Properties of mixed chlorophyll a-glycolipid monolayers

Surface pressure, ellipsometric and surface potential-area isotherms have been measured to investigate the properties of chlorophyll a-digalactosyldiacyglycerol (Chl a-DGDG) and Chl a-sulfoquinovosyldiacylglycerol (SQDG) monolayers at the nitrogen-water interface. The surface pressure-area isotherms of Chla-DGDG exhibit a pronounced negative deviation with respect to ideality, whereas ellipsometric and surface potential isotherms of the mixed monolayers fall between those of the pure components. Hydrophilic interactions have been ruled out on the basis of the additivity law followed by the surface dipole moment of the mixed monolayers. The observed deviation exhibited by the π-A isotherms is interpreted in terms of an intermolecular cavity effect.     

1.5%-/spl Delta/ athermal arrayed-waveguide grating multi/demultiplexer with very low loss groove design

We describe a compact 1.5%-/spl Delta/ athermal silica-based 100-GHz-spacing 16-channel arrayed-waveguide grating (AWG) multi/demultiplexer with a modified groove design for a very low excess loss. We propose new approaches for modifying the shape of the grooves and the arrayed waveguides and optimize the shape modifications for 1.5%-/spl Delta/ waveguides. By employing this modified groove design, we greatly reduced the groove excess loss from 1.9 to 0.4 dB in the 1.5%-/spl Delta/ athermal AWG.     

Synthesis of silica-coated rhodium nanoparticles in reversed micellar solution

Silica (SiO₂)-coated rhodium (Rh) nanoparticles were prepared using a water-in-oil microemulsion of polyoxyethylene (15) cetyl ether, cyclohexane and water. SiO₂-coated Rh nanoparticles were obtained by hydrolyzing metal alkoxide (tetraethylorthosilicate, TEOS) in the solution containing Rh complex nanoparticles followed by thermal and reduction treatments. In the SiO₂-coated Rh nanoparticle, a Rh particle with an average diameter of 4.1 nm was located nearly at the center of each spherical SiO₂ particle. The SiO₂ layer was approximately 15 nm thick. Since the Rh particle was wholly surrounded by SiO₂, the Rh particle of the SiO₂-coated Rh nanoparticle exhibited an extremely high thermal stability. Furthermore, the porous structure of the SiO₂ layer could be controlled by the hydrolysis conditions of TEOS.     

Relationship between Expression of Onco-Related miRNAs and the Endoscopic Appearance of Colorectal Tumors

Accumulating data indicates that certain microRNAs (miRNAs or miRs) are differently expressed in samples of tumors and paired non-tumorous samples taken from the same patients with colorectal tumors. We examined the expression of onco-related miRNAs in 131 sporadic exophytic adenomas or early cancers and in 52 sporadic flat elevated adenomas or early cancers to clarify the relationship between the expression of the miRNAs and the endoscopic morphological appearance of the colorectal tumors. The expression levels of miR-143, -145, and -34a were significantly reduced in most of the exophytic tumors compared with those in the flat elevated ones. In type 2 cancers, the miRNA expression profile was very similar to that of the exophytic tumors. The expression levels of miR-7 and -21 were significantly up-regulated in some flat elevated adenomas compared with those in exophytic adenomas. In contrast, in most of the miR-143 and -145 down-regulated cases of the adenoma-carcinoma sequence and in some of the de novo types of carcinoma, the up-regulation of oncogenic miR-7 and/or -21 contributed to the triggering mechanism leading to the carcinogenetic process. These findings indicated that the expression of onco-related miRNA was associated with the morphological appearance of colorectal tumors.     

Structural Analysis of the Complex between Penta-EF-Hand ALG-2 Protein and Sec31A Peptide Reveals a Novel Target Recognition Mechanism of ALG-2

ALG-2, a 22-kDa penta-EF-hand protein, is involved in cell death, signal transduction, membrane trafficking, etc., by interacting with various proteins in mammalian cells in a Ca²⁺-dependent manner. Most known ALG-2-interacting proteins contain proline-rich regions in which either PPYPXnYP (type 1 motif) or PXPGF (type 2 motif) is commonly found. Previous X-ray crystal structural analysis of the complex between ALG-2 and an ALIX peptide revealed that the peptide binds to the two hydrophobic pockets. In the present study, we resolved the crystal structure of the complex between ALG-2 and a peptide of Sec31A (outer shell component of coat complex II, COPII; containing the type 2 motif) and found that the peptide binds to the third hydrophobic pocket (Pocket 3). While amino acid substitution of Phe⁸⁵, a Pocket 3 residue, with Ala abrogated the interaction with Sec31A, it did not affect the interaction with ALIX. On the other hand, amino acid substitution of Tyr¹⁸⁰, a Pocket 1 residue, with Ala caused loss of binding to ALIX, but maintained binding to Sec31A. We conclude that ALG-2 recognizes two types of motifs at different hydrophobic surfaces. Furthermore, based on the results of serial mutational analysis of the ALG-2-binding sites in Sec31A, the type 2 motif was newly defined.     

