Characteristics and capabilities of the hyperspectral imaging microscope

Single-pass multispectral imaging enables applications ranging from whole-chromosome painting to gene mapping. We have developed a prototype Hyperspectral Imaging Microscope (HIM) Workstation to take advantage of multispectral imaging analysis on a single pass. We trade a full specimen image on a single pass for a full emission spectrum on a single pass. The prototype HI microscope collects the visible emission spectrum from a microscope slide by multiple exposures of the CCD camera as it steps across the slide. On each step, one dimension is space and the other is wavelength. The software correlates spatial and spectral information with minimal or no use of optical filters, offering important advantages over a standard epi-fluorescence microscope.     

Thermo-Mixed Hydrodynamics of Piston Compression Ring Conjunction

A new method, comprising Navier–Stokes equations, Rayleigh–Plesset volume fraction equation, an analytical control-volume thermal-mixed approach and asperity interactions, is reported. The method is employed for prediction of lubricant flow and assessment of friction in the compression ring–cylinder liner conjunction. The results are compared with Reynolds-based laminar flow with Elrod cavitation algorithm. Good conformance is observed for medium load intensity part of the engine cycle. At lighter loads and higher sliding velocity, the new method shows more complex fluid flow, possessing layered flow characteristics on the account of pressure and temperature gradient into the depth of the lubricant film, which leads to a cavitation region with vapour content at varied volume fractions. Predictions also conform well to experimental measurements reported by other authors.