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51.
都汶高速公路庙子坪岷江特大桥震后5号主墩加固技术 总被引:2,自引:0,他引:2
都汶高速公路是成都通往汶川的重要的交通枢纽,为国道213和317合线。都汶高速公路庙子坪岷江特大桥(又名紫坪铺大桥)直接跨越紫坪铺水库和岷江,都江堰端接著名的董家山高瓦斯隧道,汶川端接龙洞子隧道。5.12汶川大地震对庙子坪岷江特大桥产生了不少震害,地震后大桥的加固时间紧、工作量大、技术复杂,这在中国乃至世界桥梁史上前所未有;特别是5号主墩加固异常复杂和艰难,从桥面防撞护栏顶面到5号主墩地面承台顶面之间总体落差114.488 m,5号主墩地面承台顶面到水面水深达65 m。通过对5号主墩承台顶面以上14 m加固区间(全部位于深水区域)的潜水堵孔和钢沉箱工艺进行深入细致的研究,制定了系列相应措施。 相似文献
52.
为了满足明渠水流入流的边界条件,扩展SPH方法在明渠恒定流模拟中的应用,本文建立SPH方法的出入流模型,通过设置相应的出入流边界条件,研究不同坡度下消力池内陡坡水跃特性。针对两种不同流量,对斜坡坡角θ分别在20°、30°、45°条件下的陡坡水跃进行数值模拟。模拟结果与文献中的实验结果进行对比分析,结果表明:出入流模型能为陡坡水跃的数值模拟提供稳定的水流。各陡坡不同流量下水面线的变化趋势基本相同,误差均在±10%内;相同坡角时,单宽流量为0.063 m~2/s的消能效果均大于单宽流量为0.105 m~2/s的消能效果。该方法处理出入流边界简单,合理准确,能够较好地模拟不同坡度下消力池内陡坡水跃,得到陡坡水跃特性,具有一定的可行性和可靠性。 相似文献
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54.
Saccharomyces cerevisiae Rad51 protein is the paradigm for eukaryotic ATP-dependent DNA strand exchange proteins. To explain some of the unique characteristics of DNA strand exchange promoted by Rad51 protein, when compared with its prokaryotic homologue the Escherichia coli RecA protein, we analyzed the DNA binding properties of the Rad51 protein. Rad51 protein binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) in an ATP- and Mg2+-dependent manner, over a wide range of pH, with an apparent binding stoichiometry of approximately 1 protein monomer per 4 (+/-1) nucleotides or base pairs, respectively. Only dATP and adenosine 5'-gamma-(thiotriphosphate) (ATPgammaS) can substitute for ATP, but binding in the presence of ATPgammaS requires more than a 5-fold stoichiometric excess of protein. Without nucleotide cofactor, Rad51 protein binds both ssDNA and dsDNA but only at pH values lower than 6.8; in this case, the apparent binding stoichiometry covers the range of 1 protein monomer per 6-9 nucleotides or base pairs. Therefore, Rad51 protein displays two distinct modes of DNA binding. These binding modes are not inter-convertible; however, their initial selection is governed by ATP binding. On the basis of these DNA binding properties, we conclude that the main reason for the low efficiency of the DNA strand exchange promoted by Rad51 protein in vitro is its enhanced dsDNA-binding ability, which inhibits both the presynaptic and synaptic phases of the DNA strand exchange reaction as follows: during presynapsis, Rad51 protein interacts with and stabilizes secondary structures in ssDNA thereby inhibiting formation of a contiguous nucleoprotein filament; during synapsis, Rad51 protein inactivates the homologous dsDNA partner by directly binding to it. 相似文献
55.
The concept of transcultural nursing is relatively new to the nursing literature. It had been less than 30 years since Madeleine Leininger first began to develop a theory of transcultural nursing as part of a doctoral study in anthropology. Much has changed in that time, and nursing staff development and inservice educators need to provide educational offerings within a multicultural context in a timely manner. Cultural diversity is the standard in the mid-1990s, and those nursing staff development programs that are sensitive to this fact produce employees with advantages over those from settings that do not prepare staff for practice in a constantly changing world. This annotated bibliography about transcultural nursing details key references for staff development and inservice programs. It is not intended as an exhaustive review but rather focuses on the most relevant, timely, and useful of the ever increasing number of publications concerning this important subject. Six major books and four of the most pertinent recent journal articles are included. Conclusions and implications for nursing staff educators are offered. 相似文献
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57.
