Extracellular vesicles (EVs) are a family of particles/vesicles present in blood and body fluids, composed of phospholipid bilayers that carry a variety of molecules that can mediate cell communication, modulating crucial cell processes such as homeostasis, induction/dampening of inflammation, and promotion of repair. Their existence, initially suspected in 1946 and confirmed in 1967, spurred a sharp increase in the number of scientific publications. Paradoxically, the increasing interest for EV content and function progressively reduced the relevance for a precise nomenclature in classifying EVs, therefore leading to a confusing scientific production. The aim of this review was to analyze the evolution of the progress in the knowledge and definition of EVs over the years, with an overview of the methodologies used for the identification of the vesicles, their cell of origin, and the detection of their cargo. The MISEV 2018 guidelines for the proper recognition nomenclature and ways to study EVs are summarized. The review finishes with a “more questions than answers” chapter, in which some of the problems we still face to fully understand the EV function and potential as a diagnostic and therapeutic tool are analyzed. 相似文献
L. Markson and P. Bloom (1997) concluded that there was evidence against a dedicated system for word learning on the basis of their finding that children remembered a novel word and a novel fact equally well. However, a word-learning system involves more than recognition memory; it must also provide a means to guide the extension of words to additional exemplars, and words and facts may differ with regard to extendibility. Two studies are reported in which 2–4-year-old children learned novel words and novel facts for unfamiliar objects and then were asked to extend the words and facts to additional exemplars of the training objects. In both studies, children extended the novel word to significantly more category members than they extended the novel fact. The results show that by 2 years of age, children honor the necessary extendibility of novel count nouns but are uncertain about the extendibility of arbitrary facts. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
The authors present a comprehensive analysis of the chromatic dispersion effects in harmonic upconverted millimeter-wave (mm-wave) fiber-optic links. The optical upconversion is performed through a photonic mixer based on a Mach-Zehnder electrooptical modulator. It is shown that by biasing the electrooptical modulator either at the maximum or at the minimum transmission bias points, the dispersion-induced power penalty effect on the upconverted signal may be sharply mitigated, which results in increasing the frequency-length product of the fiber-optic link. Experimental results are provided for the three different types of bias 相似文献
Recent research has indicated that short term administration of glycine propionyl-L-carnitine (GPLC) significantly elevates
levels of nitric oxide metabolites at rest and in response to reactive hyperaemia. However, no scientific evidence exists
that suggests such supplementation enhances exercise performance in healthy, trained individuals. The purpose of this study
was to examine the effects of GPLC on the performance of repeated high intensity stationary cycle sprints with limited recovery
periods in resistance trained male subjects. 相似文献
Burrowing mayfly species of the genus Hexagenia are well known indicators of environmental health in lakes and rivers. Two species, H. limbata and H. rigida, are indistinguishable as nymphs and as adult females. Our objectives were to develop a genetic technique to distinguish between the two species and identify morphological features that separate cryptic nymphs and adult females. Fifty nymphs were collected before emergence from 10 sites throughout the western basin of Lake Erie in 2004 and 2005. Using known specimens of adult aerial male H. limbata and H. rigida, we used the mitochondrial cytochrome c oxidase subunit 1 (COI) gene to identify a 16 base pair (bp) difference between species. DNA sequencing confirmed correct species identification based on differences in abdominal pigmentation patterns on adult female imagos in 19 of 20 cases; the lone exception was a female with very faint pigmentation. Pigmentation patterns between species were consistent on nymphs, subimagos and imagos of both sexes. Populations of both species are panmictic across the western basin of Lake Erie, but H. limbata is the numerically dominant species, representing 70 to 100% of nymphs at sites in both years. A separate lineage of H limbata was discovered in the samples. The ability to distinguish nymphs of the two species will aid in developing more sensitive ecosystem indicators. 相似文献
Vibration-based damage detection methods have become widely used because of their advantages over traditional methods. This paper presents a new approach to identify the crack depth in steel beam structures based on vibration analysis using the Finite Element Method (FEM) and Artificial Neural Network (ANN) combined with Butterfly Optimization Algorithm (BOA). ANN is quite successful in such identification issues, but it has some limitations, such as reduction of error after system training is complete, which means the output does not provide optimal results. This paper improves ANN training after introducing BOA as a hybrid model (BOA-ANN). Natural frequencies are used as input parameters and crack depth as output. The data are collected from improved FEM using simulation tools (ABAQUS) based on different crack depths and locations as the first stage. Next, data are collected from experimental analysis of cracked beams based on different crack depths and locations to test the reliability of the presented technique. The proposed approach, compared to other methods, can predict crack depth with improved accuracy. 相似文献
Herein we wish to report a new non‐enzymatic kinetic resolution of racemic 4‐formyl[2.2]paracyclophane based on Noyori asymmetric transfer hydrogenations (KR‐ATH). Our approach, which provides an efficient access to enantiopure (Rp)‐ and (Sp)‐4‐formyl[2.2]paracyclophane (>99% ee, 39% and 41% isolated yields, respectively), is operationally simple and can be run on the gram‐scale thus confirming the practical applicability of this method.
Aligned multi-walled carbon nanotube forests were grown by chemical vapour deposition on carbon fibers by the use of an amorphous Si interface. The Si layer creates a barrier, hindering the Fe catalyst diffusion into the carbon fibers. This method provides a way to tailor the thermal, electrical and mechanical properties of the fiber-resin interface of a polymer composite. 相似文献