Multi-objective evolutionary optimization for greywater reuse in municipal sewer systems

Sustainable design and implementation of greywater reuse (GWR) has to achieve an optimum compromise between costs and potable water demand reduction. Studies show that GWR is an efficient tool for reducing potable water demand. This study presents a multi-objective optimization model for estimating the optimal distribution of different types of GWR homes in an existing municipal sewer system. Six types of GWR homes were examined. The model constrains the momentary wastewater (WW) velocity in the sewer pipes (which is responsible for solids movement). The objective functions in the optimization model are the total WW flow at the outlet of the neighborhoods sewer system and the cost of the on-site GWR treatment system. The optimization routing was achieved by an evolutionary multi-objective optimization coupled with hydrodynamic simulations of a representative sewer system of a neighborhood located at the coast of Israel. The two non-dominated best solutions selected were the ones having either the smallest WW flow discharged at the outlet of the neighborhood sewer system or the lowest daily cost. In both solutions most of the GWR types chosen were the types resulting with the smallest water usage. This lead to only a small difference between the two best solutions, regarding the diurnal patterns of the WW flows at the outlet of the neighborhood sewer system. However, in the upstream link a substantial difference was depicted between the diurnal patterns. This difference occurred since to the upstream links only few homes, implementing the same type of GWR, discharge their WW, and in each solution a different type of GWR was implemented in these upstream homes. To the best of our knowledge this is the first multi-objective optimization model aimed at quantitatively trading off the cost of local/onsite GW spatially distributed reuse treatments, and the total amount of WW flow discharged into the municipal sewer system under unsteady flow conditions.     

Influence of rootstock and scion on antioxidant capacity of juice from new pomelo and mandarin varieties

BACKGROUND: New pomelo and mandarin scion varieties growing under subtropical arid conditions were investigated for total antioxidants in the juice. Four different rootstocks—sour orange (Citrus aurantium (L.); SO), Volkamer lemon (C. volkameriana (Ten & Pasq.)), SB812 (C. sunki (Hort. ex Tan.) × Poncirus trifoliate (L.)) and C. macrophylla (Wester)—were tested. RESULTS: Fruit juice was tested for total antioxidants, ascorbic acid and total polyphenol contents. Cyclic voltammetry (CV) analysis revealed three different antioxidant groups for pomelo and four groups for mandarin. CV analysis for the pomelo scion/rootstock combinations showed that ascorbic acid concentration was highest for scions grafted onto SO. Total polyphenol levels were similar among the pomelo varieties. Mandarin analysis revealed that ‘Merav’ scion/SO had the highest ascorbic acid concentration (1.91 ± 0.01 mmol L^?1). Total polyphenol analysis discovered that ‘Merav’ 4/119‐/SB812 gave the highest levels, while ‘Ora Shani’ scion grafted on to any tested rootstock contained the lowest CONCLUSION: We suggest that the SO rootstock is superior to Volka, 812 and macrophylla in terms of juice antioxidant capacity. For the pomelo, both ‘Flamingo’ scions were found to have high antioxidant production capacity, with ‘Flamingo’ 3/73 being slightly superior regardless of the rootstock. The best mandarins were ‘Merav’ hybrids in combination with SO rootstock. Copyright © 2009 Society of Chemical Industry     

Cytoplasmic polyadenylation controls cdc25B mRNA translation in rat oocytes resuming meiosis

Resumption of meiosis in oocytes represents the entry into M-phase of the cell cycle and is regulated by the maturation-promoting factor (MPF). Activation of MPF is catalyzed by the dual specificity phosphatase, cdc25. In mammals, cdc25 is represented by a multigene family consisting of three isoforms: A, B and C. A recent report that female mice lacking cdc25B exhibit impaired fertility suggests a role for this isoform in regulating the G2- to M-transition in mammalian oocytes. Supporting the above-mentioned observation, we demonstrate herein that microinjection of neutralizing antibodies against cdc25B interfered with the ability of rat oocytes to undergo germinal vesicle breakdown (GVB). We also show accumulation of cdc25B in GVB oocytes and a transient reduction in its amount at metaphase I of meiosis. The accumulation of cdc25B was associated with its mRNA cytoplasmatic polyadenylation and was prevented by the protein synthesis inhibitor cyclohexamide as well as by the polyadenylation inhibitor cordycepin. Immunofluorescence staining revealed translocation of cdc25B to the metaphase II spindle apparatus. Taken together, our findings provide evidence that cdc25B is involved in resumption of meiosis in rat oocytes. We further demonstrate for the first time, a periodic accumulation of cdc25B throughout meiosis that is translationally regulated and involves cdc25B mRNA polyadenylation.     

Fuzzy-logic optical optimization of mainframe CPU and memory

The allocation of CPU time and memory resources is a familiar problem in organizations with a large number of users and a single mainframe. Usually the amount of resources allocated to a single user is based on the user's own statistics not on the statistics of the entire organization, therefore patterns are not well identified and the allocation system is prodigal. A fuzzy-logic-based algorithm to optimize the CPU and memory distribution among users based on their history is suggested. The algorithm works on heavy and light users separately since they present different patterns to be observed. The result is a set of rules generated by the fuzzy-logic inference engine that will allow the system to use its computing ability in an optimized manner. Test results on data taken from the Faculty of Engineering of Tel Aviv University demonstrate the capabilities of the new algorithm.     

