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941.
942.
Pore architecture of diatom frustules: potential nanostructured membranes for molecular and particle separations 总被引:1,自引:0,他引:1
Losic D Rosengarten G Mitchell JG Voelcker NH 《Journal of nanoscience and nanotechnology》2006,6(4):982-989
Diatoms produce diverse three-dimensional regular silica structures with nanometer to micrometer dimensions and hold considerable promise for biological and biomimetic fabrication of nanostructured materials and devices. In the present work, we describe the ultrastructural characterization of porous structures in diatom biosilica and discuss their potential as membrane filters for diffusion based separations. The frustules of two centric diatom species, Coscinodiscus sp. and Thalassiosira eccentrica, were investigated using scanning electron microscopy and atomic force microscopy. Their morphological features, including pore size, shape, porosity, and pore organization, are described. We observed that although pore organization in frustules of Thalassiosira eccentrica and Coscinodiscus sp. is in reverse order, a striking commonality is the size range of the smallest pores in both species (around 40 nm). The consensus lower pore size suggests that frustule valves have a common function at this size of excluding viruses or other deleterious particles, and the pore size and organization is optimized for this purpose. We suggest and implement an experimental approach to study the potential of diatom frustules for diffusive separation of molecular or nanoparticular components in microfluidic or lab-on-a-chip environments. 相似文献
943.
While numerous studies have examined N dynamics along a river continuum, few have specifically examined the role of biofilms. Nitrogen dynamics and microbial community structure were determined on biofilms at six sites along a 120 km stretch of the lowland Ovens River, South Eastern Australia using artificial substrates. Terminal restriction fragment length polymorphism (T‐RFLP), chlorophyll a and protein analyses were used to assess biofilm microbial community composition. N dynamics was determined on the biofilms using the acetylene (C2H2) block technique and assessing changes in NH, NOx and N2O. Unlike microbial community structure, N dynamics were spatially heterogeneous. Nitrification, determined from the difference in accumulation of NH before and after addition of C2H2, occurred mostly in the upper sites with rates up to 1.4 × 10?5 mol m?2 h?1. The highest rates of denitrification occurred in the mid‐reaches of the river (with rates up to 1 × 10?5 mol m?2 h?1) but denitrification was not detected in the lower reaches. At the very most, only 50% of the observed uptake of NOx by the biofilms following addition of C2H2 could be accounted for by denitrification. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献
944.
Polarity effect in electrovibration for tactile display 总被引:1,自引:0,他引:1
Kaczmarek KA Nammi K Agarwal AK Tyler ME Haase SJ Beebe DJ 《IEEE transactions on bio-medical engineering》2006,53(10):2047-2054
Electrovibration is the tactile sensation of an alternating potential between the human body and a smooth conducing surface when the skin slides over the surface and where the current is too small to stimulate sensory nerves directly. It has been proposed as a high-density tactile display method, for example to display pictographic information to persons who are blind. Previous models for the electrovibration transduction mechanism are based on a parallel-plate capacitor in which the electrostatic force is insensitive to polarity. We present experimental data showing that electrovibratory perceptual sensitivity to positive pulses is less than that for negative or biphasic pulses and propose that this disparity may be due to the asymmetric electrical properties of human skin. We furthermore propose using negative pulses for insulated tactile displays based on electrovibration because their sensory thresholds were found to be more stable than for waveforms incorporating positive pulses. 相似文献
945.
Chemical speciation modeling is a vital tool for assessing the bioavailability of inorganic species, yet significant uncertainties in thermodynamic parameters and model form limit its potential for decision-making. In this paper we present a novel method for the quantification of thermodynamic parameter uncertainty and ionic strength correction model uncertainty using Bayesian Markov Chain Monte Carlo (MCMC) estimation methods. These methods allow for the inclusion of correlation modeling, which has not been present in previous work. The MCMC simulations are used to model a natural river water to determine the uncertainty in the calculated environmental speciation of ethylenediamenetetraacetate, a chelating agent that has attracted considerable environmental interest. The results indicate that incorporating correlation among related thermodynamic parameters into the uncertainty model is necessary to correctly quantify the overall system uncertainty. This result indicates the superiority of MCMC estimation methods overtraditional Monte Carlo methods when available data are used to estimate parameter uncertainty in systems with closely related model parameters. 相似文献
946.
Contrasting effects of dissimilatory iron (III) and arsenic (V) reduction on arsenic retention and transport 总被引:1,自引:0,他引:1
Reduction of arsenate As(V) and As-bearing Fe (hydr)- oxides have been proposed as dominant pathways of As release within soils and aquifers. Here we examine As elution from columns loaded with ferrihydrite-coated sand presorbed with As(V) or As(III) at circumneutral pH upon Fe and/or As reduction; biotic stimulated reduction is then compared to abiotic elution. Columns were inoculated with Shewanella putrefaciens strain CN-32 or Sulfurospirillum barnesii strain SES-3, organisms capable of As (V) and Fe (III) reduction, or Bacillus benzoevorans strain HT-1, an organism capable of As(V) but not Fe(III) reduction. On the basis of equal surface coverages, As(III) elution from abiotic columns exceeded As(V) elution by a factor of 2; thus, As(III) is more readily released from ferrihydrite under the imposed reaction conditions. Biologically mediated Asreduction induced by B. benzoevorans enhances the release of total As relative to As (V) under abiotic conditions. However, under Fe reducing conditions invoked by either S. barnesii or S. putrefaciens, approximately three times more As (V or III) was retained within column solids relative to the abiotic experiments, despite appreciable decreases in surface area due to biotransformation of solid phases. Enhanced As sequestration upon ferrihydrite reduction is consistent with adsorption or incorporation of As into biotransformed solids. Our observations indicate that As retention and release from Fe (hydr)oxide(s) is controlled by complex pathways of Fe biotransformation and that reductive dissolution of As-bearing ferrihydrite can promote As sequestration rather than desorption under conditions examined here. 相似文献
947.
