全文获取类型
收费全文 | 654786篇 |
免费 | 9055篇 |
国内免费 | 2095篇 |
专业分类
电工技术 | 11867篇 |
综合类 | 1086篇 |
化学工业 | 98227篇 |
金属工艺 | 25541篇 |
机械仪表 | 20372篇 |
建筑科学 | 14776篇 |
矿业工程 | 3958篇 |
能源动力 | 17081篇 |
轻工业 | 50796篇 |
水利工程 | 7137篇 |
石油天然气 | 12901篇 |
武器工业 | 32篇 |
无线电 | 73893篇 |
一般工业技术 | 127609篇 |
冶金工业 | 130366篇 |
原子能技术 | 14371篇 |
自动化技术 | 55923篇 |
出版年
2021年 | 5434篇 |
2020年 | 4132篇 |
2019年 | 5222篇 |
2018年 | 9372篇 |
2017年 | 9488篇 |
2016年 | 10548篇 |
2015年 | 6806篇 |
2014年 | 10464篇 |
2013年 | 29397篇 |
2012年 | 17979篇 |
2011年 | 24070篇 |
2010年 | 18182篇 |
2009年 | 20707篇 |
2008年 | 21064篇 |
2007年 | 20737篇 |
2006年 | 18500篇 |
2005年 | 19371篇 |
2004年 | 17787篇 |
2003年 | 17169篇 |
2002年 | 15513篇 |
2001年 | 15231篇 |
2000年 | 13966篇 |
1999年 | 14595篇 |
1998年 | 39610篇 |
1997年 | 27753篇 |
1996年 | 21198篇 |
1995年 | 15583篇 |
1994年 | 13740篇 |
1993年 | 13655篇 |
1992年 | 9675篇 |
1991年 | 9268篇 |
1990年 | 9181篇 |
1989年 | 8881篇 |
1988年 | 8617篇 |
1987年 | 7359篇 |
1986年 | 7300篇 |
1985年 | 8250篇 |
1984年 | 7617篇 |
1983年 | 6954篇 |
1982年 | 6533篇 |
1981年 | 6713篇 |
1980年 | 6314篇 |
1979年 | 6069篇 |
1978年 | 6225篇 |
1977年 | 7497篇 |
1976年 | 10102篇 |
1975年 | 5468篇 |
1974年 | 5097篇 |
1973年 | 5236篇 |
1972年 | 4453篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
K Miwa M Asano R Horai Y Iwakura S Nagata T Suda 《Canadian Metallurgical Quarterly》1998,4(11):1287-1292
Anchietia salutaris tea is traditionally used in Brazil to treat allergies, suggesting it contains compounds with antagonistic activity on the allergic mediators. We have evaluated extracts and semi-purified fractions of Anchietia salutaris as a source of compounds having this type of antagonism on the contraction induced in guinea-pig lung parenchymal strips and on platelet aggregation and shape change. After 10 min pre-incubation dichloromethane extracts containing 30 or 100 microg mL(-1) inhibited the contraction induced by prostaglandin D2 (PGD2) in guinea-pig lung parenchymal strips with dose ratios (DR) of 0.76+/-0.14 and 0.93+/-0.19, respectively; the amount of inhibition depended both on the concentration and on the time of pre-incubation (DR after 30 min pre-incubation was 1.21+/-0.51). The dichloromethane extract and its semi-purified fractions also inhibited the contractions induced by U46619, a more potent, stable, synthetic agonist of thromboxane A2 (TxA2) prostanoid (TP) receptors, the receptors acted upon by PGD2 to produce lung contractions. The dichloromethane extract did not inhibit the lung parenchymal contractions induced by histamine, leukotriene D4 (LTD4) or platelet-activating factor (PAF). Platelet aggregation induced by U46619, adenosine 5'-diphosphate (ADP) or PAF was not inhibited by the dichloromethane extract. Indeed, the extract potentiated platelet aggregation induced by low concentrations of these agonists and also potentiated the shape change induced by U46619. These results imply that the dichloromethane extract of Anchietia salutaris and its semi-purified fractions contain an active principle that competitively inhibits TxA2 TP receptors, the stimulation of which causes lung parenchymal contraction. The inhibition seems to be selective for this receptor subtype, because the extract fails to inhibit platelet aggregation or shape change. This provides additional support of earlier reports suggesting the occurrence of TP receptor subtypes. 相似文献
992.
M Sternfeld G Ming H Song K Sela R Timberg M Poo H Soreq 《Canadian Metallurgical Quarterly》1998,18(4):1240-1249
Accumulated indirect evidence suggests nerve growth-promoting activities for acetylcholinesterase (AChE). To determine unequivocally whether such activities exist, whether they are related to the capacities of this enzyme to hydrolyze acetylcholine and enhance synapse development, and whether they are associated with alternative splicing variants of AChEmRNA, we used four recombinant human AChEDNA vectors. When Xenopus laevis embryos were injected with a vector expressing the synapse-characteristic human AChE-E6, which contains the exon 6-encoded C terminus, cultured spinal neurons expressing this enzyme grew threefold faster than co-cultured control neurons. Similar enhancement occurred in neurons expressing an insertion-inactivated human AChE-E6-IN protein, containing the same C terminus, and displaying indistinguishable immunochemical and electrophoretic migration properties from AChE-E6, but incapable of hydrolyzing acetylcholine. In contrast, the nonsynaptic secretory human AChE-I4, which contains the pseudointron 4-derived C terminus, did not affect neurite growth. Moreover, no growth promotion occurred in neurons expressing the catalytically active C-terminally truncated human AChE-E4, demonstrating a dominant role for the E6-derived C terminus in neurite extension. Also, AChE-E6 was the only active enzyme variant to be associated with Xenopus membranes. However, postsynaptic length measurements demonstrated that both AChE-E6 and AChE-E4 enhanced the development of neuromuscular junctions in vivo, unlike the catalytically inert AChE-E6-IN and the nonsynaptic AChE-I4. These findings demonstrate an evolutionarily conserved synaptogenic activity for AChE that depends on its hydrolytic capacity but not on its membrane association. Moreover, this synaptogenic effect differs from the growth-promoting activity of AChE, which is unrelated to its hydrolytic capacity yet depends on its exon 6-mediated membrane association. 相似文献
993.
