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201.
A direct, rapid, and easy electrospray ionization mass spectrometry (ESI-MS) method to determine concentrations of sebacic acid (SA) and terephthalic acid (TA) residues in biodegradable copolymers was developed. Copolyester samples were synthesized from 1,4-butanediol and sebacic and terephthalic acids by melt polymerization. Extraction of monomers was performed in methanol. Their concentrations were determined by direct infusion ESI-MS, without chromatographic separation, using 1,12-dodecanedioic acid (DDA) as an internal standard. Calibration curves were obtained by plotting the ratio of the areas of the peaks relative to monomers and DDA standard as a function of their concentration ratio. We validated the method by determining the concentration of TA residue using both the ESI-MS protocol and high-performance liquid chromatography (HPLC) analysis with UV detection. The linearity range and the detection limit of this assay were 0.1-5.0 and 0.01 ppm for SA and 0.1-6.0 and 0.03 ppm for TA. This assay represents a useful alternative to conventional methods currently employed for acid quantification, resulting advantageous for its speed and high sensitivity. 相似文献
202.
Daniela K?nnicke Alexandra Kühn Thorsten Mahrholz Michael Sinapius 《Journal of Materials Science》2011,46(21):7046-7055
Nanoscale aluminium trihydroxide (ATH) is examined to its suitability for carbon fibre reinforced plastics (CFRP) as a novel
flame retardant. In particular the influence of particle size and concentration of ATH to the fire behaviour of epoxy polymer
matrices is determined. The particle size is adjusted by means of different dispersing techniques. By SEM images and viscosity
measurements the homogeneity of the produced ATH dispersions for the liquid ones and of cured epoxy–ATH nanocomposites is
determined. Based on these pre-results, selected ATH dispersions are used for the manufacturing of CFRP which are produced
by the proved injection technology. The thermal stability of the ATH nanocomposite plates and the corresponding CFRP plates
are analyzed by means of quantitative single difference thermoanalysis (QSDTA). The fire behaviour is characterised by using
the OSU chamber test. Both tests indicate a reduced heat release rate by decreasing the ATH particle size, i.e. the thermal
load decreases. A combined fire protection mechanism is discussed for the improved fire protection through ATH nanoparticles. 相似文献
203.
Vittoria Bruni Daniela De Canditiis Domenico Vitulano 《Signal, Image and Video Processing》2014,8(7):1199-1209
This paper presents SUVEHP (speed up of video enhancement based on human perception), a human perception-based model oriented to reduce the computational time of digital video restoration. In particular, two specific hypothesis tests able to classify degraded frame regions are proposed. Classification is performed in agreement with regions visual significance in order to enable or inhibit motion compensated enhancement. The level of the proposed hypothesis tests is theoretically assessed. Moreover, extensive experimental results on video sequences affected by additive Gaussian noise show that SUVEHP speeds up some standard motion compensated denoisers up to 60%, preserving or even slightly increasing both the objective and subjective visual quality of the restored sequences. 相似文献
204.
Antonio Matera Alessio Marella Akihiro Maeda Matteo C. Da Vi Francesca Lazzaroni Sonia Fabris Stefania Pioggia Laura Porretti Federico Colombo Federica Torricelli Antonino Neri Elisa Taiana Giuseppina Fabbiano Valentina Traini Elisa Genuardi Daniela Drandi Niccol Bolli Marta Lionetti 《International journal of molecular sciences》2022,23(24)
Multiple myeloma (MM) has a highly heterogeneous genetic background, which complicates its molecular tracking over time. Nevertheless, each MM patient’s malignant plasma cells (PCs) share unique V(D)J rearranged sequences at immunoglobulin loci, which represent ideal disease biomarkers. Because the tumor-specific V(D)J sequence is highly expressed in bulk RNA in MM patients, we wondered whether it can be identified by single-cell RNA sequencing (scRNA-seq). To this end we analyzed CD138+ cells purified from bone marrow aspirates of 19 samples with PC dyscrasias by both a standard method based on bulk DNA and by an implementation of the standard 10x Genomics protocol to detect expressed V(D)J sequences. A dominant clonotype was easily identified in each sample, accounting on average for 83.65% of V(D)J-rearranged cells. Compared with standard methods, scRNA-seq analysis proved highly concordant and even more effective in identifying clonal productive rearrangements, by-passing limitations related to the misannealing of consensus primers in hypermutated regions. We next validated its accuracy to track 5 clonal cells with absolute sensitivity in a virtual sample containing 3180 polyclonal cells. This shows that single-cell V(D)J analysis may be used to find rare clonal cells, laying the foundations for functional single-cell dissection of minimal residual disease. 相似文献
205.
