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151.
I Santamaría G Velasco M Cazorla A Fueyo E Campo C López-Otín 《Canadian Metallurgical Quarterly》1998,58(8):1624-1630
We have identified and cloned a new member of the papain family of cysteine proteinases from a human brain cDNA library. The isolated cDNA codes for a polypeptide of 334 amino acids that exhibits all of the structural features characteristic of cysteine proteinases, including the active site cysteine residue essential for peptide hydrolysis. Pairwise comparisons of this amino acid sequence with the remaining human cysteine proteinases identified to date showed a high percentage of identity (78%) with cathepsin L; the percentage of identity with all other members of the family was much lower (<40%). On the basis of these structural characteristics, we have tentatively called this novel protein cathepsin L2. The cDNA encoding the mature cathepsin L2 was expressed in Escherichia coli, and after purification, the recombinant protein was able to degrade the synthetic peptide benzyloxycarbonyl-L-phenylalanyl-L-arginine-7-amido-4-methylcoumarin, which is commonly used as a substrate for cysteine proteinases. Cathepsin L2 proteolytic activity on this substrate was abolished by trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, an inhibitor of cysteine proteinases, thus providing additional evidence that the isolated cDNA encodes a functional cysteine proteinase. Northern blot analysis of polyadenylated RNAs isolated from a variety of human tissues demonstrated that cathepsin L2 is predominantly expressed in the thymus and testis. This finding is in marked contrast with the wide tissue distribution of most cysteine proteinases characterized to date, including cathepsin L, and suggests that cathepsin L2 may play a specialized role in the thymus and testis. Expression analysis of cathepsin L2 in human tumors revealed a widespread expression in colorectal and breast carcinomas but not in normal colon or mammary gland or in peritumoral tissues. Cathepsin L2 was also expressed by colorectal and breast cancer cell lines as well as by some tumors of diverse origin, including ovarian and renal carcinomas. These results open the possibility that this novel enzyme may be involved in tumor processes, as already reported for other cysteine proteinases, including cathepsin L. 相似文献
152.
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154.
Stefan K. Gehrig Hernán Badino Uwe Franke 《Computer Vision and Image Understanding》2012,116(1):16-24
Dense stereo algorithms are able to estimate disparities at all pixels including untextured regions. Typically these disparities are evaluated at integer disparity steps. A subsequent sub-pixel interpolation often fails to propagate smoothness constraints on a sub-pixel level.We propose to increase the sub-pixel accuracy in low-textured regions in four possible ways: First, we present an analysis that shows the benefit of evaluating the disparity space at fractional disparities. Second, we introduce a new disparity smoothing algorithm that preserves depth discontinuities and enforces smoothness on a sub-pixel level. Third, we present a novel stereo constraint (gravitational constraint) that assumes sorted disparity values in vertical direction and guides global algorithms to reduce false matches, especially in low-textured regions. Finally, we show how image sequence analysis improves stereo accuracy without explicitly performing tracking. Our goal in this work is to obtain an accurate 3D reconstruction. Large-scale 3D reconstruction will benefit heavily from these sub-pixel refinements.Results based on semi-global matching, obtained with the above mentioned algorithmic extensions are shown for the Middlebury stereo ground truth data sets. The presented improvements, called ImproveSubPix, turn out to be one of the top-performing algorithms when evaluating the set on a sub-pixel level while being computationally efficient. Additional results are presented for urban scenes. The four improvements are independent of the underlying type of stereo algorithm. 相似文献
155.
Jose Saldana Julián Fernández-Navajas José Ruiz-Mas Jenifer Murillo Eduardo Viruete Navarro José I. Aznar 《Computer Networks》2012,56(7):1893-1919
This work presents a study of RTP multiplexing schemes, which are compared with the normal use of RTP, in terms of experienced quality. Bandwidth saving, latency and packet loss for different options are studied, and some tests of Voice over IP (VoIP) traffic are carried out in order to compare the quality obtained using different implementations of the router buffer. Voice quality is calculated using ITU R-factor, which is a widely accepted quality estimator. The tests show the bandwidth savings of multiplexing, and also the importance of packet size for certain buffers, as latency and packet loss may be affected. The customer’s experience improvement is measured, showing that the use of multiplexing can be interesting in some scenarios, like an enterprise with different offices connected via the Internet. The system is also tested using different numbers of samples per packet, and the distribution of the flows into different tunnels is found to be an important factor in order to achieve an optimal perceived quality for each kind of buffer. Grouping all the flows into a single tunnel will not always be the best solution, as the increase of the number of flows does not improve bandwidth efficiency indefinitely. If the buffer penalizes big packets, it will be better to group the flows into a number of tunnels. The router processing capacity has to be taken into account too, as the limit of packets per second it can manage must not be exceeded. The obtained results show that multiplexing is a good way to improve customer’s experience of VoIP in scenarios where many RTP flows share the same path. 相似文献
156.
