Justine Dumay  Marion Allery  Claire Donnay‐Moreno  Gilles Barnathan  Pascal Jaouen  Marie Elise Carbonneau  Jean Pascal Bergé 《Journal of the science of food and agriculture》2009,89(9):1599-1606

BACKGROUND: Fish by‐products are not considered as valuable raw materials even if they usually contain valuable components such as lipids. Fish lipids are well known for their nutritional potential and health effects but their extraction remains problematic due to the use of organic solvents. Enzymatic hydrolysis such as the proteolysis of tissues can lead to lipid extraction. RESULTS: Hydrolysis of sardine heads by Protamex was studied (temperature, hydrolysis time and enzyme–substrate ratio) using response surface methodology in order to obtain the highest release of lipids and particularly phospholipids. No relation between the degree of hydrolysis and lipid recovery were depicted; however, optimum conditions for both the release of lipids and phospholipids were found to be similar (29 min, 31 °C with 2.6 g kg^?1 enzyme). Under these hydrolysis conditions, rich lipid and phospholipid fractions (oily and aqueous fractions) can be recovered when time, temperature and enzyme consumption are minimized. Analytical data have revealed that they contain high‐quality lipids, especially ω3 fatty acid. CONCLUSION: This study demonstrated that proteolysis can be used for high lipid recovery from little‐exploited biomass like fish heads without requiring solvent or thermal treatment. Resulting phospholipids, fatty acids and peptides could be utilized for nutritional or feed purposes. Copyright © 2009 Society of Chemical Industry  相似文献   

    

Phung VT  Sælid E  Egelandsdal B  Volden J  Slinde E 《Journal of food science》2011,76(6):C929-C936

Abstract: The oxygen consumption rate (OCR) of 2 types of permeabilized tissues and their corresponding isolated mitochondria from porcine M. masseter and liver, resulting in 4 systems, was studied at different pH values (5.0 to 7.1) using fresh samples and samples frozen directly in liquid nitrogen (N₂) or air‐frozen at ?20°C. A protocol with the additive sequence rotenone–succinate–ADP (adenosine diphosphate)–cytochrome c–FCCP (carbonyl cyanide p‐trifluoromethoxyphenylhydrazone) was used to study respiration changes. The OCR of liver respiring on succinate (OCR _S ) was higher than that of muscle tissue. pH had a larger effect on OCR_S than freeze‐thawing. Low pH was associated with reduced OCR_S. The OCR_S of isolated muscle mitochondria appeared to be an underestimated relative to the OCR_S of permeabilized muscle cells. Increasing pH, following prior subjection to pH 5.0, showed partial reversibility of the OCR_S. The freeze‐thaw cycle increased the OCR_S when muscle systems were frozen and examined above pH 6.0; this effect was less apparent for liver tissue. A response to cytochrome c addition, indicating a defective outer mitochondrial membrane, was observed for all 4 systems. The response was, however, lowest for permeabilized cells. The ADP/FCCP additive pair indicated partial coupling for isolated liver and muscle mitochondria. These additives gave weak responses for the permeabilized liver cells while the OCR seemed to be inhibited for permeabilized muscle fibers when ADP/FCCP was added. Practical Application: The mitochondrial state is believed to be important for myoglobin reduction, development of flavor, and possibly other meat qualities. By monitoring the oxygen consumption in mitochondria and meat we can better understand and control such processes following freezing and thawing.  相似文献   

    

Damia Mawad  Elise Stewart  David L. Officer  Tony Romeo  Pawel Wagner  Klaudia Wagner  Gordon G. Wallace 《Advanced functional materials》2012,22(13):2692-2699

Conducting polymers (CPs) have exciting potential as scaffolds for tissue engineering, typically applied in regenerative medicine applications. In particular, the electrical properties of CPs has been shown to enhance nerve and muscle cell growth and regeneration. Hydrogels are particularly suitable candidates as scaffolds for tissue engineering because of their hydrated nature, their biocompatibility, and their tissue‐like mechanical properties. This study reports the development of the first single component CP hydrogel that is shown to combine both electro‐properties and hydrogel characteristics. Poly(3‐thiopheneacetic acid) hydrogels were fabricated by covalently crosslinking the polymer with 1,1′‐carbonyldiimidazole (CDI). Their swelling behavior was assessed and shown to display remarkable swelling capabilities (swelling ratios up to 850%). The mechanical properties of the networks were characterized as a function of the crosslinking density and were found to be comparable to those of muscle tissue. Hydrogels were found to be electroactive and conductive at physiological pH. Fibroblast and myoblast cells cultured on the hydrogel substrates were shown to adhere and proliferate. This is the first time that the potential of a single component CP hydrogel has been demonstrated for cell growth, opening the way for the development of new tissue engineering scaffolds.  相似文献   

    

Hans-Otto Bhm  Mazyar Yazdani  Elise Mrk Sands  Anja steby Vassli  Erle Kristensen  Helge Rootwelt  Hanne Bendiksen Skogvold  Eylert Brodtkorb  Katja Benedikte Prest Elgsten 《International journal of molecular sciences》2022,23(24)

