Social desirability and responses to items from three MMPI scales: D, Sc, and K.

For random samples of 25 D and 32 Sc items, and for 29 of the 30 K items, ratings of social desirability were correlated with the probabilities that the items would be endorsed when the MMPI was used as a personality test (based on proportion of a college student sample actually endorsing each item). The correlations were .82 and .89 for the D and Sc items respectively. For the K-scale items, correlations of social desirability were computed with endorsement probabilities from a "typical college sample" (r = .50), and "Average-K" group (r = .38), and a "high-K" group (r = .66). "The results were interpreted as demonstrating the validity of K as a measure of the set to respond to items in terms of their social desirability." (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Eysenck's tendermindedness dimension: a critique.

An evaluation of Eysenck's research on his tough-mindedness-tender-mindedness factor indicates that this dimension is based not upon fact, but rather rests on "… miscalculations and a disregard for a significant portion of his data. It conceals rather than reveals the attitudinal differences existing among political groups." (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Derivatization of surface-bound peptides for mass spectrometric detection via threshold single photon ionization

Chemical derivatization of peptides allows efficient F2 laser single photon ionization (SPI) of Fmoc-derivatized peptides covalently bound to surfaces. Laser desorption photoionization mass spectrometry using 337-nm pulses for desorption and 157.6-nm pulses for threshold SPI forms large ions identified as common peptide fragments bound to either Fmoc or the surface linker. Electronic structure calculations indicate the Fmoc label is behaving as an ionization tag for the entire peptide, lowering the ionization potential of the complex below the 7.87-eV photon energy. This method should allow detection of many molecular species covalently or electrostatically bound to surfaces.     

Single-molecule fluorescence trajectories for investigating molecular transport in thin silica sol-gel films

Single-molecule fluorescence tracking has been used to examine diffusion of small molecules in sol-gel films in order to identify spatial heterogeneity in the structure and molecular diffusivities for different regions of the film. Fluorescence intensity profiles from single molecules are fit to a two-dimensional Gaussian function to determine their x,y positions with subpixel resolution. Scatter plots and histograms of molecular step sizes indicate that the trajectories conform to the predictions of a two-dimensional random walk. The mean-square step size is shown to be an unbiased estimate of the variance of the step-size probability distribution and a valid statistic for determining the diffusion coefficient from a molecular trajectory. The diffusion coefficients measured for different molecules are subjected to an F test, which showed that the sol-gel film exhibits spatial variation in the diffusion coefficient on a micrometer-length scale. The spatial variation in diffusivities is a measure of structural heterogeneity of these films.     

Quality of life in advanced prostate cancer: results of a randomized therapeutic trial

BACKGROUND: For patients with metastatic prostate cancer, treatment is primarily palliative, relying mainly on the suppression of systemic androgen hormone levels. To help document the achievement of palliation and to characterize positive and negative effects of treatment, we evaluated quality-of-life (QOL) parameters in patients with metastatic prostate cancer who were randomly assigned to two methods of androgen deprivation. METHODS: Patients (n = 739) with stage M1 (bone or soft tissue metastasis) prostate cancer were enrolled in a QOL protocol that was a companion to Southwest Oncology Group INT-0105, a randomized double-blind trial comparing treatment with bilateral orchiectomy (surgical castration) plus either flutamide or placebo. Patients completed a comprehensive battery of QOL questionnaires at random assignment to treatment and at 1, 3, and 6 months later. Data were collected on three treatment-specific symptoms (diarrhea, gas pain, and body image), on physical functioning, and on emotional functioning. All P values are two-sided. RESULTS: Questionnaire return rates for this study never dropped below 80%; only 2% of the patients did not submit baseline QOL assessments. Cross-sectional analyses (corrected for multiple testing) identified statistically significant differences that favored orchiectomy plus placebo for two of the five primary QOL parameters as follows: patients receiving flutamide reported more diarrhea at 3 months (P = .001) and worse emotional functioning at 3 and 6 months (both P<.003). Longitudinal analyses replicated these findings. Other analyzed QOL parameters favored the group receiving placebo but were not statistically significant after adjustment for multiple testing. CONCLUSIONS: We found a consistent pattern of better QOL outcomes at each follow-up assessment during the first 6 months of treatment for orchiectomized patients with metastatic prostate cancer who received placebo versus flutamide. Improvement over time was evident in both treatment groups but more so for patients receiving placebo.     

False categories in cognition: the Not-the-Liver fallacy

This paper reports on an increasingly frequent error committed in cognition research that at best slows progress, and at worse leads to self-perpetuating false claims and misguided research. The error involves how we identify meaningful processes and categories on the basis of data. Examples are given from three areas of cognition: (1) memory, where the misconception has fueled the popular implicit/explicit categories, (2) perception, where the misconception is used to re-evaluate the classic what/where division, and (3) motor skills, where it is used to draw conclusions from patients with Huntington's disease. Reasons for the prevalence of this error, how it relates to double dissociations, and what it suggests about scientific reasoning are offered.     

The effects of incremental submaximal exercise on circulating leukocytes in physically active and sedentary males and females

The presence of HIV-1 DNA sequence variants of pediatric AIDS patients was investigated in a two-stage polymerase chain reaction (PCR) using nested primers for the first and second (V1-V2) hypervariable regions of the proviral envelope (gp 120) gene (env1). Gel electrophoresis analysis yielded amplified DNA bands which exhibited length variations which were characteristic for each child. The PCR products were cloned and the resulting clones demonstrated inserts of different lengths in a patient and between patients. Analysis of five clones from two different patients at the level of DNA sequencing indicates an extreme heterogeneity in the V1-V2 region. DNA maximum similarity between all of the isolated clones ranged between 69 to 96%. Comparison between DNA sequences of the isolated clones and HIV-1 strains of other parts of the world was also performed. The highest percentage of similarity that was found with known HIV variants included the following strains: HIVADA, HIVJFL, HIVSW42, HIVSWB83, HIVTRA2, HIVWMJ22. The sequences also showed a high degree of similarity to the clade B virus HIVSF162. The analyzed HIV-1 sequences demonstrated the expected high degree of variation in the V1-V2 region of the envelope gene and in some cases that the variation between isolates from the same patient may exceed the variation between the individual clones and the reference HIV-1 strains.