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21.
Marion Woytasik Johan Moulin Emile Martincic Anne-Lise Coutrot Elisabeth Dufour-Gergam 《Microsystem Technologies》2008,14(7):951-956
Recent advances in microtechnology allow realization of planar microcoils. These components are integrated in MEMS as magnetic sensor or actuator. In the latter case, it is necessary to maximize the effective magnetic field which is proportional to the current passing through the copper track and depends on the distance to the generation microcoil. The aim of this work was to determine the optimal microcoil design configuration for magnetic field generation. The results were applied to magnetic actuation, taking into account technological constraints. In particular, we have considered different realistic configurations that involve a magnetically actuated device coupled to a microcoil. Calculations by a semi-analytical method using Matlab software were validated by experimental measurements. The copper planar microcoils are fabricated by UV micromoulding on different substrates: flexible polymer (Kapton®) and silicate on silicon. They are constituted by a spiral-like continuous track. Their total surface is about 1 mm2. 相似文献
22.
Gian Luigi Angrisani Piriya Taptimthong Susanne Elisabeth Thürer Christian Klose Hans Jürgen Maier Marc Christopher Wurz Kai Möhwald 《Advanced Engineering Materials》2018,20(9)
23.
van Midwoud PM Merema MT Verpoorte E Groothuis GM 《Journal of laboratory automation》2011,16(6):468-476
Early information on the metabolism and toxicity properties of new drug candidates is crucial for selecting the right candidates for further development. Preclinical trials rely on cell-based in vitro tests and animal studies to characterize the in vivo behavior of drug candidates, although neither are ideal predictors of drug behavior in humans. Improving in vitro systems for preclinical studies both from a technological and biological model standpoint thus remains a major challenge. This article describes how microfluidics can be exploited to come closer to this goal in combination with precision-cut liver slices (PCLS) as an improved organomimetic system. Recently, we developed a novel microfluidic-based system incorporating a microchamber for slice perifusion to perform drug metabolism studies with mammalian PCLS under continuous flow. In the present study, the viability and metabolism of human PCLS were assessed by the measurement of the leakage of liver-specific enzymes and metabolism of four different substrates: lidocaine, 7-hydroxycoumarin, 7-ethoxycoumarin, and testosterone. All experiments were verified with well plates, an excellent benchmark for these experiments. Clearly, however, human tissue is not readily available, and it is worth considering how to perform a maximum number of informative experiments with small amounts of material. In one approach, the microfluidic system was coupled to an HPLC system to allow on-line monitoring and immediate detection of unstable metabolites, something that is generally not possible with conventional well-plate systems. This novel microfluidic system also enables the in vitro measurement of interorgan interactions by connecting microchambers containing different organ slices in series for sequential perfusion. This versatile experimental system has the potential to yield more information about the metabolic profiles of new drug candidates in human and animal tissues in an early stage of development compared with well plates alone. 相似文献
24.
The effect of the temporal resolution of NDVI data on season onset dates and trends across Canadian broadleaf forests 总被引:2,自引:0,他引:2
Angela Kross Richard Fernandes Elisabeth Beaubien 《Remote sensing of environment》2011,115(6):1564-1575
Satellite remote sensing has the potential to contribute to plant phenology monitoring at spatial and temporal scales relevant for regional and global scale studies. Historically, temporal composites of satellite data, ranging from 8 days to 16 days, have been used as a starting point for satellite-derived phenology data sets. In this study we assess how the temporal resolution of such composites affects the estimation of the start of season (SOS) by: 1) calibrating a relationship between satellite derived SOS with in situ leaf unfolding (LU) of trembling aspen (Populus tremuloides) across Canada and 2) quantifying the sensitivity of calibrated satellite SOS estimates and trends, over Canadian broadleaf forests, to the temporal resolution of NDVI data. SOS estimates and trends derived from daily NDVI data were compared to SOS estimates and trends derived from multiday NDVI composites that retain the exact date of the maximum NDVI value or that assume the midpoint of the multiday interval as the observation date. In situ observations of LU dates were acquired from the PlantWatch Canada network. A new Canadian database of cloud and snow screened daily 1-km resolution National Oceanic and Atmospheric Administration advanced very high resolution radiometer surface reflectance images was used as input satellite data. The mean absolute errors of SOS dates with respect to in situ LU dates ranged between 13 and 40 days. SOS estimates from NDVI composites that retain the exact date of the maximum NDVI value had smaller errors (~ 13 to 20 days). The sensitivity analysis reinforced these findings: SOS estimates from NDVI composites that use the exact date had smaller absolute deviations from the LU date (0 to − 5 days) than the SOS estimates from NDVI composites that use the midpoint (− 2 to − 27 days). The SOS trends between 1985 and 2007 were not sensitive to the temporal resolution or compositing methods. However, SOS trends at individual ecozones showed significant differences with the SOS trends from daily NDVI data (Taiga plains and the Pacific maritime zones). Overall, our results suggest that satellite based estimates of vegetation green-up dates should preferably use sub-sampled NDVI composites that include the exact observation date of the maximum NDVI to minimize errors in both, SOS estimates and SOS trend analyses. For trend analyses alone, any of the compositing methods could be used, preferably with composite intervals of less than 28 days. This is an important finding, as it suggests that existing long-term 10-day or 15-day NDVI composites could be used for SOS trend analyses over broadleaf forests in Canada or similar areas. Future studies will take advantage of the growing in situ phenology networks to improve the validation of satellite derived green-up dates. 相似文献
25.
