase C (PKC) has been implicated in the preconditioning-induced cardiac protection in ischemic/reperfused myocardium. We studied the effect of PKC inhibition with calphostin C (25, 50, 100, 200, 400, and 800 nM), a potent and specific inhibitor of PKC, in isolated working nonpreconditioned and preconditioned ischemic/reperfused hearts. In the nonpreconditioned groups, all hearts underwent 30 min of normothermic global ischemia followed by 30 min of reperfusion. In the preconditioned groups, hearts were subjected to four cycles of ischemic preconditioning by using 5 min of ischemia followed by 10 min reperfusion, before the induction of 30 min ischemia and reperfusion. At low concentrations of calphostin C (25, 50, and 100 nM), the PKC inhibitor had no effect on the incidence or arrhythmias or postischemic cardiac function in the nonpreconditioned ischemic/reperfused groups. With 200 and 400 nM of calphostin C, a significant increase in postischemic function and a reduction in the incidence of arrhythmias were observed in the nonpreconditioned ischemic/reperfused groups. Increasing the concentration of calphostin C to 800 NM, the recovery of postischemic cardiac function was similar to that of the drug-free control group. In preconditioned hearts, lower concentrations (< 100 nM) of calphostin C did not change the response of the myocardium to ischemia and reperfusion in comparison to the preconditioned drug-free myocardium. Two hundred and 400 nM of the PKC inhibitor further reduced the incidence of ventricular fibrillation (VF) from the preconditioned drug-free value of 50% to 0 (p < 0.05) and 0 (p < 0.05), respectively, indicating that the combination of the two, preconditioning and calphostin C, affords significant additional protection. Increasing the concentration of calphostin C to 800 nM blocked the cardioprotective effect of preconditioning (100% incidence of VF). The recovery of cardiac function was similarly improved at calphostin C doses of 200 and 400 nM and was reduced at 800 nM (p < 0.05). With 200 and 400 nM of calphostin C, both cytosolic and particulate PKC activity were reduced by approximately 40 and 60%, respectively, in both preconditioned and preconditioned/ischemic/reperfused hearts. The highest concentration of calphostin C (800 nM) resulted in almost a complete inhibition of cytosolic (100%) and particulate (85%) PKC activity correlated with the abolition of preconditioning-induced cardiac protection. In conclusion, calphostin C protects the ischemic myocardium obtained from intact animals, provides significant additional protection to preconditioning at moderate doses, and blocks the protective effect of preconditioning at high concentrations. The dual effects of calphostin C appear to be strictly dose and "enzyme inhibition" related.     

The 3'-terminal sequence of mitochondrial 13S ribosomal RNA

We have examined the 3'-terminal sequence of the "small" structural ribosomal RNA ("13S") of hamster cell mitochondria, using a procedure involving [3H]isoniazide labeling of samples subjected to sequential periodate oxidation and beta-elimination. The terminus was found to be PyUAUUAOH, which is similar, but not identical, to the corresponding terminus of eukaryotic cytoplasmic 18S rRNA.     

Retrodictive quantum optical state engineering

Abstract

Although it has been known for some time that quantum mechanics can be formulated in a way that treats prediction and retrodiction on an equal footing, most attention in engineering quantum states has been devoted to predictive states, that is, states associated with a preparation event. Retrodictive states, which are associated with a measurement event and propagate backwards in time, are also useful, however. In this paper it is shown how any retrodictive state of light that can be written to a good approximation as a finite superposition of photon number states can be generated by an optical multiport device. The composition of the state is adjusted by controlling predictive coherent input states. It is shown how the probability of successful state generation can be optimized by adjusting the multiport device and also a versatile configuration that is useful for generating a range of states is examined.     

Properties of p- and n-Type PbTe Microwires for Thermoelectric Devices

In thermopower measurements, microwires fabricated from as-purchased bulk PbTe exhibits p-type behavior between room temperature and ～600 K. At higher temperatures, it undergoes majority carrier inversion and exhibits n-type behavior. We report on the preparation and properties of potassium oxide and Zn-doped PbTe microwires, which exhibit stable p- and n-type behavior, respectively, between room temperature and 725 K. Thermoelectric figures of merit (ZT) are reported for device components prepared from bundles of such p- and n-type microwires in a glass matrix.     

Modeling the tryptic hydrolysis of pea proteins using an artificial neural network

Experimentally determined values for the degree of hydrolysis (DH) were used with an artificial neural network (ANN) model to predict the tryptic hydrolysis of a commercially available pea protein isolate at temperatures of 40, 45, and 50 °C. Analyses were conducted using the STATISTICA Neural Networks software on a personal computer. Input data were randomized to two sets: learning and testing. Differences between the experimental and calculated DH% were slight and ranged from 0.06% to 0.24%. The performance of the educated ANN was then tested by inputting temperatures ranging from 35 to 50 °C. Very strong correlations were found between calculated DH% values obtained from the ANN and those experimentally determined at all temperatures; the determination coefficients (R²) varied from 0.9958 to 0.9997. The results so obtained will be useful to reduce the time required in the design of enzymatic reactions involving food proteins.     

Multi‐scale Approach to Hydrological Classification Provides Insight to Flow Structure in Altered River System

Rivers are hierarchical systems exhibiting processes and patterns across spatial and temporal scales principally driven by changes in flow. Hydrological indices estimated with mean or median daily flow data (i.e. daily scale) may be insensitive to anthropogenic alteration that imparts sub‐daily variation to flow. Therefore, indices developed at multiple temporal resolutions may provide additional insight into the presence of flow patterns masked by traditional techniques. We characterized the flow regime along the longitudinal gradient of the Platte River, a large Great Plains USA river, using hydrological indices derived with mean daily and sub‐daily flow data and a combination of multivariate statistical techniques. Three unique flow units were evident using daily scale flow data, whereas six unique flow units were evident at the sub‐daily scale. Flow units at both scales were not static, but rather the presence and extent of flow units across the riverscape depended on climate, tributary inflows and human influence. Anthropogenic alteration including hydropeaking was evident at the sub‐daily scale but not at the daily scale. The full complement of flow structure within regulated rivers, therefore, may not be captured using mean or median daily discharge values alone. Inductive river classification studies may benefit from assessing hydrological indices at multiple scales, particularly when investigating river systems with anthropogenic modification such as hydropeaking. Copyright © 2016 John Wiley & Sons, Ltd.     

Bayes' theorem and quantum retrodiction

Abstract

We derive on the basis of Bayes' theorem a simple but general expression for the retrodicted premeasurement state associated with the result of any measurement. The retrodictive density operator is the normalized probability operator measure element associated with the result. We examine applications to quantum optical cryptography and to the optical beam splitter.     

Quantum nature of laser light

All compositions of a mixed-state density operator are equivalent for the prediction of the probabilities of future outcomes of measurements. For retrodiction, however, this is not the case. The retrodictive formalism of quantum mechanics provides a criterion for deciding that some compositions are fictional. Fictional compositions do not contain preparation device operators, that is operators corresponding to states that could have been prepared. We apply this to Mølmer's controversial conjecture that optical coherences in laser light are a fiction and find agreement with his conjecture. We generalize Mølmer's derivation of the interference between two lasers to avoid the use of any fictional states. We also examine another possible method for discriminating between coherent states and photon number states in laser light and find that it does not work, with the equivalence for prediction saved by entanglement.