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61.
Yixuan Jia Christoph A. Spiegel Alexander Welle Stefan Heißler Elaheh Sedghamiz Modan Liu Wolfgang Wenzel Maximilian Hackner Joachim P. Spatz Manuel Tsotsalas Eva Blasco 《Advanced functional materials》2023,33(39):2207826
Manufacturing programmable materials, whose mechanical properties can be adapted on demand, is highly desired for their application in areas ranging from robotics, to biomedicine, or microfluidics. Herein, the inclusion of dynamic and living bonds, such as alkoxyamines, in a printable formulation suitable for two-photon 3D laser printing is exploited. On one hand, taking advantage of the dynamic covalent character of alkoxyamines, the nitroxide exchange reaction is investigated. As a consequence, a reduction of the Young´s Modulus by 50%, is measured by nanoindentation. On the other hand, due to its “living” characteristic, the chain extension becomes possible via nitroxide mediated polymerization. In particular, living nitroxide mediated polymerization of styrene results not only in a dramatic increase of the volume (≈8 times) of the 3D printed microstructure but also an increase of the Young's Modulus by two orders of magnitude (from 14 MPa to 2.7 GPa), while maintaining the shape including fine structural details. Thus, the approach introduces a new dimension by enabling to create microstructures with dynamically tunable size and mechanical properties. 相似文献
62.
The effects of placement of a miniature implantable stimulator on motor unit recruitment were examined in the posterior head of cat biceps femoris. The implantable stimulator (13-mm long x 2-mm diameter) was injected either proximally near the main nerve branch, or distally near the muscle insertion, through a 12-gauge hypodermic needle. Glycogen-depletion methods were used to map the distribution of fibers activated by electrical stimulation. Muscle fibers were found to be depleted at most or all proximodistal levels of the muscle, but the density of depleted fibers varied transversely according to the stimulus strength and proximity of the device to the nerve-entry site. Thus, muscle cross sections often had a "patchy" appearance produced because different proportions of depleted fibers intermingled with undepleted fibers in different parts of the cross section. In other preparations, the force of muscle contraction was measured when stimuli of varying strengths were delivered by the stimulator positioned at the same proximal or distal sites within the muscle. Devices placed close to the nerve-entry site produced the greatest forces. Those placed more distally produced less force. As stimulus current and/or pulse width increased, muscle force increased, often in steps, until a maximum was reached, which was usually limited by the compliance voltage of the device to less than the force produced by whole nerve stimulation. 相似文献
63.
FJ Vingerhoets I Hegyi A Aguzzi P Myers G Pizzolato T Landis 《Canadian Metallurgical Quarterly》1998,51(2):617-619
A patient with histopathologically verified sporadic Creutzfeldt-Jakob disease (CJD) presented initially with diplopia, sleep disturbances, and L-dopa-responsive parkinsonism. After more than a year of slow progression, he did not become demented, and failed to fulfill the clinical criteria for possible CJD. No clinical examinations currently proposed to detect CJD showed the disease. CJD should be in the differential diagnosis of "parkinson plus" syndromes until a different etiology has been found or a histopathologic examination performed. 相似文献
64.
JD Kasprzak WB Vletter JR Roelandt JR van Meegen R Johnson FJ Ten Cate 《Canadian Metallurgical Quarterly》1998,40(2):314-321
To investigate possible biochemical mechanisms underlying the "toxic gain of function" associated with polyglutamine expansions, the ability of guinea pig liver tissue transglutaminase to catalyze covalent attachments of various polyamines to polyglutamine peptides was examined. Of the polyamines tested, spermine is the most active substrate, followed by spermidine and putrescine. Formation of covalent cross links between polyglutamine peptides and polyamines yields high-M(r) aggregates--a process that is favored with longer polyglutamines. In the presence of tissue transglutaminase, purified glyceraldehyde-3-phosphate dehydrogenase (a key glycolytic enzyme that binds tightly to the polyglutamine domains of both huntingtin and dentatorubral-pallidoluysian atrophy proteins) is covalently attached to polyglutamine peptides in vitro, resulting in the formation of high-M(r) aggregates. In addition, endogenous glyceraldehyde-3-phosphate dehydrogenase of a Balb-c 3T3 fibroblast cell line overexpressing human tissue transglutaminase forms cross-links with a Q60 polypeptide added to the cell homogenate. Possibly, expansion of polyglutamine domains (thus far known to occur in the gene products associated with at least seven neurodegenerative diseases) leads to increased/aberrant tissue transglutaminase-catalyzed cross-linking reactions with both polyamines and susceptible proteins, such as glyceraldehyde-3-phosphate dehydrogenase. Formation of cross-linked heteropolymers may lead to deposition of high-M(r) protein aggregates, thereby contributing to cell death. 相似文献
65.