Steptoe Andrew; Perkins-Porras Linda; Rink Elisabeth; Hilton Sean; Cappuccio Francesco P. 《Canadian Metallurgical Quarterly》2004,23(6):574
This study assessed psychological and social factors predicting 12-month changes in fruit and vegetable consumption achieved by 271 men and women from a low-income population randomized to brief behavioral and nutrition education counseling. Greater increases in fruit and vegetable intake were achieved in the behavioral than in the nutrition education condition (1.49 vs. 0.87 portions per day, p=.021). Increases were predicted by baseline social support for dietary change but not by baseline psychological measures. However, short-term (8-week) changes in dietary self-efficacy, encouragement, anticipated regret, perceived benefits, and knowledge of recommended intake predicted 12-month changes in fruit and vegetable consumption independently of gender, age, ethnicity, income, and baseline intake. These factors accounted for 51% of the superiority of behavioral counseling over nutrition education. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
26.
Lukas Martin Patrick Koczera Nadine Simons Elisabeth Zechendorf Janine Hoeger Gernot Marx Tobias Schuerholz 《International journal of molecular sciences》2016,17(3)
Sepsis is the most common cause of death in intensive care units and associated with widespread activation of host innate immunity responses. Ribonucleases (RNases) are important components of the innate immune system, however the role of RNases in sepsis has not been investigated. We evaluated serum levels of RNase 1, 3 and 7 in 20 surgical sepsis patients (Sepsis), nine surgical patients (Surgery) and 10 healthy controls (Healthy). RNase 1 and 3 were elevated in Sepsis compared to Surgery (2.2- and 3.1-fold, respectively; both p < 0.0001) or compared to Healthy (3.0- and 15.5-fold, respectively; both p < 0.0001). RNase 1 showed a high predictive value for the development of more than two organ failures (AUC 0.82, p = 0.01). Patients with renal dysfunction revealed higher RNase 1 levels than without renal dysfunction (p = 0.03). RNase 1 and 3 were higher in respiratory failure than without respiratory failure (p < 0.0001 and p = 0.02, respectively). RNase 7 was not detected in Healthy patients and only in two patients of Surgery, however RNase 7 was detected in 10 of 20 Sepsis patients. RNase 7 was higher in renal or metabolic failure than without failure (p = 0.04 and p = 0.02, respectively). In conclusion, RNase 1, 3 and 7 are secreted into serum under conditions with tissue injury, such as major surgery or sepsis. Thus, RNases might serve as laboratory parameters to diagnose and monitor organ failure in sepsis. 相似文献
27.
28.
Elisabeth A. A. O'Flaherty Paraskevi Tsermoula Eileen E. O'Neill Nora M. O'Brien 《International Journal of Food Science & Technology》2019,54(4):1256-1264
Beef processing produces high volumes of protein rich, low value, ‘waste’ co-products such as offal. Beef improves uptake of low bioavailable non-haem iron (found in vegetables, fortificants, supplements) and this effect is dubbed the ‘meat-factor’, although the underlying mechanism is not fully understood. Here, we investigate whether bovine co-products (kidney, lung, heart) not previously studied share this enhancing potential. This was determined by coupled in vitro digestion of co-products and subsequent caco-2 cell ferritin formation (an intracellular iron storage protein). In this study we show that bovine co-products significantly increase caco-2 cells’ response to non-haem iron from infant rice cereal. The presence of these co-products, (kidney, lung and heart), increased relative uptake (by 207.13%, 171.21%, 265.28%, respectively), to a greater extent than beef (30.23%). Our findings present a novel function for co-products of beef processing that may have potential as food ingredients to improve non-haem iron bioavailability, thus adding value. 相似文献
29.