PE Ure?a GA Lamas G Mitchell GC Flaker SC Smith FJ Wackers P McEwan MA Pfeffer 《Canadian Metallurgical Quarterly》1999,33(1):180-185
OBJECTIVES: We assessed the abilities of two methods to measure ejection fraction (EF)-radionuclide ventriculography (RVG) and contrast left ventriculography (Cath-EFa) to predict cardiovascular events. BACKGROUND: Both RVG and Cath-EFa are commonly used methods to measure left ventricular performance and assess prognosis. Their comparative abilities to predict clinical events have not been reported. METHODS: Both RVG EF and Cath-EFa were measured within 16 days of myocardial infarction (MI) in 688 patients. The results were divided into terciles. Prognosis by terciles was assessed for each technique. A multivariate analysis was performed to determine which EF measurement was a better predictor of prognosis. RESULTS: Average RVG-EF was 32%+/-7, while Cath-EFa was 42%+/-10. Both RVG and Cath-EFa were poorly correlated (R=0.42). Event rate declined across terciles with increasing EF for both techniques (events in lowest to highest tercile of Cath-EFa 40.7%, 25.9%, 11.6%, p < 0.001; and RVG-EF 39.9%, 26.1%, 15.6%, p < 0.001). There was concordance of terciles in 303 of 688 patients (44%). When patients in the highest RVG terciles were in the highest Cath-EFa tercile, the event rate was 7%. However, when patients in the highest RVG terciles were in the lowest Cath-EFa tercile, the event rate was 19%. Both Cath-EFa (p < 0.001) and RVG-EF (p < 0.001) were independent predictors of cardiovascular events. CONCLUSIONS: Ejection fraction measured by RVG or during catheterization is a valuable tool in the risk stratification of postinfarct patients. When disagreement is present between clinical impression and measurement by either method, the use of an alternative measurement is warranted and complementary. 相似文献
948.
Four rhesus monkeys (Macaca mulatta), maintained at reduced body weight and restricted food availability, had access to a 0.8-mg/ml cocaine solution and vehicle under a concurrent fixed-ratio (FR) 8 schedule. Over days, the cocaine concentration was reduced (0.57, 0.4, 0.2, 0.1, 0.05, 0.025) and then returned, gradually over days, to 0.8 mg/ml. The ratio value was then varied (to 16, 32, 64, 128, and 8). Food access was unrestricted, and the ratio and the concentration manipulations were then repeated. During food restriction cocaine served as a reinforcer for all monkeys, whereas during free feeding cocaine functioned as a reinforcer for 3 of 4 monkeys; with these monkeys, the dose-response curve obtained under free feeding was shifted to the right of that obtained under food restriction. There were no differences in FR response curves obtained during food restriction and unrestricted feeding. These data suggest that food restriction increases cocaine's reinforcing effects and that the higher the cocaine dose, the greater are the reinforcing effects. A demand curve analysis was completed, and data are discussed in terms of microeconomic principles. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
949.
Aarti Singh Chen Chen Weiguo Liu Wayne Mitchell Bertil Schmidt 《IEEE transactions on information technology in biomedicine》2008,12(2):218-225
Comparative genomics provides a powerful tool for studying evolutionary changes among organisms, helping to identify genes that are conserved among species, as well as genes that give each organism its unique characteristics. However, the huge datasets involved makes this approach impractical on traditional computer architectures leading to prohibitively long runtimes. In this paper, we present a new computational grid architecture based on a hybrid computing model to significantly accelerate comparative genomics applications. The hybrid computing model consists of two types of parallelism: coarse grained and fine grained. The coarse-grained parallelism uses a volunteer computing infrastructure for job distribution, while the fine-grained parallelism uses commodity computer graphics hardware for fast sequence alignment. We present the deployment and evaluation of this approach on our grid test bed for the all-against-all comparison of microbial genomes. The results of this comparison are then used by phenotype--genotype explorer (PheGee). PheGee is a new tool that nominates candidate genes responsible for a given phenotype. 相似文献
950.
Werfel J. Mitchell M. Crutchfield J.P. 《Evolutionary Computation, IEEE Transactions on》2000,4(4):388-393
Coevolution, between a population of candidate solutions and a population of test cases, has received increasing attention as a promising biologically inspired method for improving the performance of evolutionary computation techniques. However, the results of studies of coevolution have been mixed. One of the seemingly more impressive results to date was the improvement via coevolution demonstrated by Juille and Pollack (1998) on evolving cellular automata to perform a classification task. Their study, however, like most other studies on coevolution, did not investigate the mechanisms giving rise to the observed improvements. In this paper, we probe more deeply into the reasons for these observed improvements and present empirical evidence that, in contrast to what was claimed by Juille and Pollack, much of the improvement seen was due to their "resource sharing" technique rather than to coevolution. We also present empirical evidence that resource sharing works, at least in part, by preserving diversity in the population. 相似文献