In this paper, a new approximation to off-line signature verification is proposed based on two-class classifiers using an
expert decisions ensemble. Different methods to extract sets of local and a global features from the target sample are detailed.
Also a normalization by confidence voting method is used in order to decrease the final equal error rate (EER). Each set of
features is processed by a single expert, and on the other approach proposed, the decisions of the individual classifiers
are combined using weighted votes. Experimental results are given using a subcorpus of the large MCYT signature database for
random and skilled forgeries. The results show that the weighted combination outperforms the individual classifiers significantly.
The best EER obtained were 6.3 % in the case of skilled forgeries and 2.31 % in the case of random forgeries. 相似文献
157.
Julián Luengo José A. Sáez Francisco Herrera 《Soft Computing - A Fusion of Foundations, Methodologies and Applications》2012,16(5):863-881
Fuzzy rule-based classification systems (FRBCSs) are known due to their ability to treat with low quality data and obtain
good results in these scenarios. However, their application in problems with missing data are uncommon while in real-life
data, information is frequently incomplete in data mining, caused by the presence of missing values in attributes. Several
schemes have been studied to overcome the drawbacks produced by missing values in data mining tasks; one of the most well
known is based on preprocessing, formerly known as imputation. In this work, we focus on FRBCSs considering 14 different approaches
to missing attribute values treatment that are presented and analyzed. The analysis involves three different methods, in which
we distinguish between Mamdani and TSK models. From the obtained results, the convenience of using imputation methods for
FRBCSs with missing values is stated. The analysis suggests that each type behaves differently while the use of determined
missing values imputation methods could improve the accuracy obtained for these methods. Thus, the use of particular imputation
methods conditioned to the type of FRBCSs is required. 相似文献
158.
The ability of Lactobacillus jensenii and Streptococcus 17SB to inbhibit Escherichia coli was demonstrated. The practical application of the inhibition was examined. Possible roles for several factors in the mechanism of inhibition by Lactobacillus jensenii and Streptococcus 17SB are briefly discussed. The effects of pH reached and acids produced by starters on the growth of Escherichia coli are described. 相似文献
159.
Federico Ferreres Alberto Ortiz Carmen Silva Christina Garcia-Viguera Francisco A. Tomás-Barberán Francisco Tomás-Lorente 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1992,194(2):139-143
Summary The flavonoids present in ten selected samples of La Alcarria honey with different pollen compositions have been HPLC analysed in order to establish if correlations between botanical origin and flavonoid profiles are possible. A common flavonoid pattern is observed in the different samples showing that pollen is not the main source of honey flavonoids. A close correlation between the flavonoid patterns of honey flavonoids and propolis flavonoids has been found suggesting that flavonoid analysis could be more useful in geographical origin determinations than in botanical origin studies.
Flavonoide des La-Alcarria-Honigs Eine Studie ihres botanischen Ursprungs
Zusammenfassung Die Flavonoide in Proben in Alcarria-Honig mit unterschiedlicher Pollenzusammensetzung wurden untersucht, um Kortrelationen zwischen dem botanischen Ursprung und den möglichen Flavonoiden zu finden. In den verschiedenen Proben wurde ein Flavonoid-Muster gefunden, wobei der Pollen nicht die Hauptquelle der Honigflavonoide ist. Es wurde jedoch eine enge Korrelation zwischen den Flavonoid-Mustern des Honigs und des Bienenkittharzes gefunden, was für die geographische Herkunft wichtiger ist als die botanische.相似文献
160.
G. Monrós J. Carda M. A. Tena P. Escribano J. Alarcón 《Journal of Materials Science》1992,27(2):351-357
The preparation of V-ZrSiO4 solid solutions starting from different ZrO2 precursors by using sol-gel methods is reported. The starting materials were hydrolysed and the dried gels were fired at a temperature between 500 and 900 °C with soaking times of 12h. The organic character of zirconia precursors was stronger, i.e. the starting material had more carbon atoms, a higher temperature was necessary to make the first crystalline phase appear (ZrO2(tetragonal)) and the temperature range for the whole phase transformation was narrower. In all dried gel samples the presence of infrared bands which might be associated with either Si-O-Zr or Si-O-V was not observed. On the other hand, some bands could be attributed to a silica network and ZrO8 groups. The main steps in V-ZrSiO4 solid solutions were confirmed. ZrO2(tetragonal) is crystallized on heating from an amorphous sample. The ZrO2(tetragonal) ZrO2(monoclinic) phase transformation then occurs and immediately afterwards the zircon formation begins. Finer textures in samples were obtained from polymeric gels rather than for colloidal gel samples, as seen from the scanning electron micrographs. 相似文献