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131.
Energy requirements of grazing livestock seem to be poorly defined. Requirements generated from measurements in pen-fed animals present both theoretical and practical concerns when they are applied to grazing situations. In mature ruminants a majority of energy used for maintenance seems to be consumed by visceral tissues. Moreover, factors that affect visceral energy use are poorly understood. Energy supplementation to meet requirements and production demands is often practiced during periods of summer dormancy and in winter months. Source of supplemental energy varies widely and includes grains, readily digestible fiber sources, and high-quality forages. Intake and digestibility can be reduced or unaffected by energy supplementation. In some cases, lower levels of energy supplementation have been shown to increase utilization of grazed forage. Reductions in ruminal pH, often cited as the major cause of reduced fiber digestion, may not always explain reductions in intake and digestibility associated with energy supplementation. Livestock production is usually either enhanced or unaffected by energy supplementation. Additional research regarding energy requirements of grazing ruminants, visceral energy use, and mechanisms associated with reductions in intake and digestibility of grazed forage resulting from energy supplementation is warranted. 相似文献
132.
HE Jones CL Eaton D Barrow CM Dutkowski JM Gee K Griffiths 《Canadian Metallurgical Quarterly》1997,30(4):219-231
Murine acute myeloid leukemia is characterized by chromosome 2 aberrations, and genesis of the marker chromosome 2 by radiation is suspected to be an initiating event of radiation leukemogenesis. A detailed analysis of the type and frequency of chromosome 2 aberrations in murine bone marrow cells at an early stage after irradiation is provided here. A total of 40 male C3H/He mice was exposed to 137Cs gamma-ray at a dose of 1, 2 or 3 Gy, and sacrificed 24 hours after irradiation. Metaphase samples prepared from bone marrow cells were Q-banded for karyotyping or painted with DNA probes specific to chromosome 2. In 5 mice analyzed by karyotyping, one mouse showed high frequency of the marker aberrations as well as other chromosome 2 aberrations. Chromosome painting analysis for the rest of the mice also detected 3 animals showing significantly high frequencies of chromosome 2 aberrations. Dose-dependence of the frequencies was observed even among those mice that tended to be sensitive. The results indicated that there was a subgroup of mice carrying hypersensitive chromosome 2. The subgroup could be leukemia-sensitive if radiation-induced chromosome aberrations are responsible for an early change in myeloid leukemogenesis. 相似文献
133.
JA Court S Lloyd M Johnson M Griffiths NJ Birdsall MA Piggott AE Oakley PG Ince EK Perry RH Perry 《Canadian Metallurgical Quarterly》1997,101(1-2):93-105
High-affinity nicotine, alpha-bungarotoxin (alpha BT) and muscarinic receptor binding was measured in the human hippocampal formation in a series of 57 cases aged between 24 weeks gestation and 100 years. Changes in nicotine receptor binding during development and aging were more striking than differences in alpha BT and muscarinic binding. Nicotine binding was higher at the late foetal stage than at any other subsequent time in all areas investigated. In the hippocampus a fall in binding then occurred within the first six months of life, with little or no subsequent fall during aging, whereas in the entorhinal cortex and the presubiculum the major loss of nicotine binding occurred after the fourth decade. alpha BT binding was significantly elevated in the CA 1 region, but in no other region of the hippocampus, in the late foetus, and there was also a fall in alpha BT binding in the entorhinal cortex during aging from the second decade. The modest changes in total muscarinic binding, which appeared to reflect those in M1 and M3 + 4 rather than M2 binding, were a rise in the entorhinal cortex between the foetal stage and childhood and a tendency for receptors to fall with age in the hippocampus and subicular complex. These findings implicate mechanisms controlling the expression of nicotinic receptors to a greater extent than muscarinic receptors in postnatal development and aging in the human hippocampus. 相似文献
134.