Avoidance of stomas and delayed anastomosis for bowel necrosis: the ''clip and drop-back'' technique
WG Vaughan JL Grosfeld K West LR Scherer E Villamizar FJ Rescorla 《Canadian Metallurgical Quarterly》1996,31(4):542-545
Necrotizing enterocolitis (NEC) and midgut volvulus (MGV) often are associated with extensive bowel necrosis. These cases may require extensive enterectomy and the formation of high or multiple stomas, and frequently are complicated by short bowel syndrome, excessive fluid losses, fistulas, stenosis, and skin breakdown. This report describes a "clip and drop-back" technique, followed by delayed anastomosis performed 48 to 72 hours later. The technique was successful in five severely ill infants (3 NEC, 2 MGV) with extensive necrosis, bowel perforation(s), and peritonitis, who required either a high stoma near the ligament of Treitz or multiple resections and enterostomies. This method removes obvious necrotic perforated bowel, controls contamination, avoids stomas (and their inherent complications in this age group), and preserves bowel length. All five babies survived. The technique is a useful addition to the pediatric surgeon's operative armamentarium in selective cases. 相似文献
66.
P Usobiaga FJ Medrano M Gasset JL Garciá JL Saiz G Rivas J Laynez M Menéndez 《Canadian Metallurgical Quarterly》1996,271(12):6832-6838
LytA amidase is the best known bacterial autolysin. It breaks down the N-acetylmuramoyl-L-alanine bonds in the peptidoglycan backbone of Streptococcus pneumoniae and requires the presence of choline residues in the cell-wall teichoic acids for activity. Genetic experiments have supported the hypothesis that its 36-kDa chain has evolved by the fusion of two independent modules: the NH2-terminal module, responsible for the catalytic activity, and the COOH-terminal module, involved in the attachment to the cell wall. The structural organization of LytA amidase and of its isolated COOH-terminal module (C-LytA) and the variations induced by choline binding have been examined by differential scanning calorimetry and analytical ultracentrifugation. Deconvolution of calorimetric curves have revealed a folding of the polypeptide chain in several independent or quasi-independent cooperative domains. Elementary transitions in C-LytA are close but not identical to those assigned to the COOH-terminal module in the complete amidase, particularly in the absence of choline. These results indicate that the NH2-terminal region of the protein is important for attaining the native tertiary fold of the COOH terminus. Analytical ultracentrifugation studies have shown that LytA exhibits a monomer <--> dimer association equilibrium, through the COOH-terminal part of the molecule. Dimerization is regulated by choline interaction and involves the preferential binding of two molecules of choline per dimer. Sedimentation velocity experiments give frictional ratios of 1.1 for C-LytA monomer and 1.4 for C-LytA and LytA dimers; values that deviated from that of globular rigid particles. When considered together, present results give evidence that LytA amidase might be described as an elongated molecule consisting of at least four domains per subunit (two per module) designated here in as N1, N2, C1, and C2. Intersubunit cooperative interactions through the C2 domain in LytA dimer occur under all experimental conditions, while C-LytA requires the saturation of low affinity choline binding sites. The relevance of the structural features deduced here for LytA amidase is examined in connection with its biological function. 相似文献
67.
C van den Bogaard HJ van den Hoogen FJ Huygen C van Weel 《Canadian Metallurgical Quarterly》1993,25(7):471-475
BACKGROUND: Previous studies reported that breast-feeding protects children against a variety of diseases, but these studies were generally conducted on "high-risk" or hospitalized children. This paper describes the results of our study on the effects of breast-feeding on rate of illness in normal children with a family history of atopy. METHODS: A historic cohort approach of 794 children with a family history of atopy was used to assess the effects of breast-feeding on illness rates. Family history of atopy was based on allergic diseases in family members as registered by the family physician. Illness data from birth onwards were available from the Continuous Morbidity Registration of the Department of Family Medicine. Information on breast-feeding was collected by postal questionnaire. We then compared rates of illness between children with a family history of atopy who were and who were not breast-fed. RESULTS: Breast-feeding was related to lower levels of childhood illness both in the first and the first three years of life. In the first year of life they had fewer episodes of gastroenteritis, lower respiratory tract infections, and digestive tract disorders. Over the next three years of life they had fewer respiratory tract infections and skin infections. CONCLUSIONS: Our results suggest a protective effect of breast-feeding among children with a family history of atopy that is not confined to the period of breast-feeding but continues during the first three years of life. Breast-feeding should be promoted in children with a family history of atopy. 相似文献
68.