Pyridoxine-dependent epilepsy (PDE) is a rare autosomal recessive developmental and epileptic encephalopathy caused by pathogenic variants in the ALDH7A1 gene (PDE-ALDH7A1), which mainly has its onset in neonates and infants. Early diagnosis and treatment are crucial to prevent severe neurological sequelae or death. Sensitive, specific, and stable biomarkers for diagnostic evaluations and follow-up examinations are essential to optimize outcomes. However, most of the known biomarkers for PDE lack these criteria. Additionally, there is little discussion regarding the interdependence of biomarkers in the PDE-ALDH7A1 metabolite profile. Therefore, the aim of this study was to understand the underlying mechanisms in PDE-ALDH7A1 and to discover new biomarkers in the plasma of patients using global metabolomics. Plasma samples from 9 patients with genetically confirmed PDE-ALDH7A1 and 22 carefully selected control individuals were analyzed by ultra high performance liquid chromatography–high-resolution mass spectrometry (UHPLC-HRMS). Two novel and reliable pyridoxine-independent diagnostic markers, 6-hydroxy-2-aminocaproic acid (HACA) and an isomer of C₉H₁₁NO₄, were identified. Furthermore, a possible reaction mechanism is proposed for HACA. This study demonstrates the capability of global metabolomics in disease screening to detect established and novel biomarkers.  相似文献   

    

Dwarakanath Ravikumar  Thomas Seager  Parikhit Sinha  Matthew P. Fraser  Serrae Reed  Elise Harmon  Alexis Power 《Progress in Photovoltaics: Research and Applications》2020,28(9):887-898

Photovoltaic waste is projected to reach up to 78 million tons across globally dispersed locations through 2050. Current recycling infrastructure is inadequate to process these waste volumes responsibly. This necessitates commercializing novel, environmentally advantageous photovoltaic recycling technologies that improve upon incumbent industrial operations. CdTe photovoltaic recycling is a promising candidate for improvements as 25,000 tons of spent modules are recycled worldwide annually. This paper evaluates the operational performance and compares six novel technologies, the incumbent technology used in industry and one technology which extends the incumbent process across ten environmental impact categories. The tradeoff between incurring a transportation burden (ship or road) to recycle in a large‐scale centralized facility with a higher operational efficiency and avoiding transportation by recycling in a small‐scale decentralized facility with a lower operational efficiency is evaluated. Thermal delamination to eliminate the ethylene vinyl acetate and separate the photovoltaic glass panels is preferable to the incumbent mechanical process across nine environmental impact categories and decreases the climate‐change impact of CdTe photovoltaic recycling by 23%. Bath and probe sonication are ineffective for delamination, and the use of organic solvents is more environmentally burdensome than the incumbent mechanical process. Centralized recycling with shipping is environmentally preferable than with road‐based transportation. For every 100‐km increase in road transportation from the decentralized to the centralized facility, the inventory requirement in the centralized facility should be 6% lower than the decentralized facility for centralized recycling to have a lower climate‐change impact than decentralized recycling. The corresponding value for shipping is 0.4%.  相似文献   

    

Diane Frankel  Isabelle Nanni  L&#x;Houcine Ouafik  Clara Camilla  Eric Pellegrino  Nathalie Beaufils  Laurent Greillier  Herv Dutau  Philippe Astoul  Elise Kaspi  Patrice Roll 《International journal of molecular sciences》2022,23(18)

The detection of ROS1 and ALK rearrangements is performed for advanced-stage non-small cell lung cancer. Several techniques can be used on cytological samples, such as immunocytochemistry (ICC), fluorescence in situ hybridization (FISH) and, more recently, next-generation sequencing (NGS), which is gradually becoming the gold standard. We performed a retrospective study to compare ALK and ROS1 rearrangement results from immunocytochemistry, FISH and NGS methods from 131 cytological samples. Compared to NGS, the sensitivity and specificity of ICC were 0.79 and 0.91, respectively, for ALK, and 1 and 0.87 for ROS1. Regarding FISH, the sensitivity and specificity were both at 1 for ALK and ROS1 probes. False-positive cases obtained by ICC were systematically corrected by FISH. When using ICC and FISH techniques, results are very close to NGS. The false-positive cases obtained by ICC are corrected by FISH, and the true-positive cases are confirmed. NGS has the potential to improve the detection of ALK and ROS1 rearrangements in cytological samples; however, the cost of this technique is still much higher than the sequential use of ICC and FISH.  相似文献   

    

Christina Zeitz  Ccile Mjcase  Christelle Michiels  Christel Condroyer  Juliette Wohlschlegel  Marine Foussard  Aline Antonio  Vanessa Dmontant  Lisa Emmenegger  Audrey Schalk  Marion Neuill  Elise Orhan  Sbastien Augustin  Crystel Bonnet  Amrit Estivalet  Frdric Blond  Steven Blanchard  Camille Andrieu  Sandra Chantot-Bastaraud  Thierry Lveillard  Saddek Mohand-Saïd  Jos-Alain Sahel  Isabelle Audo 《International journal of molecular sciences》2021,22(15)