Cyclins are essential proteins in cell cycle control, and their deranged expression has been reported to be associated with malignant transformation. Involvement of cyclins in the development of endometrioid carcinomas of the endometrium was studied immunohistochemically using antibodies against both cyclin A and tumor suppressor gene product p53, and their expression was compared with that of Ki-67 antigen. Sixty-two cases of endometrial endometrioid carcinoma and 20 cases of normal endometrium (10 proliferative and 10 secretory phase) were examined. Of the 62 endometrioid carcinomas, atrophic endometrium and hyperplasia were found adjacent to the cancers in 30 and 19 cases respectively. Cyclin A was expressed in < 1% of the glandular cells of normal endometrium in the proliferative phase and in hyperplasia, but was negligible in normal secretory phase and atrophic endometrium. p53 was almost always negative in normal endometrium and hyperplasia. Of the 62 endometrioid carcinomas, 12 tumors (19.4%) overexpressed cyclin A and 21 tumors (33.8%) overexpressed p53 (positive cells > 1%). Cyclin A and p53 were more frequently expressed in poorly differentiated tumors than in well differentiated tumors (cyclin A, p = 0.002; p53, p = 0.016). In addition, cyclin A-positive cells were topographically related to those cells positive for p53 as well as Ki-67. In conclusion, the abnormal expression of cyclin A and p53 is associated with high-grade endometrial endometrioid carcinomas. 相似文献
135.
136.
137.
M W Griffiths 《Journal of the science of food and agriculture》1989,47(2):133-158
Recent outbreaks of listeriosis associated with consumption of milk products contaminated with Listeria monocytogenes have focused attention on the importance of food-borne transmission of this disease. This review outlines current knowledge of the incidence of L monocytogenes in raw milk and its incidence in, and ability to survive, the manufacturing process for other dairy products—notably soft cheeses. Discrepancies in results obtained by researchers concerning growth of the organism at refrigeration temperatures and the bacterium's ability to survive pasteurisation are discussed. 相似文献
138.
Nitric oxide released by macrophages during inflammation reacts with active oxygen to form peroxynitrite. Peroxynitrite nitrates protein and peroxidizes lipids. gamma-Tocopherol traps peroxynitrite and is more effective than alpha-tocopherol in protecting lipids against such peroxidation. 相似文献
139.
OI Potrokhov DV Dolgikh VP Kas'ianovskaia OI Shevchenko 《Canadian Metallurgical Quarterly》1997,(7):14-18
The study covered influence of combined toxic chemicals on fire extinguishers. Acute exposure of respiratory and peripheral nervous systems was demonstrated. Consequences of the exposure were proved to include polyneuropathies, neuroses and other health disorders. 相似文献
140.
A semipermissive growth condition was defined for a Schizosaccharomyces pombe strain carrying a thermosensitive allele of DNA polymerase delta (pol delta ts03). Under this condition, DNA polymerase delta is semidisabled and causes a delay in S-phase progression. Using a genetic strategy, we have isolated a panel of mutants that enter premature mitosis when DNA replication is incomplete but which are not defective for arrest in G2/M following DNA damage. We characterized the aya14 mutant, which enters premature mitosis when S phase is arrested by genetic or chemical means. However, this mutant is sensitive to neither UV nor gamma irradiation. Two genomic clones, rad26+ and cds1+, were found to suppress the hydroxyurea sensitivity of the aya14 mutant. Genetic analysis indicates that aya14 is a novel allele of the cell cycle checkpoint gene rad26+, which we have named rad26.a14. cds1+ is a suppressor which suppresses the S-phase feedback control defect of rad26.a14 when S phase is inhibited by either hydroxyurea or cdc22, but it does not suppress the defect when S phase is arrested by a mutant DNA polymerase. Analyses of rad26.a14 in a variety of cdc mutant backgrounds indicate that strains containing rad26.a14 bypass S-phase arrest but not G1 or late S/G2 arrest. A model of how Rad26 monitors S-phase progression to maintain the dependency of cell cycle events and coordinates with other rad/hus checkpoint gene products in responding to radiation damage is proposed. 相似文献