In vitro displacement by rat serum of adsorbed radiolabeled poloxamer and poloxamine copolymers from model and biodegradable nanospheres

Poloxamer 407 and poloxamine 908 have been used by many research groups to modify the surface of both model latex and biodegradable nanospheres, thereby producing nanospheres that have shown reduced protein adsorption in vitro and extended circulation times in vivo. A potential limitation of such systems is the desorption of the copolymer coating layer. We describe a two-stage process to radiolabel poloxamer 407 and poloxamine 908 that has facilitated an investigation into this potential desorption, in vitro. The first stage of the labeling procedure involved the substitution of the terminal hydroxyl groups in each poly(ethylene oxide) (PEO) chain of poloxamer 407 and poloxamine 908 with an amino group. The aminated copolymers were then radiolabeled with 125Iodine Bolton-Hunter reagent. The efficiency of labeling was calculated to be approximately 20% for the tetramine poloxamine 908 and approximately 33% for the diamine poloxamer 407. Remaining free amino groups were then either acetylated, using acetic anhydride, or left in the free amino form. Covalent linkage of the radiolabel to the copolymer was confirmed by nuclear magnetic resonance (NMR) and infrared (IR) spectroscopy. The stability of the link between radiolabel and copolymer to hydrolysis was also confirmed; <4% loss of radiolabel occurred from poloxamine 908 after incubation in phosphate-buffered saline (PBS) at 37 degrees C for 8 days. The radiolabeled copolymers (with the free amino groups acetylated) were then used in experiments that have given the first direct evidence that adsorbed copolymers can be displaced by serum proteins in significant amounts from the surface of model and biodegradable nanospheres. The displacement was highly dependent on copolymer-nanosphere compatibility, with up to 78% of 125I tetramine poloxamine 908 being displaced from poly(lactide-co-glycolide) (PLGA) nanospheres in 24 h, compared with 20% displacement of 125I tetramine poloxamine 908 in 24 h from polystyrene nanospheres. These results have direct implication for the future design of drug delivery systems based on coated nanospheres.     

奇异值方法在极化滤波中的应用

多波多分量技术不断发展,对波场分离的精度要求也日益提高,极化滤波是分离波场的有效手段。本文通过奇异值方法对原有特征值构建的极化滤波器进行了改进,得到了效果更好的极化滤波器。     

Improved localization in a generalized Hough scheme for the detection of straight edges

Hough transforms are widely used for the location of straight edges in digital images, yet most common line parametrization schemes give no information on longitudinal localization. The generalized Hough transform goes some way to overcoming this problem. This paper studies how to improve the situation further. A trade-off between sensitivity and localization is found; in practical situations this results in significantly greater accuracy, but the important gain is a reduction in the number of ambiguities introduced by interactions between the transforms of unrelated straight edges.     

自行火炮铅垂方向重心位置测量

目的　提出一种测量自行火炮铅垂方向重心位置的实验方法 .方法　使自行火炮沿射向倾斜一角度 ,用称重传感器测出火炮一端的重量 .结果　用一例题验证了文中方法和原理的正确性 .结论　所述方法正确可行     

基于Matlab的遗传算法解决多峰RBF神经网络模型全局最优化问题

以多峰RBF神经网络模型为例,研究了结合Matlab神经网络工具箱和遗传算法工具箱建立神经网络模型并求解模型的全局最优解,同时比较该模型中不同交叉算子对遗传算法全局寻优能力的影响.结果表明,采用启发式交叉算子能有效抑制遗传算法的早熟,提高其全局寻优能力,为用遗传算法解决多峰神经网络模型全局最优化问题提供了一种有效的途径.     

Random 3-SAT: The Plot Thickens

This paper presents an experimental investigation of the following questions: how does the average-case complexity of random 3-SAT, understood as a function of the order (number of variables) for fixed density (ratio of number of clauses to order) instances, depend on the density? Is there a phase transition in which the complexity shifts from polynomial to exponential in the order? Is the transition dependent or independent of the solver? Our experiment design uses three complete SAT solvers embodying different algorithms: GRASP, CPLEX, and CUDD. We observe new phase transitions for all three solvers, where the median running time shifts from polynomial in the order to exponential. The location of the phase transition appears to be solver-dependent. GRASP shifts from polynomial to exponential complexity near the density of 3.8, CPLEX shifts near density 3, while CUDD exhibits this transition between densities of 0.1 and 0.5. This experimental result underscores the dependence between the solver and the complexity phase transition, and challenges the widely held belief that random 3-SAT exhibits a phase transition in computational complexity very close to the crossover point.     

齿轮传动的CAD软件

在机械传动装置中,广泛地应用着齿轮传动。外啮合圆柱齿轮、内啮合圆柱齿轮、圆维齿轮和蜗轮蜗杆传动是齿轮传动的基本形式。我们在长期工作研究的基础上,建立了一套CAD软件。该软件可自动进行以上四种传动形式的优化设计,可通过人机交互完成齿轮的结构设计,用有限元分析计算齿轮的强度,并可在绘图机上绘制齿轮的工作图。该软件适用性强、使用方便,经过简单的修改便可用于其它机械产品的CAD。     

高精度机械伺服系统的一种新型自适应滑模控制方法

针对高精度机械伺服系统,提出一种高性能的新型自适应滑模控制方法,使闭环系统渐近跟踪给定的参考模型.该方法对转动惯量的大范围变化及非线性摩擦等外干扰均具有很强的鲁棒性.该方法的主要思想是用滑模方法抑制系统中的外部力矩扰动,对系统参数进行自适应估计,用估计值来补偿转动惯量的变化.对于控制算法的全局稳定性,采用李雅普诺夫直接法给出了严格的证明.该算法简单,其实现不需要误差的高阶微分信号,适于实时控制.本文方法以某高精度飞行仿真转台为例,对提出方法进行了实验研究,结果表明了该方法具有良好的跟踪性能,暂态响应和鲁棒性.     

雷达寻的制导半实物仿真误差研究

在介绍雷达寻的制导控制基本原理、半实物基本方法及仿真原理的基础上,详细介绍了雷达寻的制导控制半实物仿真系统的组成、各关键设备的工作原理并进行了主要误差因素分析,同时对关键仿真设备的误差进行研究,并建立数学模型,进一步推导出此类雷达仿真系统具有一般意义的仿真误差模型.最后通过型号半实物仿真试验对仿真系统的仿真精度进行验证.仿真试验表明,该半实物仿真系统的仿真精度满足控制系统半实物仿真试验的要求.     

一种快速切除HVDC输电系统接地极线路接地故障的方案

兴安直流输电系统逆变侧接地极线路与直流线路同杆并架敷设.在直流线路遭雷击后,接地极线路因感应过电压继发接地故障,在高压直流(HVDC)输电系统单极故障重启不成功的条件下,两回并行的接地极线路不平衡电流保护动作,导致直流输电系统双极闭锁.为了彻底消除接地极线路故障导致的HVDC输电系统闭锁现象,提出在换流站接地极线路出口处装设直流断路器灭弧的方案,并相应调整了接地极线路的控制和保护策略.