In vitro inhibition of Escherichia coli O157:H7 by bifidobacterial strains of human origin

The ability of bifidobacteria isolated from infant feces to inhibit enterohemorrhagic Escherichia coli serotype O157:H7 in vitro and reduce its adhesion to human enterocyte-like Caco-2 cells was evaluated in comparison to American Type Culture Collection bifidobacterial reference strains. Five Bifidobacterium isolates from infant feces were identified and characterized by morphology, fructose-6-phosphate phosphoketolase (F6PPK) assay, polymerase chain reaction using bifidobacterial 16S rDNA specific primers, carbohydrate fermentation patterns, resistance to lysozyme, acid, bile and hydrogen peroxide as well as their ability to inhibit E. coli O157:H7 using the agar spot technique. Infant isolates showed greater resistance to bile, acid, lysozyme and more antimicrobial activity against E. coli O157:H7 than ATCC strains. Two infant isolates identified as B. bifidum RBL 71 and B. bifidum RBL 460 showed good adhesion and significant potential for reducing adhesion of E. coli O157:H7 to Caco-2 cells. This effect was dependent on bifidobacterial cell concentration. These results show that bifidobacteria isolated from infants may be useful for improving probiotic formulae with respect to protection against E. coli O157:H7 infection.     

Eccentrically loaded concrete encased steel composite columns

This paper presents a nonlinear 3-D finite element model for eccentrically loaded concrete encased steel composite columns. The columns were pin-ended subjected to an eccentric load acting along the major axis, with eccentricity varied from 0.125 to 0.375 of the overall depth (D) of the column sections. The model accounted for the inelastic behaviour of steel, concrete, longitudinal and transverse reinforcement bars as well as the effect of concrete confinement of the concrete encased steel composite columns. The interface between the steel section and concrete, the longitudinal and transverse reinforcement bars, and the reinforcement bars and concrete were also considered allowing the bond behaviour to be modelled and the different components to retain its profile during the deformation of the column. The initial overall geometric imperfection was carefully incorporated in the model. The finite element model has been validated against existing test results. The concrete strengths varied from normal to high strength (30–110 MPa). The steel section yield stresses also varied from normal to high strength (275–690 MPa). Furthermore, the variables that influence the eccentrically loaded composite column behaviour and strength comprising different eccentricities, different column dimensions, different structural steel sizes, different concrete strengths, and different structural steel yield stresses were investigated in a parametric study. Generally, it is shown that the effect on the composite column strength owing to the increase in structural steel yield stress is significant for eccentrically loaded columns with small eccentricity of 0.125D. On the other hand, for columns with higher eccentricity 0.375D, the effect on the composite column strength due to the increase in structural steel yield stress is significant for columns with concrete strengths lower than 70 MPa. The strength of composite columns obtained from the finite element analysis were compared with the design strengths calculated using the Eurocode 4 for composite columns. Generally, it is shown that the EC4 accurately predicted the eccentrically loaded composite columns, while overestimated the moment.     

Effect of Transverse Reinforcement on the Flexural Behavior of Continuous Concrete Beams Reinforced with FRP

Continuous concrete beams are structural elements commonly used in structures that might be exposed to extreme weather conditions and the application of deicing salts, such as bridge overpasses and parking garages. In such structures, reinforcing continuous concrete beams with the noncorrodible fiber-reinforced polymer (FRP) bars is beneficial to avoid steel corrosion. However, the linear-elastic behavior of FRP materials makes the ability of continuous beams to redistribute loads and moments questionable. A total of seven full-scale continuous concrete beams were tested to failure. Six beams were reinforced with glass fiber-reinforced polymer (GFRP) longitudinal bars, whereas one was reinforced with steel as control. The specimens have rectangular cross section of 200×300??mm and are continuous over two spans of 2,800?mm each. Both steel and GFRP stirrups were used as transverse reinforcement. The material, spacing, and amount of transverse reinforcement were the primary investigated parameters in this study. In addition, the experimental results were compared with the code equations to calculate the ultimate capacity. The experimental results showed that moment redistribution in FRP-reinforced continuous concrete beams is possible and is improved by increasing the amount of transverse reinforcement. Also, beams reinforced with GFRP stirrups illustrated similar performance compared with their steel-reinforced counterparts.     

Durability of GFRP Bars’ Bond to Concrete under Different Loading and Environmental Conditions

In the last decade, noncorrodible fiber-reinforced polymer (FRP) reinforcing bars have been increasingly used as the main reinforcement for concrete structures in harsh environments. Also, owing to their lower cost compared with other types of FRP bars, glass-FRP (GFRP) bars are more attractive to the construction industry, especially for implementation in bridge deck slabs. In North America, bridge deck slabs are exposed to severe environmental conditions, such as freeze-thaw action, in addition to traffic fatigue loads. Although the bond strength of GFRP bars has been proved to be satisfactory, their durability performance under the dual effects of fatigue-type loading and freeze-thaw action is still not well understood. Few experimental test data are available on the bond characteristics of FRP bars in concrete elements under different loading and environmental conditions. This research investigates the individual and combined effects of freeze-thaw cycles along with sustained axial load and fatigue loading on the bond characteristics of GFRP bars embedded in concrete. An FRP-reinforced concrete specimen was developed to apply axial-tension fatigue or sustained loads to GFRP bars within a concrete environment. A total of thirty-six test specimens was constructed and tested. The test parameters included bar diameter, concrete cover thickness, loading scheme, and environmental conditioning. After conditioning, each specimen was sectioned into two halves for pullout testing. Test results showed that fatigue load cycles resulted in approximately 50% loss in the bond strength of sand-coated GFRP bars to concrete, while freeze-thaw cycles enhanced their bond to concrete by approximately 40%. Larger concrete covers were found more important in cases of larger bar sizes simultaneously subjected to fatigue load and freeze-thaw cycles.     

