External and internal calibration of the MHC class I-specific receptor Ly49A on murine natural killer cells

Expression of the H-2Dd-specific inhibitory receptor Ly49A on murine NK cells is subject to MHC class I-dependent modulation in vivo. As a result, NK cells in H-2Dd-transgenic mice express low cell surface levels of Ly49A, whereas NK cells from nontransgenic C57BL/6 (B6) mice express high levels. The purpose of this study was to assess the role of MHC class I molecules on the NK cell itself vs those on surrounding cells in this calibration and to test whether the Ly49A levels are subject to regulation in mature NK cells also. Analysis of transgenic mice with mosaic expression of an H-2Dd/Ld transgene showed that MHC class I molecules on surrounding cells (external ligands) and on the NK cell itself (internal ligands) played distinct roles in the determination of Ly49A levels. External ligands were involved in down-regulation of Ly49A levels in vivo, whereas internal ligands kept the down-regulated levels of Ly49A low upon NK cell activation in vitro. Furthermore, in an experimental system based on adoptive transfer of spleen cells, receptor down-regulation of Ly49A occurred as a rapid adaptation process in mature NK cells after interaction with the H-2Dd ligand in vivo. This suggests that Ly49 levels are not fixed but can be changed in mature NK cells when they are exposed to a changed MHC class I environment.     

Pressureless sintering of Y2O3-CeO2-doped sialons

Various sialon materials have been prepared by pressureless sintering at 1775 and 1825 °C using Y₂O₃ and/or Ce0₂ as sintering aids. Constant molar amounts of the oxide mixtures were added in the ratios Y₂O₃/CeO₂: 100/0, 75/25, 50/50, 25/75, 0/100 corresponding to 6.0 and 9.25 wt% for the pure Y₂O₃ and pure CeO₂, respectively. Only one of the compositional series reached full density at 1775 °C with cerium replacing yttrium, whereas at 1825 °C all compositional series except one became dense. The samples sintered showed that yttrium but not cerium stabilizes the sialon phase in these ceramics. The dense cerium-sialon ceramics sintered at 1825 °C have as good hardness and indentation fracture toughness as the corresponding yttrium-sialon ceramics, or even higher for the sialon type of materials. For the mixed - sialon materials the hardness decreased as the amount of a sialon phase decreased by increasing cerium-doping.     

Production of rapidly solidified Al/SiC composites

Rapidly solidified metal matrix composites have been produced on a laboratory scale either by (1) melt spinning a composite after introduction of the ceramic phase and extrusion of the flakes obtained, or (2) blending melt-spun powder (basic alloy) with the ceramic phase and subsequent extrusion. AlMg(Si) alloys were used as matrix material while SiC particles with diameters of 3 or 20 m were used as the ceramic phase. For the composites prepared by route 1 it was found that the basic alloy was reinforced by the addition of 3 m particles whereas for the 20m particles reinforcement was observed only for very ductile matrices. The bond between SiC particles and matrix was good. A diffusion and wetting bond was formed. For the composites prepared by route 2 it was found that reinforcement did not occur and that the bond between particles and matrix was weak. Debonding of the particles took place in the case of tensile fracture. The advantage of a rapidly solidified matrix for these composites is that relatively high ductilities are combined with good reinforcement effects. Prior contact of the ceramic phase and the aluminium melt is needed for a good bond between SiC and the matrix material. It is concluded that route 1 should be preferred for the production of rapidly solidified aluminium matrix composites.     

Fourier Transform Spectrometer for the 1 to 10mm region

A Fourier Transform Spectrometer using a 250 Watt Mercury lamp, a lamellar grating and a pumped He-cooled detector is applied to the millimeter wave spectral region. The pure rotation spectrum of CH₂F, the transmission of graphite particles in a cloud chamber, the interference on round copper dots and the absorption of iron whiskers have been observed.     

The effect of appraisers in estimating metabolic rate with the Edholm scale

A study was made of the appraisers' effect on the estimation of metabolic rate with the Edholm scale and a table of the ISO 7243 heat stress standard. The appraisers, five experienced and five inexperienced persons, estimated the metabolic rate of three different work tasks from videotapes. Analysis of variance indicated significant ( [Formula: see text] ) differences in the appraisers' recordings of the activities. The appraisers were grouped according to the similarity of the estimated values they gave. The groups thus contained both experienced and inexperienced appraisers, and it was not possible to classify the appraisers into experienced and inexperienced groups according to their earlier experience. The metabolic rates according to the Edholm scale were higher than according to the ISO 7243 table. The differences in metabolic rates given by the individual observers varied from 38 to 118 W/m(2). The variations in the estimation of metabolic rates were greater when the Edholm scale was used. This variation caused considerable variation also in the predicted mean vote, PMV index. It is recommended that the appraisers be selected carefully, because it is not possible to know whether a randomly selected appraiser is an 'average' or an 'extreme' appraiser without a test. Before conducting extensive field surveys where several appraisers estimate the metabolic rates, it would be useful to arrange training in order to calibrate the levels of the Edholm scale as well as ISO method among the appraisers because training clearly unified the estimation.     

Variation in light scattering and water-holding capacity along the porcine Longissimus dorsi muscle

The meat quality of M. longissimus muscle was evaluated in 851 pigs by using the Fibre Optic Probe (FOP) at three sites in the muscle and in both halves of the carcass. A systematic difference between sites was found, with the lowest light scattering (indicating the best meat quality) in the mid-part of the muscle and higher light scattering in the anterior and posterior parts. A non-systematic variation was also observed, with the opposite pattern in some animals, even though it was not frequent. A negative influence of the shackling was found, yielding higher FOP values in the shackled half.

Drip loss measurements in the Longissimus muscle, taken from another 100 pig carcasses, were evaluated using three methods. Drip loss, too, showed a considerable variation along the Longissimus muscle, with minimum losses around the last rib. Repeatability estimates, calculated from two non-consecutive pieces of the Longissimus dorsi muscle from each carcass, varied from 0·4 when keeping samples vacuum packed for 2 days, to 0·5 when the samples were either kept in plain plastic bags for 2 days or in a meat container with a squared inset for 1 or 2 days.     

Development of Cr and Al Pack Cementation Coatings on Co-Based γ/γ′ Superalloys

Metallurgical and Materials Transactions A - Co-based superalloys have been developed as candidate materials to replace Ni-based superalloys in hot sections of turbine engines, however, their...     

The action of various lime soap dispersants

The course of the lime soap-dispersing action of various surfactants has been examined by use of a titrimetric method. Two types of dispersants can be distinguished. With the addition of a surfactant of the first type, the concentration of the dispersed fatty acid increases gradually. If a surfactant of the second type is used, a definite increase of the fatty acid concentration first occurs after the addition of considerable amounts of the dispersant. Ethylene oxide adducts and sodium triethylene glycol monolauryl ether sulfate behave like dispersants of the first type whereas sodium dodecylbenzenesulphonate and sodium lauryl sulfate belong to those of the second type. The dispersing action of both types of surfactants is discussed.     

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