Timing HIV infection with a simple and accurate population viral dynamics model

Clinical trials for HIV prevention can require knowledge of infection times to subsequently determine protective drug levels. Yet, infection timing is difficult when study visits are sparse. Using population nonlinear mixed-effects (pNLME) statistical inference and viral loads from 46 RV217 study participants, we developed a relatively simple HIV primary infection model that achieved an excellent fit to all data. We also discovered that Aptima assay values from the study strongly correlated with viral loads, enabling imputation of very early viral loads for 28/46 participants. Estimated times between infecting exposures and first positives were generally longer than prior estimates (average of two weeks) and were robust to missing viral upslope data. On simulated data, we found that tighter sampling before diagnosis improved estimation more than tighter sampling after diagnosis. Sampling weekly before and monthly after diagnosis was a pragmatic design for good timing accuracy. Our pNLME timing approach is widely applicable to other infections with existing mathematical models. The present model could be used to simulate future HIV trials and may help estimate protective thresholds from the recently completed antibody-mediated prevention trials.     

Rectal trauma: management based on anatomic distinctions

Principles of rectal wound management, including routine diversion, injury repair, presacral drainage and distal washout, evolved from World War II and the Vietnam conflict and have been questioned in recent years. We believe significant confusion arises because of imprecise definition of injury location relative to retroperitoneal involvement. Our 5-year experience with penetrating rectal injuries at a Level I trauma center was analyzed. Injuries to the anterior and lateral surfaces of the upper two-thirds of the rectum were classified as intraperitoneal (IP, serosalized), and those of the posterior surface extraperitoneal (EP, no serosa); injuries to the lower one-third were EP. A total of 58 injuries were managed (92% gunshot wounds). Of these, 16 were IP, and 42 had some EP component. Ten patients underwent repair without diversion (6 IP, 4 EP); there were no leaks. Ten septic complications occurred in the remaining population: 2 necrotizing fasciitis, 5 abdominal abscess, and 3 presacral infections (PIs) (2 presacral abscesses and 1 wound tract infection). PI is the only complication that can be specifically associated with EP rectal injuries relative to management; as associated injury confounds interpretation of the other complications. The operative management in the 38 patients with diverted EP wounds with respect to presacral infection (PI) demonstrated the following: repair injury (n = 10), 0 PI versus no repair (n = 28), 3 PI (P = 0.55); washout (n = 33), 2 PI versus no washout (n = 5), 1 PI (P = 0.35); presacral drain (n = 30), 1 PI versus no drain (n = 8), 2 PI (P = 0.11). We conclude that most IP injuries can be managed with primary repair. EP wounds to the upper two-thirds of the rectum should usually be repaired. EP wounds to the lower one-third, which are explored and repaired, do not require drainage. EP wounds that are not explored should be managed with presacral drainage to minimize the incidence of presacral abscess.     

Prophylactic Greenfield filters: acute complications and long-term follow-up

The efficacy of prophylactic vena caval filters (VCF) in reducing morbidity and mortality from pulmonary embolism (PE) in high-risk trauma patients has been shown, but minimal follow-up data is currently available. VCFs were prophylactically placed in 110 patients between August 1991 and June 1995. There was an early VCF complication rate of 7%. Twenty-two patients died; the remaining 88 patients formed the basis for the follow-up study. Forty-five patients were located and interviewed by phone, and 30 of these patients (34%) returned for evaluation. The mean follow-up time was 18 months (range, 4-42 months). There was no incidence of caval thrombosis on follow-up. Eleven patients had physical findings, and duplex evidence consistent with postphlebitic syndrome. An additional three patients had evidence of old deep venous thrombosis (DVT) by duplex, but no significant symptomatology. VCF are effective in preventing PE related deaths and have few major complications. The long-term morbidity associated with posttraumatic venous thrombosis is significant. This morbidity is related not to PE or VCF, but to the underlying DVT. Improved strategies against DVT are necessary.     

Connectivity Based k-Hop Clustering in Wireless Networks

In this paper we describe several new clustering algorithms for nodes in a mobile ad hoc network. The main contribution is to generalize the cluster definition and formation algorithm so that a cluster contains all nodes that are at distance at most k hops from the clusterhead. We also describe algorithms for modifying cluster structure in the presence of topological changes. We also proposed an unified framework for most existing and new clustering algorithm where a properly defined weight at each node is the only difference in otherwise the same algorithm. This paper studied node connectivity and node ID as two particular weights, for k=1 and k=2. Finally, we propose a framework for generating random unit graphs with obstacles.     

