Comparative analysis of the virion structural proteins of 3 nuclear polyhedrosis viruses

The composition of structural proteins of virions of nuclear polyhedrosis viruses (NPV) of Barathra brassicae L., Lymantria dispor L. and Aporia crataegi L. was studied by polyacrylamide gel-SDS electrophoresis (PAGE-SDS). The object of the study included fractions of virion bundles in the envelope. All the baculoviruses under study were shown to have in virion structural proteins 23-24 polypeptides with molecular weights ranging from 12500 to 95000 daltons. Alongside with polypeptides of similar molecular weight, each baculovirus was shown to have a specific set of structural proteins allowing them to be readily identified. Specific distribution of major polypeptides of the virions was found. All the protein fractions were readily reproducible in repeated analyses with the exception of two minor polypeptides of NPV of Barathra brassicae L. The study showed that the composition of structural proteins of virions may be an important criterion for identification of baculoviruses under identical electrophoresis conditions.     

Structural and immunologic studies of Proteus mirabilis 033 O-specific polysaccharide

A variety of adrenal imaging agents have been used in nuclear medicine, but no agent has been developed for magnetic resonance (MR) imaging. The authors have previously observed accumulation of aminated macromolecules in adrenal glands. They now report the synthesis of a model polymeric aminated contrast agent for enhanced MR imaging of the adrenal glands. The model agent consisted of a poly-L-lysine conjugate (molecular weight, 245 kd) that had 70% free epsilon amino groups and 30% diethylenetriaminepentaacetic acid (DTPA)-derivatized amino groups to bind indium-111 or gadolinium. One hour after intravenous administration of this compound, adrenal uptake was 10.1% +/- 0.7 of injected dose per gram of tissue. When all free epsilon amino groups of the polylysine were completely substituted with DTPA, adrenal uptake was 3.4 times lower, indicating the importance of free amino groups for adrenal uptake. MR imaging in rats showed that a dose of 0.08 mmol of gadolinium per kilogram of the agent was sufficient to enhance the signal intensity of adrenal glands. There hours after intravenous administration of the agent, signal intensity of the adrenal glands was 186% of precontrast values (liver, 165%; kidney, 91%). Fluorescence microscopy showed that the agent accumulated primarily in the cortical zona glomerulosa and in the adrenal medulla. These initial studies demonstrate the feasibility of designing contrast agents for MR imaging of the adrenal glands.     

Numerical Modeling of Georadar Signal Reflection from a Conducting Object

Based on the presented results of numerical modeling of electromagnetic signal sent by a georadar and reflected by an underground object, it is shown that electromagnetic properties of the ground and the frequency of a transmitter influence the reflected signal parameters. The offered mathematical modeling procedure is of use to interpreting experimental data as it allows simulating reflection of any georadar signal in a media with any dielectric parameters.     

EFFECT OF NATIVE AND LYOPHILIZED KEFIR GRAINS ON SENSORY AND PHYSICAL ATTRIBUTES OF WHEAT BREAD

Native and lyophilized kefir grains were added directly to bread dough or as a starter in sourdough. Because of inadequate leavening activity of kefir, yeast‐leavened breads were prepared. Kefir addition influenced the quality attributes and the shelf life of wheat bread. The pH of bread containing kefir decreased as compared to that in the control bread. The acidity of bread with kefir increased as compared to that in the control bread. An addition of kefir grains, regardless of the form, lowered the bread volume and increased the mold‐free shelf life from 4 (control) to 5–7 days. The addition of kefir grains in bread dough contributed to a milder taste, a more delicate yogurt‐like or dairy aroma. The breads made with sourdoughs containing native or lyophilized kefir grains scored higher for crumb quality number than breads made by directly adding kefir grains.     

Application of nutritional formulare in the dietotherapy in patients with cardiovascular diseases

It was found that the including of nutritional formulae in the dietary therapy for patients with cardiovascular diseases and obesity has been evaluated, had a positive effect on the clinical presentation of disease, lipid metabolism and the dynamic of body composition of patients.     

Hematopoiesis in dogs irradiated with protons in lethal doses with screening of the bone marrow]

The paper presents the results of a 6-year investigation of hemopoiesis of dogs which were exposed to double irradiations (with a 1.5-month interval) with protons at a dose of 350 rad when different parts of the body (head, chest, abdomen, pelvis) were shielded. Throughout the entire period the dogs showed moderate leucopenia and by the end of the investigation insignificant anemia. The dogs irradiated with shielded abdomen displayed disturbances in bone marrow hemopoiesis, whereas other dogs exhibited relative, although transient, normalization. All the dogs showed a significant decrease of erythropoiesis and a relative stability of leucopoiesis as well as a significant elevation of the number of reticulocytes, monocytes and plasmocytes 5.5 year after irradiation.     

Nutrition and blood lipid profile in adolescents during socioeconomic reforms in Russia: epidemiological study

Aim of study was to assess trends in lipid profile and nutrition of adolescent population in Novosibirsk during the social and economic reforms in Russia (1989-1999). Three cross-sectional studies of school aged 14-17 in 1989 (657), in 1994 (620) and in 1999 (626) were carried out. Total sample was 1903 (914 males and 989 females). Blood lipids, such as total cholesterol (TC0, high-density lipoprotein cholesterol (HDL-C), and triglycerides (TG), were measured. Diet was estimated with the method of 24-hour dietary recall. During 10-year period mean serum TC decreased from 175 to 158 mg/dl in males and from 191 to 175 mg/dl in females (p < 0.001 for both genders). Females had significantly higher mean NC than did males at all surveys (p < 0.001)/Between 1989 and 1994 mean HDL-C levels decreased in boys both (p < 0.001), but in 1999 these levels were similar to 1989. During 1989-1994, TG levels significantly decreased, but between 1994 and 1999 mean TG levels increased in 1.5 times in both gender groups. Prevalence of high TC (> = 2000 mg/dl) significantly decreased from 17% to 6% in males and from 28% to 14% in females. Frequencies of low HDL-C (> = 40 mg/dl) also decreased in boys (from 28 to 8%) and girls (from 13 to 3%). Trends in diet showed similar changes and characterized by significant decreasing of total energy intakes (from 3021 to 2342 kcal in boys and from 2300 to 1644 kcal in girls) and of basic nutrient intakes (proteins? Fats? Carbohydrates). Negative trends in blood lipid profile and diet in adolescents during the unstable period of socioeconomic reforms in Russia indicate on serious changes in metabolic processes and need in large state economic programs to improve the situation.