The heterogeneity of cell populations by the cytochemical characteristics of the cellular chromatin

Cell heterogeneity of cytochemical characteristics of chromatin in the norm and after provocative factors in vitro (a heating at 45 degrees C, X-irradiation and joint actions of heating and X-ray irradiation) was investigated in the peripheral blood mononuclear cells of sheep and mouse, the lymphoid cell populations of the murine bone marrow, spleen and thymus. As a criterion of cytochemical heterogeneity were used the distinctions in the rate of staining of cell nuclei with DNA-specific dyes, that was registered by means of measurement of coefficient variation of the DNA-histogram basis peak, with a flow cytometry. Heterogeneity of cytochemical characteristics are dealt with showed a variability of spatial organization of interphase nuclei of single cells. The provocative factors bring about a decrease in the level of the cytochemical characteristics heterogeneity in the sheep mononuclear cells. In the murine cell populations studied no such equalizing of the marker of variability was revealed.     

A pulse-type evaporator for ultrafast deposition of thin films in ultrahigh vacuum

The design of a simple pulse-type evaporator ensuring a high rate (up to ～10⁴ nm/s) of deposition of thin films (0.01–100 nm thick) onto a solid surface in ultrahigh vacuum is described. The evaporation pulse is created by discharge of a capacitor bank via a fast-response evaporator produced from a tungsten foil. Results illustrating the efficiency of this device are presented.     

Yield of products from catalytic cracking of vacuum gasoils

In catalytic cracking of vacuum gasoils, the yields of products are correlated with their hydrogen content. A correlation that allows predicting the yields of products of this process was elaborated based on the results of cracking of 11 gasoils of different origin and data on their density, refractive index, and 50% distillation temperature. However, it is not possible to predict the octane number of the naphtha obtained in cracking with the group hydrocarbon composition of the feedstock. __________ Translated from Khimiya i Tekhnologiya Topliv i Masel, No. 4, pp. 31–34, July–August, 2007.     

Identification of an interaction between the m-band protein skelemin and beta-integrin subunits. Colocalization of a skelemin-like protein with beta1- and beta3-integrins in non-muscle cells

Signaling across integrins is regulated by interaction of these receptors with cytoskeletal proteins and signaling molecules. To identify molecules interacting with the cytoplasmic domain of the beta3-integrin subunit (glycoprotein IIIa), a placental cDNA library was screened in the yeast two-hybrid system. Two identical clones coding for a 96-amino acid sequence were identified. This sequence was 100% identical to a sequence in skelemin, a protein identified previously in skeletal muscle. Skelemin is a member of a superfamily of cytoskeletal proteins that contain fibronectin-type III-like motifs and immunoglobulin C2-like motifs and that regulate the organization of myosin filaments in muscle. The amino acid residues in the isolated clones encompassed C2 motifs 4 and 5 of skelemin. A recombinant skelemin protein consisting of C2 motifs 3-7 interacted with beta1- and beta3-integrin cytoplasmic domains expressed as glutathione S-transferase (GST) fusion proteins, but not with GST-beta2-integrin cytoplasmic tail or GST alone. The skelemin-binding region was in the membrane proximal cytoplasmic domains of the integrins. Full-length skelemin interacted with integrin in intact cells as demonstrated by the colocalization of hemagglutinin-tagged skelemin in Chinese hamster ovary (CHO) cells containing alphaIIbbeta3-integrin and by the finding that microinjection of C2 motif 4 of skelemin into C2C12 mouse myoblast cells caused spread cells to round up. A skelemin-like protein was detected in CHO cells, endothelial cells, and platelets, and this protein colocalized with beta1- and beta3-integrins in CHO cells. This study suggests the presence of a skelemin-like protein in non-muscle cells and provides evidence that it may be involved in linking integrins to the cytoskeleton.     

Breast abscess with lethal septicemia due to Pseudomonas aeruginosa in a patient with AIDS

OBJECTIVE: The incidence of Pseudomonas aeruginosa in HIV-infected patients has increased over the last years. We describe a case of pseudomonal breast abscess complicated with fatal septicemia in an AIDS patient. CASE REPORT: A 21-year-old woman was admitted for fever, chills, nausea, vomiting and pain in the breast. She had a swelling in the right breast of 3 days duration. HIV infection had been confirmed 6 years earlier. CD4 count was 2/mm3. Surgical drainage produced a blue-green purulent discharge which grew Pseudomonas aeruginosa on culture. Despite cloxacilin, then ceftazidime and amikacin, initial improvement was followed 2 weeks later by nodular pulmonary infiltration with cavitation. P. aeruginosa was recovered from sputum and blood cultures, but stepwise resistance developed and the patient died 3 months after admission. DISCUSSION: Breast abscesses are infrequent in nonlactating women. P. aeruginosa is rarely involved, even in HIV patients. Due to the risk of resistance, prompt administration of appropriate antibiotics is required.     

The effect of a two-week anti-orthostatic hypokinesia on the cardiac function and on the latter''s regulation in monkeys

Medical practice guidelines are being developed rapidly and advocated as a crucial component in transforming and improving the delivery of health care services. However, little is known about the ability of hospitals to institute guidelines outside of experimental settings. This article approaches guideline implementation from the viewpoint of process innovation assimilation and presents specific recommendations for organizational tactics leading to successful implementation of practice guidelines.     

Gastroparesis after celiac plexus block

Pectin constituents, which were about 70 w/w% of extracellular polysaccharides (ECP) from a cell-suspension culture of Mentha, were purified by gel filtration chromatography, and their sugar composition and linkage were investigated. Two major constituents identified were (1-->3)-linked galactan carrying arabinosyl residues on C-6 and (1-->4)-alpha-linked galacturonan partially interspersed with (1-->2)-linked rhamnosyl resides. Acetylated or methylated pectins were not identified on 1H-NMR analysis.     

Production of high-alumina cement clinker by fusion in an electric furnace

Conclusions The possibility of obtaining clinker from high-alumina cement by melting a mixture of lime and technical alumina in an electric-arc furnace at the Klyuchevsk Ferroalloy Plant has been shown.The high-alumina cement obtained from fused clinker is significantly better in its physicochemical properties than the cement produced on the basis of the lime-alumina slags from the ferroalloy industry.Translated from Ogneupory, No. 5, pp. 25–27, May, 1984.     

Rheological properties of spinning compositions for spinning biocatalyst fibre

Conclusions Rheological properties of protein-containing spinning compositions intended for spinning biocatalyst fibres have been studied.The rheological properties of these spinning compositions depend on the composition of the aqueous phase, the concentration of protein, and the modification of the protein; this is explained by a change in state of the protein at the interface and in the bulk of the aqueous phase.Modification of the protein leads to an increase in stability of the process of spinning the biocatalyst fibre by the wet method.Moscow Textile Institute. Translated from Khimicheskie Volokna, No. 1, pp. 9–11, January–February, 1987.