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通过调查得知,包括虚拟现实技术在机床制造中应用可能性的应用方法、策略和工具在产品开发中的现状.对调查结果进行了讨论,考虑到研究理论的可移植性和对企业内部实战的依赖性.讨论的结果为基础研究领域的命题"基于职能单位的产品开发"提供结论. 相似文献
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Paul J. Steiner Matthew A. Bedewitz Angélica V. Medina-Cucurella Sean R. Cutler Timothy A. Whitehead 《American Institute of Chemical Engineers》2020,66(3):e16767
Small-molecule biosensors have major applications in biotechnology and medicine but remain difficult to engineer. Plant hormone receptors represent an attractive platform for engineering such biosensors because their chemically induced dimerization architectures naturally decouple small-molecule sensing and sensor actuation. Rapid biosensor engineering will require quantitative high-throughput screening methods. Here we develop a yeast surface display (YSD) platform for the PYR1/HAB1 abscisic acid sensor of Arabidopsis thaliana. We extensively optimized PYR1 surface display, HAB1 purification, and binding reaction conditions. Our system reproduces previous results with wild-type and engineered receptors, and a mathematical analysis of the PYR1/HAB1 system allows us to infer all binding constants. Critically, we find that a previously engineered PYR1 receptor with altered ligand specificity binds HAB1 with identical affinity, suggesting that substantial reengineering of the PYR1 binding pocket does not compromise sensor actuation. This YSD platform for A. thaliana PYR1/HAB1 will facilitate future biosensor engineering efforts. 相似文献
55.
A 5-cell solid oxide fuel cell stack was tested during 10,000 h of continuous operation with simulated reformate gas as fuel (71 vol.% H2, 20.7 vol.% CO2 and 8.3 vol.% steam) under high fuel utilization (73%) and constant current load (0.5 A cm?2 or 25 A) at 750 °C. In situ electrochemical impedance spectroscopy was used to monitor the evolution of ohmic and polarisation resistances of individual cells in the stack without interrupting the current load. Impedance spectra were recorded on each cell periodically (every 1000 h) or after uncontrolled incidents happened with the test setup. It has been found that the stack degradation is mainly attributed to the increased ohmic resistance, pointing to possible causes such as interconnect corrosion and reduced effective contact areas between cells and interconnects. The degradation rate during the first 5000 h was about 1% kh?1, but increased afterwards up to 1.5% kh?1 due to the impact of incidents. Both types of incidents (fuel supply fluctuations and overloading failure of the electronic load) were complicated by inhomogeneous fuel distribution among cells, leading to most probably partial re-oxidation of the anode, accelerating the stack degradation. 相似文献
56.
Hahn Griebel W. Schreiber Carls J. Großfeld Steinbeck Beckel Schwaibold Lehnartz Meumann Schormüller Jesser R. Strohecker Lehnartz Diemair R. Grau Lars Erlandson Patzsch Reichard Ph. Bruno Roßmann Brüning Karl Sauer Willy Lindner Kanitz O. Windhausen Schloemer Amelung M. Steiner R. Mancke K. Höll Goreczky E. Tornow Zacher Kleinhans Bäurle 《European Food Research and Technology》1943,85(1):70-112
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Schwaibold Schormüller Beckel W. Sutthoff Lars Erlandsen v. Skramlik W. Schreiber Kanitz Bierotte M. Steiner W. Wodsak G. Steinhoff H. J. Steinbeck Frey-Wyssling M. Gordienko K. Dirr Pawletta Stelzer W. Wodsak W. Bartels Hoepke G. Stamm Brüning W. Hämmerle Schnetka K. Felix H. Schülke Kluge Krzywanek W. Brandt Gertraude Körner-Hennig K. J. Demeter Damm J. Ph. Bruno Roßmann W. Karlowa H. Ketterl L. W. Jirak W. Preiß Diemair W. Ludorff Jahr Griebel J. J. van Eck W. Preiß C. Pyriki K. Müller Jesser K. Boresch Fr. Bartschat 《European Food Research and Technology》1939,77(3):290-336
58.
Bellare M. Garay J.A. Hauser R. Herzberg A. Krawczyk H. Steiner M. Tsudik G. Van Herreweghen E. Waidner M. 《Selected Areas in Communications, IEEE Journal on》2000,18(4):611-627
This paper discusses the design, implementation, and deployment of a secure and practical payment system for electronic commerce on the Internet. The system is based on the iKP family of protocols-(i=1,2,3)-developed at IBM Research. The protocols implement credit card-based transactions between buyers and merchants while the existing financial network is used for payment clearing and authorization. The protocols are extensible and can be readily applied to other account-based payment models, such as debit cards. They are based on careful and minimal use of public-key cryptography, and can be implemented in either software or hardware. Individual protocols differ in both complexity and degree of security. In addition to being both a precursor and a direct ancestor of the well-known SET standard, iKP-based payment systems have been in continuous operation on the Internet since mid-1996. This longevity-as well as the security and relative simplicity of the underlying mechanisms-makes the iKP experience unique. For this reason, this paper also reports on, and addresses, a number of practical issues arising in the course of implementation and real-world deployment of a secure payment system 相似文献
59.
Recognising the various types of absorbed nitrogen in nitrided iron-based alloys and the lack of accuracy in local composition analysis of the individual nano-sized nitride precipitates (e.g. by atom probe tomography), precise determination of the nitride composition requires so-called denitriding treatments. This study proposes two routes to determine the nanonitride stoichiometry, which are demonstrated by experimental analysis of the denitriding behaviour in hydrogen gas of nitrided, model Fe–V alloys. The minimal nitrogen activity to stabilise the alloying element nitrides MeN in Fe–Me–N alloys is calculated. A strategy is offered to stabilise the alloying element nitrides and to determine the nitrogen content strongly bonded in the nitrides. 相似文献
60.
Forman Susan G.; Fagley N. S.; Steiner Denise Dreitlein; Schneider Kenneth 《Canadian Metallurgical Quarterly》2009,3(4):226
This study examined perceptions of school psychologists regarding their use of evidence-based interventions in school settings after completing a graduate course in school-based psychosocial interventions. Ninety-four individuals who completed the course during the 10 years prior to the study were asked to complete a survey that focused on personal and organizational factors affecting the implementation of evidence-based interventions through assessment of (a) the extent of their use of interventions in professional school psychology practice, (b) perceptions of facilitators to implementation, and (c) perceptions of barriers to implementation. Fifty-four responded (a 57% response rate); analyses were conducted on the responses of 34 individuals who indicated that they were currently school psychologists working in schools. Results indicated that school psychologists’ personal beliefs about the interventions influenced their decisions to implement them. Several evidence-based interventions were less likely to be used in urban practice settings. Training in evidence-based interventions should address the personal and contextual issues that are related to implementation, as well as the procedures of the interventions. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献