994.
H Sawert E Girardi G Antonucci MC Raviglione P Viale G Ippolito 《Canadian Metallurgical Quarterly》1998,158(19):2112-2121
995.
996.
Robben J.; der Schueren J.Van; Verhasselt P.; Aert R.; Volckaert G. 《Protein engineering, design & selection : PEDS》1995,8(2):159-165
The deletion of nine residues from the C-terminus of the bacterialchloramphenicol acetyltransferase (CAT) results in depositionof the mutant protein in cytoplasmic inclusion bodies and lossof chloramphenicol resistance in Escherichia coli. This foldingdefect is relieved by C-terminal fusion of the polypeptide withas few as two residues. Based on these observations, efficientpositive selection for the cloning of DNA fragments has beendemonstrated. The cloning vector encodes a C-terminally truncatedCAT protein. Restriction sites in front of the stop codon allowthe insertion of target DNA, resulting in the production ofproperly folded CAT fusion proteins and regained chloramphenicolresistance. The positive selection of recombinants is accomplishedby growth of transformants on chloramphenicol-containing agarplates. The method appears particularly convenient for the cloningof DNA fragments amplified by the PCR because minimal informationto restore CAT folding can be included in the primers. The cloningof random sequences shows that the folding defect can be relievedby fusion to a wide variety of peptides, providing great flexibilityto the positive selection system. This vector may also contributeto the determination of the role of the C-terminus in CAT folding. 相似文献
997.
Studies static-state feedback control of discrete-event systems under partial observation. The authors present a necessary and sufficient condition for the existence of a static-state feedback controller. A static controller is a special case of a dynamic controller studied by Kumar et al. The authors' condition, however, has computational advantage in contrast to those obtained by Kumar et al. The authors also present a necessary and sufficient condition under which the modular feedback synthesis is possible 相似文献
998.
E Bazopoulou-Kyrkanidou P Neou CS Bartsocas S Kyrkanides I Fanourakis 《Canadian Metallurgical Quarterly》1995,6(3):227-232
PURPOSE: To select ideal radiologic projections for mapping and ablation of tachycardias of right ventricular outflow tract (RVOT). METHODS: Ten hearts from human corpses were studied utilizing radiopaque material to identify the pulmonary valve and three distinct sites on this valve: septal anterior (A), septal posterior (P) and free-wall (L). Next, the hearts were filmed in the frontal plane and in oblique projections with 15 degrees increments to the right and to the left. The projections in which the sites were lateralized on the valve, eased radiologic interpretation and were considered ideal for mapping and ablation. Depending on the proximity of the sites to the lateral extremes of the pulmonary valve, the projections were considered ideal ( ), intermediary (++) and inadequate (+). RESULTS: Projections [table: see text] CONCLUSION: The A site of RVOT was best indicated in the 60 and 45 degrees left anterior oblique projections; the 0 degree postero anterior projection was best for mapping the P site; the L region was best explored in the 60 degrees right anterior oblique projection. 相似文献
999.
1000.
Several in vivo and in vitro methods for monitoring immunological properties of two allergoids obtained by formaldehyde treatment of ovalbumin (OA) were developed. The calculated molecular weight of allergoids was 80 kD (OA-F1) and 165 kD (OA-F2), respectively. The allergenic activity in vitro of allergoids in mast-cell histamine release assay was 1000 times lower than of OA. Both allergoids showed reduced ability to induce passive cutaneous anaphylaxis in the Sprague-Dawley rats or systemic anaphylaxis in Dunkin-Harley guinea-pigs. The ability of OA and allergoids to bind to the OA-specific IgE antibodies was measured in vivo by the inhibition of passive cutaneous anaphylaxis (PCA-inhibition). Allergoid binding to IgE was 51-66% lower than the native allergen. Moreover, the avidity of OA-specific IgG antibodies, measured by ELISA-inhibition, for allergoids and allergen was of the same order. Allergoids induced a different pattern of humoral immune response from that, induced by the native allergen. Thus, after immunization of BALB/c mouse, both allergoids induced a higher production of IgG and a lower production of IgE than OA, only OA-F2 induced a lower production of IgG1. The differences in the IgA response to the immunogens was not significant. Delayed hypersensitivity studies in the BALB/c mouse showed that allergoids were 5- to 12-times less effective in inducing a cell-mediated immune response than OA. The present study provides a battery of immunological methods for preclinical testing of modified allergens. 相似文献