VG Savchenko EN Parovichnikova VG Isaev IA Demidova RG Kuliev EO Gribanova GA Kliasova RA Kucher AN Sokolov KS Momotiuk IuV Ol''shanskaia NG Tiurina LIu Tikhonova GM Galstian VM Budianski? EA Belousov VA Lapin SV Voloshin TS Konstaninova LB Filatov ON Porokhina EA Smirnova 《Canadian Metallurgical Quarterly》1998,70(7):5-11
58.
WJ Hoogendijk JS Purba MA Hofman RA de Vos EN Jansen DF Swaab 《Canadian Metallurgical Quarterly》1998,43(12):913-917
BACKGROUND: Depression is frequently encountered in Parkinson's disease (PD). In addition, more than half of the PD patients have a disturbed dexamethasone suppression test, which is associated with increased activity of corticotropin-releasing hormone (CRH) neurons. We recently found an increase in CRH neuron number, CRH-messenger RNA, and vasopressin colocalization in CRH neurons in the paraventricular nucleus (PVN) of depressed patients, which may be involved in the pathogenesis of depression. METHODS: The number of neurons expressing CRH was determined in the PVN of 6 depressed PD patients with a high score (> or = 13) on the Hamilton Depression Rating Scale, 6 nondepressed PD patients, and 6 controls. RESULTS: The three groups did not differ in the number of neurons expressing CRH. CONCLUSIONS: We hypothesize that activation of CRH neurons in the PVN, as we recently observed in idiopathic depression, does not play an essential role in depression in PD. 相似文献
59.
DNA sequences from parts of the 12S, 16S and cytochrome b mitochondrial genes, which totalled 1049 aligned base pairs, were used to estimate the relationships of 49 species of Lacertidae, including representatives of 19 out of the 23 recognized genera and 23 species of the paraphyletic genus Lacerta. These data were used, together with morphological information, to estimate the relationships within the family. Molecular evidence corroborates the monophyletic status of many genera and species groups originally based on morphology. It indicates that Psammodromus forms a clade with Gallotia, which is the sister taxon of all other lacertids. These comprise three units: the primarily Afrotropical armatured group; the largely Oriental Takydromus; and the west Palaearctic Lacerta and its derivatives, Podarcis and Algyroides. Morphology also supports the first three assemblages, but suggests that they are derived from a paraphyletic Lacerta. Within Lacerta and its allies, DNA sequence analysis corroborates the affinity of some members of each of the subgenera Lacerta s. str. and Timon, and of the L. saxicola group. It also supports the relationship of L. monticola, L. bonnali and L. horvathi, and suggests that the L. parva--L. fraasi clade and L. brandli are not related to Psammodromus Gallotia, as morphology indicates, but instead are associated respectively with the L. danfordi and L. saxicola groups. DNA sequence data provide additional evidence that the eastern Arabian 'Lacerta' jayakari and 'L.' cyanura are members of the armatured clade and also sister species. Our analysis supports an origin for present lacertids in west Eurasia. The armatured clade invaded Africa, probably in the mid-Miocene, spreading widely and evolving increasingly xeric-adapted forms, one lineage of which later moved back into the Palaearctic. 'Lacerta' jayakari and 'L.' cyanura are assigned to Omanosaura, Lutz and Mayer 1986. The name Gallotiinae Cano, Baez, Lopez-Jurado & Ortega, 1984 is available for the Gallotia-Psammodromus clade, Eremiainae Shcherbak 1975 for the armatured clade and Lacertinae for Lacerta, Podarcis and Algyroides. Two new subgenera of Lacerta are proposed here: Caucasilacerta for L. saxicola and its allies, and Parvilacerta for L. parva and L. fraasi. 相似文献
60.
1999年以来,我国国民生产总值持续快速稳定增长。表1是这期间国民生产总值增长情况。 相似文献