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Elise Aaseb  Annette K. Brenner  Maria Hernandez-Valladares  Even Birkeland  Frode S. Berven  Frode Selheim   ystein Bruserud 《International journal of molecular sciences》2021,22(11)

Mesenchymal stem cells (MSCs) can differentiate into osteoblasts, and therapeutic targeting of these cells is considered both for malignant and non-malignant diseases. We analyzed global proteomic profiles for osteoblasts derived from ten and MSCs from six healthy individuals, and we quantified 5465 proteins for the osteoblasts and 5420 proteins for the MSCs. There was a large overlap in the profiles for the two cell types; 156 proteins were quantified only in osteoblasts and 111 proteins only for the MSCs. The osteoblast-specific proteins included several extracellular matrix proteins and a network including 27 proteins that influence intracellular signaling (Wnt/Notch/Bone morphogenic protein pathways) and bone mineralization. The osteoblasts and MSCs showed only minor age- and sex-dependent proteomic differences. Finally, the osteoblast and MSC proteomic profiles were altered by ex vivo culture in serum-free media. We conclude that although the proteomic profiles of osteoblasts and MSCs show many similarities, we identified several osteoblast-specific extracellular matrix proteins and an osteoblast-specific intracellular signaling network. Therapeutic targeting of these proteins will possibly have minor effects on MSCs. Furthermore, the use of ex vivo cultured osteoblasts/MSCs in clinical medicine will require careful standardization of the ex vivo handling of the cells.  相似文献   

    

James D. West  Eric D. Austin  Elise M. Rizzi  Ling Yan  Harikrishna Tanjore  Amber L. Crabtree  Christy S. Moore  Gladson Muthian  Erica J. Carrier  David A. Jacobson  Rizwan Hamid  Peggy L. Kendall  Susan Majka  Anandharajan Rathinasabapathy 《International journal of molecular sciences》2021,22(9)

Loss of function KCNK3 mutation is one of the gene variants driving hereditary pulmonary arterial hypertension (PAH). KCNK3 is expressed in several cell and tissue types on both membrane and endoplasmic reticulum and potentially plays a role in multiple pathological process associated with PAH. However, the role of various stressors driving the susceptibility of KCNK3 mutation to PAH is unknown. Hence, we exposed kcnk3^fl/fl animals to hypoxia, metabolic diet and low dose lipopolysaccharide (LPS) and performed molecular characterization of their tissue. We also used tissue samples from KCNK3 patients (skin fibroblast derived inducible pluripotent stem cells, blood, lungs, peripheral blood mononuclear cells) and performed microarray, immunohistochemistry (IHC) and mass cytometry time of flight (CyTOF) experiments. Although a hypoxic insult did not alter vascular tone in kcnk3^fl/fl mice, RNASeq study of these lungs implied that inflammatory and metabolic factors were altered, and the follow-up diet study demonstrated a dysregulation of bone marrow cells in kcnk3^fl/fl mice. Finally, a low dose LPS study clearly showed that inflammation could be a possible second hit driving PAH in kcnk3^fl/fl mice. Multiplex, IHC and CyTOF immunophenotyping studies on human samples confirmed the mouse data and strongly indicated that cell mediated, and innate immune responses may drive PAH susceptibility in these patients. In conclusion, loss of function KCNK3 mutation alters various physiological processes from vascular tone to metabolic diet through inflammation. Our data suggests that altered circulating immune cells may drive PAH susceptibility in patients with KCNK3 mutation.  相似文献   

    

Sakthi Lenin  Elise Ponthier  Kaitlin G. Scheer  Erica C. F. Yeo  Melinda N. Tea  Lisa M. Ebert  Mariana Oksdath Mansilla  Santosh Poonnoose  Ulrich Baumgartner  Bryan W. Day  Rebecca J. Ormsby  Stuart M. Pitson  Guillermo A. Gomez 《International journal of molecular sciences》2021,22(9)

Glioblastoma is one of the most common and lethal types of primary brain tumor. Despite aggressive treatment with chemotherapy and radiotherapy, tumor recurrence within 6–9 months is common. To overcome this, more effective therapies targeting cancer cell stemness, invasion, metabolism, cell death resistance and the interactions of tumor cells with their surrounding microenvironment are required. In this study, we performed a systematic review of the molecular mechanisms that drive glioblastoma progression, which led to the identification of 65 drugs/inhibitors that we screened for their efficacy to kill patient-derived glioma stem cells in two dimensional (2D) cultures and patient-derived three dimensional (3D) glioblastoma explant organoids (GBOs). From the screening, we found a group of drugs that presented different selectivity on different patient-derived in vitro models. Moreover, we found that Costunolide, a TERT inhibitor, was effective in reducing the cell viability in vitro of both primary tumor models as well as tumor models pre-treated with chemotherapy and radiotherapy. These results present a novel workflow for screening a relatively large groups of drugs, whose results could lead to the identification of more personalized and effective treatment for recurrent glioblastoma.  相似文献