Small Molecule Receptor Binding Inhibitors with In Vivo Efficacy against Botulinum Neurotoxin Serotypes A and E

Botulinum neurotoxins (BoNTs) are the most poisonous substances in nature. Currently, the only therapy for botulism is antitoxin. This therapy suffers from several limitations and hence new therapeutic strategies are desired. One of the limitations in discovering BoNT inhibitors is the absence of an in vitro assay that correlates with toxin neutralization in vivo. In this work, a high-throughput screening assay for receptor-binding inhibitors against BoNT/A was developed. The assay is composed of two chimeric proteins: a receptor-simulating protein, consisting of the fourth luminal loop of synaptic vesicle protein 2C fused to glutathione-S-transferase, and a toxin-simulating protein, consisting of the receptor-binding domain of BoNT/A fused to beta-galactosidase. The assay was applied to screen the LOPAC1280 compound library. Seven selected compounds were evaluated in mice exposed to a lethal dose of BoNT/A. The compound aurintricarboxylic acid (ATA) conferred 92% protection, whereas significant delayed time to death (p < 0.005) was observed for three additional compounds. Remarkably, ATA was also fully protective in mice challenged with a lethal dose of BoNT/E, which also uses the SV2 receptor. This study demonstrates that receptor-binding inhibitors have the potential to serve as next generation therapeutics for botulism, and therefore the assay developed may facilitate discovery of new anti-BoNT countermeasures.     

Control of the Aero‐Electric Power Station—an exciting QFT application for the 21st century

The Aero‐Electric Power Station is the ultimate solar power station, utilizing the dry, hot air of Earth's desert zones. By spraying water at the top of e.g. a 1200 m tall chimney with a diameter of 400 m, the air is cooled by evaporation and flows downwards through turbines at the bottom, generating 380 MW of net electric power. The Aero‐Electric Power Station is still in the planning stage, and this paper belongs to a long series of feasibility studies. The current ‘truth’ model of the Aero‐Electric Power Station is a one‐dimensional partial differential equation model. The external slowly changing weather, defined as the mean air pressures, temperatures and humidity at the top and bottom of the tower, determines the optimal operating point, i.e. the optimal water spray flow and turbine velocity that give the largest net power. The gross power produced by the turbine is partly delivered to the grid and partly to pump sea water to spray water reservoirs. The reservoirs make it possible to use the pumping power and the spray flow rate as control. Wind changes cause significant deviations from the mean external air pressures, requiring closed loop regulation to keep the rotor velocity constant. The Aero‐Electric Power Station may be modelled as an uncertain, unstable irrational transfer function, with two disturbances (external air pressure deviations at top and bottom), two control variables (turbine power and spray flow), and one output (rotor velocity), without a cascaded structure, giving rise to a robust load sharing control problem. A robust linear feedback regulator is designed by QFT, in such a way that the load of regulation is shared between the two control inputs. A closed loop step response simulation for one operating condition, using the ‘truth’ model, demonstrates the design. Copyright © 2003 John Wiley & Sons, Ltd.     

Bicoherence analysis of new cardiovascular spectral components observed in heart-transplant patients: statistical approach for bicoherence thresholding

Following heart transplant (HT), the patient's heart functions under complete cardiac denervation. As a result, the variability in physiologic signals is extremely reduced. We have previously reported that in addition to the typical spectral components (of very low amplitude), part of the HT patients (above 50%) demonstrated unexpected additional peaks in their heart rate and blood pressure spectra. These peaks may be a result of the development of compensatory mechanism induced by loss of parasympathetic control, or of increased importance of nonlinear control interactions. It is important to quantify these strange, unexpected very-high frequency (VHF) peaks, to understand their origin and their contribution to cardiac control in transplant patients. In this paper, we chose to examine these VHF peaks by applying the bicoherence approach. The reduced signal to noise ratio, occurring in these patients, results, however, in an extremely noisy bicoherence. We, therefore, developed several statistical tools in order to distinguish between "true" bicoherence peaks (reflecting true phase coupling) and spurious peaks. The outcome of these methods was an efficient and sensitive bicoherence thresholding procedure, able to identify most of the spurious peaks. Applying these tools to the bicoherence of cardiovascular signals which display VHF peaks, revealed several significant bicoherence peaks. Interestingly, these peaks consisted of two different types. The first type of VHF peaks simply reflects nonlinear cardiac-respiratory coupling, imposed by nonsinusoidal breathing. The second type, however, is clearly not induced by the respiratory system. We believe that these type-2 VHF peaks reflect the evolution of a new, yet unexplained, compensatory mechanism.     

Silver halide photonic crystal fibers for the middle infrared

Photonic crystal fibers are normally holey silica fibers, which are opaque in the mid- and far-infrared. We have fabricated novel fibers by multiple extrusions of silver halide crystalline materials, which are highly transparent in the mid-infrared. These fibers are composed of two solid materials; The core consists of pure AgBr, and the cladding includes AgCl fiberoptic elements arranged in two concentric hexagonal rings around the core. Flexible fibers of outer diameter 1 mm and length of approximately 1 m were fabricated, and their optical properties were measured. These fibers exhibited core-clad behavior and would be extremely useful for IR laser power transmission, IR radiometry, and IR spectroscopy.