FJ Entin 《Canadian Metallurgical Quarterly》1982,39(8):1324-1328
The process of preparing and reviewing a contract for a pharmacy computer system is described. The purpose of the contract is to establish rules governing the relationship between the vendor and the buyer. It should identify clearly the performance expectations of both parties. The contract negotiating process also allows each party to gain insight to the other's motives by way of responses to proposals for specific contract inclusions. Generally, the buyer should be wary of: (1) a vendor who insists that his form contract be signed without modification; (2) signing more than one contract with the same vendor; (3) vague, nonquantifiable performance criteria; (4) "best-efforts" warrantees; (5) an expectation that difficulties with the vendor can be solved if the remedy is not in the contract; and (6) acceptance upon delivery. The buyer should strive for: (1) a complete contract reflecting the nature of the business deal negotiated, (2) specific performance criteria, (3) warrantees of performance with meaningful remedies, and (4) payments tied to performance. The most important provision of the contract is the one that makes payment to the vendor contingent upon the actual demonstration of results. 相似文献
69.
H. Saito M.F. Bellan A. Al-Habshi M. Aizpuru F. Blasco 《International journal of remote sensing》2013,34(21):4073-4092
Mangroves reach their optimal development in the wet tropics although some little known mangrove stands are reported in subtropical arid coastlines especially from the Red Sea to Pakistan where they form one of the driest mangrove habitats in the world. Because they constitute the only available evergreen forest in hyper-arid warm coastal areas, the main wetlands for migratory birds and essential nursery ground for many species of fish, it is imperative to produce a sufficiently accurate map for monitoring their changes and for their protection. The main objective of the present work is to test and to select the best methodological approach to discriminate and map the mangroves and related coastal ecosystems in the United Arab Emirates (UAE), between Abu Dhabi and Dubai, a coastal stretch about 750?km long. It was found that the best practical results were produced by the Maximum Likelihood and Mahalanobis classifications although some limitations remain unsolved, especially in open ecosystems, which are common in arid areas. Moreover, SPOT-4 High-Resolution Visible Infra-Red (HRVIR) data proves at least as efficient as TERRA ASTER data, in spite of a slightly finer ground resolution and the great number of channels for ASTER. Ultimately, the most appropriate working scale for mapping coastal habitats, compatible with pixel size, is in the order of 1/25 000. 相似文献
70.
Miguel A Rosales Luis M Cervilla Eva Sánchez‐Rodríguez María del Mar Rubio‐Wilhelmi Begoña Blasco Juan J Ríos Teresa Soriano Nicolás Castilla Luis Romero Juan M Ruiz 《Journal of the science of food and agriculture》2011,91(1):152-162
BACKGROUND: The aim of this study was to examine how different environmental factors (temperature, solar radiation, and vapour‐pressure deficit [VPD]) influenced nutritional quality and flavour of cherry tomato fruits (Solanum lycopersicum L. cv. Naomi) grown in two types of experimental Mediterranean greenhouses: parral (low technology) and multispan (high technology). RESULTS: Fruits were sampled three times during 3 years (2004, 2005 and 2006): at the beginning, middle and end of the fruit production period. Values for temperature, solar radiation, and VPD peaked in the third sampling in both greenhouses; values were higher in the parral‐type greenhouse, triggering abiotic stress. This stress reduced the accumulation of lycopene and essential elements, augmenting the phytonutrient content and the antioxidant capacity of tomatoes. During the third sampling, sugars were increased while organic acid content diminished, producing tomatoes with a sweeter‐milder flavour. The parral greenhouse produced tomatoes with higher phenolic compounds and ascorbic acid contents, together with a greater antioxidant capacity, without showing differences in flavour parameters. CONCLUSION: The higher phytonutrients content and antioxidant activity during the environmental stress, more pronounced in parral than multispan greenhouse, together with the sweeter‐milder flavour, conferred a notable nutritional benefit, which considerably improved the nutritional and organoleptic quality of these tomatoes. Copyright © 2010 Society of Chemical Industry 相似文献