Characterization of a new type HPV16 E7 variant isolated from cervical cancer highest incidence area in Hubei Province of China

AIM: To investigate the variation and biological properties of HPV16 E7 isolated from cervical cancer biopsy samples from highest incidence area in HuBei province of China. METHODS: HVP16 E7 sequences isolated from the cervical cancer biopsies of 10 local patients were amplified, sequenced and compared with prototype E7 gene. Then the variant gene was cloned into different vectors to study the antigenicity, expression and immunogenicity of its protein by Western blot, immunofluorescence and genetic immunization in vitro or in vivo. RESULTS: The results showed that 7 of 10 samples had the same mutations which led to a nonsense mutation at codon 43 of E7 sequence. The truncated E7 protein could be recognized by standard E7 monoclonal antibody in Western blot and expressed in NIH3T3 cells. In the blood sera of mice immunized intramuscularly by the plasmid DNA expressing the variant E7 gene specific E7 antibodies could be detected at week 2, 3, 5 and 6 after inoculation. However, no specific lymphoproliferation after E7 protein stimulation in vitro was detected by MTT colorimetric assay in comparison to the prototype E7 protein. CONCLUSION: HPV16 E7 gene may show variation in China and the variant protein could be expressed and induce host humoral immune response, but could not elicit special cellular-immune response against it. These data might hold the key for future development of HPV16 vaccine in HuBei province of China.     

High-power supercontinuum generation in a nested linear cavity involving a CW Raman fiber laser

We construct a novel nested linear cavity configuration to generate supercontinuum (SC) under continuous-wave pump. With this structure, stimulated Raman scattering and four-wave mixing can affect the whole region of the SC spectrum while the pump line is removed outside of the continuum, and a symmetrical spectrum can be obtained. The properties of the structure are demonstrated by using 3.5-km single-mode fiber, and an SC centered at 1315 nm with the bandwith of 102 nm and the output power of 1.3 W is achieved. By tuning the feedback fiber Bragg grating, we can easily adjust the spectral shape of SC to fit different requirement.     

电力宽带的改进措施与拓展应用

随着互联网业务的快速增长,宽带技术应用日益广泛.本文通过对电力载波宽带技术(PLC)的进一步分析,给出了干扰的成因和对抗的措施,研究了通信新技术(如FDM、ATM等)在PLc中的作用,提出了电力宽带的拓展运用模式.     

基于LabVIEW的伽玛能谱仪的设计

目前,绝大多数传统的伽玛能谱仪均是采用专用的多道分析器,整个系统缺乏共享性和灵活性,且价格十分昂贵。为了提高系统的互用性、灵活性,同时最大限度地降低成本,而采用虚拟仪器技术进行伽玛能谱仪的设计与开发。不仅可以充分利用计算机的丰富资源,且使用灵活、界面友好、成本低、易于扩充和修改。     

Fast mode dependent directional transform via butterfly-style transform and integer lifting steps

Mode dependent directional transform (MDDT) can improve the coding efficiency of H.264/AVC but it also brings high computation complexity. In this paper we present a new design for implementing fast MDDT transform through integer lifting steps. We first approximate the optimal MDDT by a proper transform matrix that can be implemented with butterfly-style operation. We further factorize the butterfly-style transform into a series of integer lifting steps to eliminate the need of multiplications. Experimental results show that the proposed fast MDDT can significantly reduce the computation complexity while introducing negligible loss in the coding efficiency. Due to the merit of integer lifting steps, the proposed fast MDDT is reversible and can be implemented on hardware very easily.     

激光修整埋入无源元件的最佳设计

当设计具有埋入无源元件的PCB时,必须仔细考虑影响修整生产率和准确性的因素,而采用激光修整是最佳的。     

Effect of Pulse-Plated Nickel Barriers on Tin Whisker Growth for Pure Tin Solder Joints

We investigate the influence of pulse-plated Ni barriers, compared to direct current (DC)-plated Ni barriers, on the growth of Sn whiskers in laminated Cu/Ni/Sn samples. The results indicate that the pulse-plated Ni barriers exhibit much better resistance to Sn whisker growth than the DC-plated Ni barriers, i.e., when exposed to ambient of 60°C and 93% relative humidity (RH) for 40 days only a few small hillocks were observed as opposed to the long whiskers and large nodules of Sn for the DC-plated Ni barriers. The underlying mechanisms are addressed based on the texture characteristics of the plated Ni and Sn layers and the formation of intermetallic compounds.     

Alcohol soluble titanium(IV) oxide bis(2,4-pentanedionate) as electron collection layer for efficient inverted polymer solar cells

We demonstrate efficient inverted polymer solar cells (PSCs) based on poly(3-hexylthiophene) (P3HT) and [6,6]-phenyl-C₆₁-butyric acid methyl ester (PCBM) by using solution-processed titanium(IV) oxide bis(2,4-pentanedionate) (TOPD) as electron collection layer (ECL) between the indium tin oxide (ITO) electrode and photoactive layer. The TOPD buffer layer was prepared by spin-coating isopropanol solution of TOPD on ITO and then baked at 140 °C for 5 min. The power conversion efficiency (PCE) of the inverted PSC with TOPD buffer layer reaches 4% under the illumination of AM1.5G, 100 mW/cm², which is increased by 76% in comparison with that (2.27%) of the inverted device without TOPD ECL. The results indicate that TOPD is a promising electron collection layer for inverted PSCs.     

同时实现OR及XOR逻辑运算的多功能计算全息光学元件

本文提出了一种在光学上同时实现两图像间的OR及XOR运算的方法,利用计算全息制成了同时进行上述两种基本逻辑运算的光学元件,并给出了实验结果。     

基于Nexperia PNX1500的H.264视频编码的实现和优化

本文介绍低码率视频编码国际标准H.264,重点探讨H.264的编码在DSPNexperiaPNX1500上如何实现和进行优化。