Some aspects of the biology of Dermestes maculatus Degeer (Coleoptera,Dermestidae) in dried fish

The development of Dermestes maculatus Deg. in dried fish has been studied under uncontrolled laboratory conditions. Females laid eggs within 12 hr of copulation, and oviposition was improved by the presence of free water. Hatching occurred about 48 hr after oviposition. The characteristic appearance of the egg and the changes that occurred during incubation and hatching are described. Larval development was completed in 33·5 days during which seven moults occurred and a body length of 14 mm was attained. Larval life was prolonged by crowding. When intact pieces of fish were available, the last instar larva bored into one of them and pupated within the hardened larval skin, but when ground fish was provided, a quiescent prepupal stage was observed. The adult emerged about 11 days after the last instar larval stage, irrespective of the mode of pupation.     

Conduction in thin polymer films

Thin polymer films have been made from a variety of starting monomers and their electrical conduction has been studied.The films are produced both by ultraviolet surface photolysis and by a cold-cathode glow-discharge technique. The samples are made in the form of a capacitor sandwich on a glass substrate with aluminium electrodes. The whole sample is made in a high-vacuum system, without breaking vacuum, using out-of-contact masking techniques.Generally, depending on the starting monomer, the polymer films are short-free down to a thickness of about 100 Å, and electric fields up to 10⁷ V/cm may be sustained.The current/voltage characteristics of the films have been investigated as a function of temperature and thickness. One theory to account for the conduction in these materials is presented and compared with other approaches to the problem.     

Fast multiplication in VLSI using wave pipelining techniques

Wave pipelining is a design methodology that can increase the clock frequency of digital systems. Also known asmaximum-rate pipelining, it has long been considered a technique for approaching the physical speed limit of a digital circuit. Unlike conventional pipelining, wave pipelining does not require internal clocked elements to increase throughput. The synchronization of internal computations is achieved by balancing inherent RC delays of combinational logic elements, thus allowing circuits to be pipelined at a very fine-grain level. In this article, we describe the design of a 16×16 wave-pipelined multiplier using a 1.0 μm CMOS process. The multiplier is designed using a conventional static CMOS technology. Simulation results show a speedup of about 7× over a nonpipeline implementation.     

Organic Phenolic Configurationally Locked Polyene Single Crystals for Electro‐optic and Terahertz Wave Applications

We investigate a configurationally locked polyene (CLP) crystal 2‐(3‐(4‐hydroxystyryl)‐5,5‐dimethylcyclohex‐2‐enylidene)malononitrile (OH1) containing a phenolic electron donor, which also acts as a hydrogen bond donor. The OH1 crystals with orthorhombic space group Pna2₁ (point group mm2) exhibit large second‐order nonlinear optical figures of merit, high thermal stability and very favorable crystal growth characteristics. Higher solubility in methanol and a larger temperature difference between the melting temperature and the decomposition temperature of OH1 compared to analogous CLP crystals, are of advantage for solution and melt crystal growth, respectively. Acentric bulk OH1 crystals of large sizes with side lengths of up to 1 cm with excellent optical quality have been successfully grown from methanol solution. The microscopic and macroscopic nonlinearities of the OH1 crystals are investigated theoretically and experimentally. The OH1 crystals exhibit a large macroscopic nonlinearity with four times larger powder second harmonic generation efficiency than that of analogous CLP crystals containing dimethylamino electron donor. A very high potential of OH1 crystals for broadband THz wave emitters in the full frequency range of 0.1–3 THz by optical rectification of 160 fs pulses has been demonstrated.     

Bioinspired Design of Lysolytic Triterpenoid–Peptide Conjugates that Kill African Trypanosomes

Humans have evolved a natural immunity against Trypanosoma brucei infections, which is executed by two serum (lipo)protein complexes known as trypanolytic factors (TLF). The active TLF ingredient is the primate-specific apolipoprotein L1 (APOL1). The protein has a pore-forming activity that kills parasites by lysosomal and mitochondrial membrane fenestration. Of the many trypanosome subspecies, only two are able to counteract the activity of APOL1; this illustrates its evolutionarily optimized design and trypanocidal potency. Herein, we ask whether a synthetic (syn) TLF can be synthesized by using the design principles of the natural TLF complexes but with different chemical building blocks. We demonstrate the stepwise development of triterpenoid–peptide conjugates, in which the triterpenoids act as a cell-binding, uptake and lysosomal-transport modules and the synthetic peptide GALA acts as a pH-sensitive, pore-forming lysolytic toxin. As designed, the conjugate kills infective-stage African trypanosomes through lysosomal lysis thus demonstrating a proof-of-principle for the bioinspired, forward-design of a synTLF.     

Advancements in the Field of Direct Steam Generation in Linear Solar Concentrators—A Review

Direct steam generation in parabolic trough or linear Fresnel collectors represents one interesting technological option for concentrating solar electricity production. Today's state of the art characterized by the first commercial plants in operation is a result of more than 20 years of intensive research on this topic. This article provides a review on the key results from research that includes physical effects like heat transfer and pressure drop in horizontal boiler tubes, plant layout considerations, and thermal storage options. An overview on test and demonstration facilities as well as on commercial plants is given, leading to an outlook on the next generation of direct steam generation systems.