Construction and Testing of an Innovative Concrete Bridge Deck Totally Reinforced with Glass FRP Bars: Val-Alain Bridge on Highway 20 East

The Val-Alain Bridge, located in the Municipality of Val-Alain on Highway 20 East, crosses over Henri River in Québec, Canada. The bridge is a slab-on-girder type with a skew angle of 20° over a single span of 49.89?m and a total width of 12.57?m. The bridge has four simply supported steel girders spaced at 3,145?mm. The deck slab is a 225-mm-thick concrete slab, with semi-integral abutments, continuous over the steel girders with an overhang of 1,570?mm on each side. The concrete deck slab and the bridge barriers were reinforced with glass fiber reinforced polymer (GFRP) reinforcing bars utilizing high-performance concrete. The Val-Alain Bridge is the Canada’s first concrete bridge deck totally reinforced with GFRP reinforcing bars. Using such nonmetallic reinforcement in combination with high-performance concrete leads to an expected service life of more than 75?years. The bridge is well instrumented with electrical resistance strain gauges and fiber-optic sensors at critical locations to record internal strain data. Also, the bridge was tested for service performance using calibrated truckloads. Design concepts, construction details, and results of the first series of live load field tests are presented.     

Designing and Testing of Concrete Bridge Decks Reinforced with Glass FRP Bars

In addition to their high strength and light weight, fiber-reinforced polymer (FRP) composite reinforcing bars offer corrosion resistance, making them a promising alternative to traditional steel reinforcing bars in concrete bridge decks. FRP reinforcement has been used in several bridge decks recently constructed in North America. The Morristown Bridge, which is located in Vermont, United States, is a single span steel girder bridge with integral abutments spanning 43.90 m. The deck is a 230 mm thick concrete continuous slab over girders spaced at 2.36 m. The entire concrete deck slab was reinforced with glass FRP (GFRP) bars in two identical layers at the top and the bottom. The bridge is well instrumented at critical locations for internal temperature and strain data collection with fiber-optic sensors. The bridge was tested for service performance using standard truck loads. The construction procedure and field test results under actual service conditions revealed that GFRP rebar provides very good and promising performance.     

Field Investigation on the First Bridge Deck Slab Reinforced with Glass FRP Bars Constructed in Canada

Recently, there has been a rapid increase in using noncorrosive fiber-reinforced polymers (FRP) reinforcing bars as alternative reinforcement for bridge deck slabs, especially those in harsh environments. A new two-span girder type bridge, Cookshire-Eaton Bridge (located in the municipality of Cookshire, Quebec, Canada), was constructed with a total length of 52.08 m over two equal spans. The deck was a 200-mm-thick concrete slab continuous over four spans of 2.70 m between girders with an overhang of 1.40 m on each side. One full span of the bridge was totally reinforced using glass fiber-reinforced polymer (GFRP) bars, while the other span was reinforced with galvanized steel bars. The bridge deck was well instrumented at critical locations for internal temperature and strain data collection using fiber optic sensors. The bridge was tested for service performance using calibrated truckloads as specified by the Canadian Highway Bridge Design Code. The construction procedure and field test results under actual service conditions revealed that GFRP rebar provides very competitive performance in comparison to steel.     

Would LEED-UHI greenery and high albedo strategies mitigate climate change at neighborhood scale in Cairo,Egypt?

Neighborhood has always been of significant interest to built environment stockholders as a basic planning unit. However, any discussion in these concerns, without drawing attention to sustainable microclimate approaches, would still in a mess at a time of increasing population and climate change. Emergence of the sustainable development concept at the mid-20th century and its emphasis led to increasing crucial role that the urban green infrastructure along with reflective materials can play in mitigating neighborhood microclimate’s symptoms of climate change. Considering the lack of studies for urban heat island (UHI) in hot arid regions, particularly in Egypt and the limited number of studies concerning the numerical simulation of all mitigation strategies incorporated, this research studies the mitigation of UHI phenomenon in a case study in Cairo in present and future (2020, 2050 and 2080) through applying the criteria of tree lines, green roofs, high albedo pavements and shading structures within the neighborhood sustainability assessment tool (Leadership in Energy and Environmental Design for Neighborhood; LEED-ND). The microclimatic numerical CFD simulations of ENVI-met 4.0 was used following the measurement of LAI and Albedo of selected Egyptian trees to assess UHI through air and radiant temperature differences before and after applying mitigation strategies. Results demonstrate a considerable ability to acclimatize the microclimate in terms of better conditions in present and future.