Carmelina Daniela Anfuso Alessia Cosentino Aleksandra Agafonova Agata Zappal Giovanni Giurdanella Angela Trovato Salinaro Vittorio Calabrese Gabriella Lupo 《International journal of molecular sciences》2022,23(24)
The stria vascularis (SV) contributes to cochlear homeostasis and consists of three layers, one of which contains the blood-labyrinthic barrier (BLB), with a large number of bovine cochlear pericytes (BCPs). Cisplatin is a chemotherapeutic drug that can damage the SV and cause hearing loss. In this study, cell viability, proliferation rate, cytotoxicity and reactive oxygen species production were evaluated. The protein content of phospho-extracellular signal-regulated kinases (ERK) 1/2, total ERK 1/2, phospho-cytosolic phospholipase A2 (cPLA2), total cPLA2 and cyclooxygenase 2 (COX-2) and the release of prostaglandin E2 (PGE2) and vascular endothelial growth factor (VEGF) from BCPs were analyzed. Finally, the protective effect of platelet-derived growth factor (PDGF-BB) on BCPs treated with cisplatin was investigated. Cisplatin reduced viability and proliferation, activated ERK 1/2, cPLA2 and COX-2 expression and increased PGE2 and VEGF release; these effects were reversed by Dexamethasone. The presence of PDGF-BB during the treatment with cisplatin significantly increased the proliferation rate. No studies on cell regeneration in ear tissue evaluated the effect of the PDGF/Dex combination. The aim of this study was to investigate the effects of cisplatin on cochlear pericytes and propose new otoprotective agents aimed at preventing the reduction of their vitality and thus maintaining the BLB structure. 相似文献
206.
Luca Mignani Daniela Zizioli Deepak Khatri Nicola Facchinello Marco Schiavone Giuseppe De Palma Dario Finazzi 《International journal of molecular sciences》2022,23(21)
Coenzyme A (CoA) is an essential cofactor in all living organisms, being involved in a large number of chemical reactions. Sequence variations in pantothenate kinase 2 (PANK2), the first enzyme of CoA biosynthesis, are found in patients affected by Pantothenate Kinase Associated Neurodegeneration (PKAN), one of the most common forms of neurodegeneration, with brain iron accumulation. Knowledge about the biochemical and molecular features of this disorder has increased a lot in recent years. Nonetheless, the main culprit of the pathology is not well defined, and no treatment option is available yet. In order to contribute to the understanding of this disease and facilitate the search for therapies, we explored the potential of the zebrafish animal model and generated lines carrying biallelic mutations in the pank2 gene. The phenotypic characterization of pank2-mutant embryos revealed anomalies in the development of venous vascular structures and germ cells. Adult fish showed testicular atrophy and altered behavioral response in an anxiety test but no evident signs of neurodegeneration. The study suggests that selected cell and tissue types show a higher vulnerability to pank2 deficiency in zebrafish. Deciphering the biological basis of this phenomenon could provide relevant clues for better understanding and treating PKAN. 相似文献
207.
Graziano Colombo Alessandra Altomare Emanuela Astori Lucia Landoni Maria Lisa Garavaglia Ranieri Rossi Daniela Giustarini Maria Chiara Lionetti Nicoletta Gagliano Aldo Milzani Isabella Dalle-Donne 《International journal of molecular sciences》2023,24(1)
Urea is the uremic toxin accumulating with the highest concentration in the plasma of chronic kidney disease (CKD) patients, not being completely cleared by dialysis. Urea accumulation is reported to exert direct and indirect side effects on the gastrointestinal tract, kidneys, adipocytes, and cardiovascular system (CVS), although its pathogenicity is still questioned since studies evaluating its side effects lack homogeneity. Here, we investigated the effects of physiological and pathological urea concentrations on a human endothelial cell line from the microcirculation (Human Microvascular Endothelial Cells-1, HMEC-1). Urea (5 g/L) caused a reduction in the proliferation rate after 72 h of exposure and appeared to be a potential endothelial-to-mesenchymal transition (EndMT) stimulus. Moreover, urea induced actin filament rearrangement, a significant increase in matrix metalloproteinases 2 (MMP-2) expression in the medium, and a significant up- or down-regulation of other EndMT biomarkers (keratin, fibrillin-2, and collagen IV), as highlighted by differential proteomic analysis. Among proteins whose expression was found to be significantly dysregulated following exposure of HMEC-1 to urea, dimethylarginine dimethylaminohydrolase (DDAH) and vasorin turned out to be down-regulated. Both proteins have been directly linked to cardiovascular diseases (CVD) by in vitro and in vivo studies. Future experiments will be needed to deepen their role and investigate the signaling pathways in which they are involved to clarify the possible link between CKD and CVD. 相似文献
208.