A hybrid approach to keyframe extraction from motion capture data using curve simplification and principal component analysis

In this paper, we propose a novel method to extract keyframes from motion capture data. A hybrid approach, which combines a curve‐simplification algorithm with an initialization procedure including principal component analysis, is adopted. The developed method automatically extracts an appropriate number of keyframes at high speed without performance degradation. Experimental results prove the effectiveness of the present method. © 2014 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.     

STEAM DRYING OF SINTERED SPHERES OF GLASS BEADS

ABSTRACT

Superheated steam drying of sintered spheres of glass beads with different diameters is investigated to reveal the effects of the gravitational force on drying rates. In a previous study, the drying rate curves of small samples with coarse glass beads in which the frictional resistance to flow of water and the effect of the gravitational force are negligible, were predicted by an evaporation zone model.In the present investigation, the drying rate curves of sintered spheres of glass beads with diameters ranging from 1.53×10^-2m to 4.99×lO^-2m are experimentally and theoretically obtained, and also the capillary pressure curves are measured by use of Haines' apparatus. The drying rate curves of large samples in which the effect of the gravitational force can not be negligible, are compared with those for small samples and the difference among these curves is discussed in terms of the moisture distributions which are estimated from the evaporation zone model with the observed capillary pressure curves.     

Magnetic properties of metal layer deposited by reduction of metal ions contained in polymer with applying magnetic field

Metal layer was deposited by the reduction of NiCl₂ and CoCl₂ in polyacrylonitrile film after applying an external magnetic field in directions parallel and perpendicular to the surface of the film; the magnetic properties of the metal-deposited film were investigated. When the parallel magnetic field was applied, the values of coercivity (H_c), remanent flux density (B_r) and maximum flux density (B_s) increased regardless of the composition of the metal ions, compared with the case without the application of the magnetic field. From the scanning electron micrographs and X-ray diffraction analyses, it was shown that the growth of crystalline orientation in the deposited metals was enhanced by applying a parallel magnetic field.     

Simulation of transverse crack formation on continuously cast peritectic medium carbon steel slabs

Reliable data are limited to the critical strain for the formation of transverse cracks on the slabs, owing to experimental difficulty to simulate temperature gradient in solidified shell in continuous casting mold. The present study is to determine the critical strain, ?_c, for the formation of transverse cracks on continuously cast slabs. A convenient and simple hot tensile test using rectangular test pieces with either V-notch or semi-circle notch or oscillation marks has been developed by placing the specimen under similar temperature gradient to that in solidified shell in the mold. The ?_c has been determined at a better accuracy and reproducibility, and the ?_c at a strain rate of 5?10^?4s^?1 is found to be a high 35% for test pieces without notch. It sharply decreases, however, to 10% for those with V- and semi-circle-notches, slightly decreases with increasing notch depth, and further decreases for those with oscillation marks that accompany solute segregation. Reduction of the oscillation mark depth is shown to be important measure to prevent the occurrence of transverse cracking of continuously cast slabs.     

Use of multivariate analysis for the improvement in prediction accuracy of bacterial aerobic plate count by flow cytometry

Flow cytometry (FCM) and aerobic plate count (APC) by the culture method were performed on green tea samples spiked with Escherichia coli type strain NCTC9001 (ATCC11775) solutions of different concentrations. In FCM, fluorescence signals from multiple stained bacteria and other fluorophores are detected using detector channels, and recorded as events with a voltage at each channel. FCM data were analyzed in two ways: conventional and multivariate analysis. In the former, the number of events with voltages larger than the defined threshold values was regarded as the predicted APC. In the latter, voltage histograms of all channels were obtained and merged horizontally to serve as explanatory variables. Then a partial least squares regression (PLSR) model was built to predict APC from the histogram data. The coefficient of determination (R²) and the root mean square error (RMSE) between APC by the culture method and that predicted by conventional FCM were 0.916 and 1.08 cfu/ml². The APC values predicted by the PLSR model and those measured were in good agreement with R² of 0.982 and RMSE of 0.417 cfu/ml, which verified the potential of the proposed method for improving APC prediction accuracy by FCM.