A PLL clock generator with 5 to 10 MHz of lock range formicroprocessors

A microprocessor clock generator based on an analog phase-locked loop (PLL) is described for deskewing the internal logic control lock to an external system lock. This PLL is fully generated onto a 1.2-million-transistor microprocessor in 0.8-μm CMOS technology without the need for external components. It operates with a lock range from 5 to 110 MHz. The clock skew is less than 0.1 ns, with a peak-to-peak jitter of less than 0.3 ns for a 50-MHz system clock frequency     

Production of the 19-kDa antigen of Mycobacterium tuberculosis in Escherichia coli and its purification

The 19-kDa antigen (19Ag) of Mycobacterium tuberculosis (Mt) is a lipoprotein which is released from the organism during growth. In order to study the possible involvement of this antigen in the host protective response against Mt infection, it would be helpful to obtain high-level production of 19Ag from a recombinant organism. We have found that overexpression of the native 19Ag gene in Escherichia coli or yeast leads to products which are aggregated and insoluble. By site-directed mutagenesis of the 19Ag lipoprotein leader sequence, we have generated a mutant gene which directs the production of 19Ag into the periplasmic space of E. coli, from where it can be easily purified in high yield. 19Ag obtained from this mutant construct lacks the lipid-modified N-terminal Cys residue found in the native 19Ag, and is not glycosylated, but is otherwise indistinguishable from 19Ag isolated from Mt culture supernatant.     

Numerical modeling of near corona wire electrohydrodynamic flow in a wire-plate electrostatic precipitator

Numerical modelling of the flow velocity fields for the near corona wire electrohydrodynamic (EHD) flow was conducted. Solutions of the steady, two-dimensional momentum equations have been computed for a wire-plate type electrostatic precipitator (ESP). The equations were solved in the conservative finite-difference form on a fine uniform rectilinear grid of sufficient resolution to accurately capture the momentum boundary layers. The numerical procedure for the differential equations was used by SIMPLEST algorithm. The CFD code coupled with Poisson's electric field, ion transport equations and the momentum equation with electric body force, was used for the numerical simulation with the Chen-Kim k-epsiv turbulent model. The numerical results show that EHD secondary flow was clearly visible in the downstream regions of the corona wire despite the low Reynolds number for the electrode (Re _cw=12.4). Secondary flow vortices caused by the EHD increases with increasing discharge current or EHD number, hence pressure drop of ESP increases     

Phase Transformations in Dicalcium Silicate: II, TEM Studies of Crystallography, Microstructure, and Mechanisms

The crystallography, microstructures, and phase transformation mechanisms in dicalcium silicate (Ca₂SiO₄) were studied by TEM. Three types of superlattice structures were observed in the α'_L and β phases. Almost all β grains were twinned and strained. Symmetry-related domain structures inherited from previous high-temperature transformations were observed in β grains. Both the α→α'_H and α'_L→β transformations were considered to be ferroelastic, and spontaneous strains were calculated. In terms of the crystal structures, the major driving force for the β→γ transformation is proposed to be strains and cation charge repulsions in the β structure. This mechanism can be displacive, but it needs to overcome a comparatively high energy barrier.     

Correlated response of udder dimensions to selection for milk yield in Holsteins

Response to selection for milk yield in Holsteins was examined by a controlled selection experiment initiated in 1964. Foundation cows were paired by sire and divided randomly into two breeding groups, selection and control. Selection group was mated to four sires each year highest for Predicted Difference milk. Twenty bulls selected in 1964 as near breed average for milk sired all control cows. Milk yield was recorded for each lactation, and height of udder from ground, distances between teats, and perimeter and area bound by the four teats were measured at 30 to 75 days postpartum on 153 selection and 202 control cows over 14 yr. Most estimates of repeatability and heritability of udder dimensions before and after milking were .45 or larger. Correlations with milk yield were negative for udder height and positive for other udder measurements. Selection cows exceeded controls for lactational milk yield. Daughters of high milk bulls had greater distances between teats, greater perimeters, and larger areas of udder floor. Selection cows did not differ from controls in udder height for first parity but were below controls in udder height and had udders that collapsed more for all parities.