209.
Maria Fernanda Garcs Julieth Daniela Buell-Acosta Edith ngel-Müller Arturo Jos Parada-Baos Jaidy Acosta-Alvarez Harold Felipe Saavedra-Lpez Roberto Franco-Vega Luis Miguel Maldonado-Acosta Franklin Escobar-Cordoba Keydy Vsquez-Romero Ezequiel Lacunza Sofía Alexandra Caminos-Cepeda Rubn Nogueiras Carlos Diguez Ariel Ivn Ruiz-Parra Jorge Eduardo Caminos 《International journal of molecular sciences》2022,23(17)
The Liver-Expressed Antimicrobial Peptide 2 (LEAP-2) has emerged as an endogenous GHS-R antagonist and blunts the orexigenic action of ghrelin. This study aimed to determine the Ghrelin/LEAP-2 ratio in humans and rats during pregnancy. In humans, we conducted a nested case-control study within an observational prospective cohort. Healthy and mild preeclamptic pregnant women were studied at each trimester of gestation and three months postpartum. In addition, a group of non-pregnant women was studied into the follicular and luteal phases of the menstrual cycle. Furthermore, Ghrelin/LEAP-2 ratio was investigated in non-pregnant rats and at different periods of rat pregnancy. Human and rat serum ghrelin and LEAP-2 levels were determined using the commercially available ELISA kits. The Ghrelin/LEAP-2 ratio peak around the second trimester of gestation in healthy pregnant women (p < 0.05). Additionally, there were no statistically significant differences in Ghrelin/LEAP-2 ratio between healthy and preeclamptic pregnant women at each trimester of gestation (p > 0.05). The Ghrelin/LEAP-2 ratio in pregnant rat reached the peak around mid-gestation with a similar pattern to the human pregnancy. LEAP-2 was visualized by immunohistochemistry in human term placenta and rat placentas on days 12, 16 and 21 of pregnancy. In conclusion, this study provides the first evidence of a Ghrelin/LEAP-2 ratio peak around the half-way point of pregnancy onwards during human and rat pregnancy, and it might be associated with increased rates of weight gain during pregnancy. Thus, this study suggests that LEAP-2 and Ghrelin/LEAP-2 ratio might play an important role in maternal physiology adaptation of weight gain during pregnancy. 相似文献
210.
Giovanni Tossetta Sonia Fantone Teresa Lorenzi Andrea Benedetto Galosi Andrea Sagrati Mara Fabri Daniela Marzioni Manrico Morroni 《International journal of molecular sciences》2022,23(18)
Background: The scattered tubular cells (STCs) are a population of resident progenitor tubular cells with expansion, self-renewal and epithelial differentiation abilities. Although these cells are localized within the proximal (PTs) and distal (DTs) tubules in a normal adult kidney, their presence has never been demonstrated in human macula densa (MD). The purpose of the present study is to describe the presence of STCs in MD using specific markers such as prominin-1 (CD133), cytokeratin 7 (KRT7) and vimentin (VIM). Methods: We analyzed two sets of three consecutive serial sections for each sample. The first sections of each set were immunostained for nNOS to identify MD, the second sections were immune-stained for CD133 (specific STCs marker) while the third sections were analyzed for KRT7 (another STCs specific marker) and VIM (that stains the basal pole of the STCs) in the first and second sets, respectively, in order to study the co-expression of KRT7 and VIM with the CD133 marker. Results: CD133 was localized in some MD cells and in the adjacent DT cells. Moreover, CD133 was detected in the parietal epithelial cells of Bowman’s capsule and in some proximal tubules (PT). KRT7-positive cells were identified in MD and adjacent DT cells, while KRT7 positivity was mostly confined in both DT and collecting ducts (CD) in the other areas of the renal parenchyma. CD133 and KRT7 were co-expressed in some MD and adjacent DT cells. Some of the latter cells were positive both for CD133 and VIM. CD133 was always localized in the apical part of the cells, whereas the VIM expression was evident only in the cellular basal pole. Although some cells of MD expressed VIM or CD133, none of them co-expressed VIM and CD133. Conclusions: The presence of STCs was demonstrated in human adult MD, suggesting that this structure has expansion, self-renewal and epithelial differentiation abilities, similar to all other parts of renal tubules. 相似文献