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The wound is induced by several mechanical and metabolic factors. In the etiology of the wound recovery,
excessive oxidative stress, calcium ion (Ca2+) influx, and apoptosis have important roles. Ca2+-permeable TRPM2 channel is activated by oxidative stress. Protective roles of Hypericum perforatum extract (HP) on the mechanical nerve injury-induced apoptosis and oxidative toxicity through regulation of TRPM2 in the experimental animals were
recently reported. The potential protective roles in HP treatment were evaluated on the TRPM2-mediated cellular
oxidative toxicity in the renal epithelium (MPK) cells. The cells were divided into three groups as control, wound,
and wound + HP treatment (75 µM for 72 h). Wound diameters were more importantly decreased in the wound+HP
group than in the wound group. In addition, the results of laser confocal microscopy analyses indicated protective
roles of HP and TRPM2 antagonists (N-(p-Amylcinnamoyl) anthranilic acid and 2-aminoethyl diphenylborinate)
against the wound-induced increase of Ca2+ influx and mitochondrial ROS production. The wound-induced increase
of early (annexin V-FITC) apoptosis and late (propidium iodide) apoptosis were also decreased in the cells by the HP
treatment. In conclusion, HP treatment acted protective effects against wound-mediated oxidative cell toxicity and
apoptosis through TRPM2 inhibition. These effects may be attributed to their potent antioxidant effect. 相似文献
94.
Iliescu Florina Silvia Teo Jeremy Choon Meng Vrtacnik Danilo Taylor Hayden Iliescu Ciprian 《Microsystem Technologies》2018,24(7):2905-2912
Microsystem Technologies - Cell transplantation traditionally employs needles to inject donor cells into tissues to treat certain diseases. However, it is difficult for the current method to... 相似文献
95.
Aristides D. Tagalakis Vignesh Jayarajan Ruhina Maeshima Kin H. Ho Farhatullah Syed Lin-Ping Wu Ahmad M. Aldossary Mustafa M. Munye Talisa Mistry Olumide Kayode Ogunbiyi Arturo Sala Joseph F. Standing Seyed M. Moghimi Andrew W. Stoker Stephen L. Hart 《Advanced functional materials》2021,31(37):2104843
96.
Danilo Marimpietri Irma Airoldi Angelo Corso Faini Fabio Malavasi Fabio Morandi 《International journal of molecular sciences》2021,22(8)
The long-underestimated role of extracellular vesicles in cancer is now reconsidered worldwide by basic and clinical scientists, who recently highlighted novel and crucial activities of these moieties. Extracellular vesicles are now considered as king transporters of specific cargoes, including molecular components of parent cells, thus mediating a wide variety of cellular activities both in normal and neoplastic tissues. Here, we discuss the multifunctional activities and underlying mechanisms of extracellular vesicles in neuroblastoma, the most frequent common extra-cranial tumor in childhood. The ability of extracellular vesicles to cross-talk with different cells in the tumor microenvironment and to modulate an anti-tumor immune response, tumorigenesis, tumor growth, metastasis and drug resistance will be pinpointed in detail. The results obtained on the role of extracellular vesicles may represent a panel of suggestions potentially useful in practice, due to their involvement in the response to chemotherapy, and, moreover, their ability to predict resistance to standard therapies—all issues of clinical relevance. 相似文献
97.
Akin Mustafa Günsel Armağan Bilgiçli Ahmet T. Tüzün Burak Arabaci Gulnur Şaki Neslihan Yarasir M. Nilüfer 《Protection of Metals and Physical Chemistry of Surfaces》2020,56(3):609-618
Protection of Metals and Physical Chemistry of Surfaces - Corrosion inhibition effects of water-soluble peripheral substituted cobalt, copper and zinc metallophthalocyanines (CoPc (1), CuPc (2) and... 相似文献
98.
Korkanç Mustafa İnce İsmail Hatır M. Ergün Tosunlar M. Bahadır 《Bulletin of Engineering Geology and the Environment》2021,80(4):3053-3063
Bulletin of Engineering Geology and the Environment - The aim of this study is to determine the extent of deterioration of the limestone on which the İvriz rock monument is engraved. This... 相似文献
99.
Emanuele Francesco Marano Danilo Lussana Alberto Castellero Marcello Baricco 《Metals and Materials International》2016,22(2):305-310
Nanoporous microtubes of a nickel-copper alloy were obtained from a Cu-44Ni-1Mn (wt%) commercial wire (200 μm diameter). A new synthesis method was established through three steps: 1) partial oxidation of the wire at 1173 K in air, 2) removal of the inner unoxidized core by chemical etching, 3) reduction in 10 bar hydrogen atmosphere. During oxidation, the segregation of Cu and Ni occurred because of their different diffusion coefficients in the corresponding oxides. As a consequence, pores were formed by Kirkendall effect and due to selective chemical etching of the different oxides. Additional porosity formed because of volume contraction during reduction with hydrogen. After reduction, the microtube shows a composition gradient from the inner wall (almost pure nickel) to the outer wall (almost pure copper). The process allowed to obtain microtubes with tuneable wall thickness and inner pores around 180 ± 80 nm. The morphological features developed suggest improved capillarity properties for applications in MEMS. 相似文献
100.
Yunus Emre Unsal Mustafa Soylak Mustafa Tuzen 《International Journal of Food Science & Technology》2012,47(6):1253-1258
A new and simple solid‐phase extraction method for the determination of trace levels of sunset yellow has been developed on Diaion HP 2MG column system. The adsorbed sunset yellow on the resin was eluted with 3 mL of methanol and determined by UV–Vis spectrophotometry at 484 nm. Various experimental parameters such as pH, amount of sunset yellow, flow rates of sample and eluent solutions, resin amount, sample volumes, etc. were investigated. The influences of some alkali, alkali earth and transition metals on the recoveries of sunset yellow were investigated. The recovery values for sunset yellow were higher than 95%. Preconcentration factor was found as 150. The detection limit (three sigma) of the reagent blank for sunset yellow was 5.2 μg L?1. The relative standard deviations (RSD) of the procedure were found as 3.9%. The validation of the presented procedure was performed by the method of standard addition. The procedure was successfully applied to the determination of trace levels of sunset yellow in powdered beverage and confectionery products. 相似文献