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261.
JM Shin M Kajimura JM Argüello JH Kaplan G Sachs 《Canadian Metallurgical Quarterly》1994,269(36):22533-22537
The transmembrane segments of sarcoplasmic reticulum Ca(2+)-ATPase were determined by trypsinization of cytoplasmic side-out intact sarcoplasmic reticulum vesicles. The membrane portion of tryptic digest comprising the transmembrane fragments, joined by the intravesicular segments, was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after labeling with fluorescein 5-maleimide in the presence of sodium dodecyl sulfate. In this way, seven fluorescent bands of tryptic fragments below 11 kDa were observed which were derived from 4 pairs of membrane spanning segments and one hydrophobic sequence at the C-terminal end. Two peptides of 10.8 and 10.6 kDa had the identical N-terminal sequence beginning at Glu826, representing the transmembrane segments M7 and M8 and their connecting loop. A band at 8.1 kDa contained one peptide beginning at Tyr36 (M1/loop/M2). A 7.7-kDa peptide starting at Leu253 (M3/loop/M4) and a 7.3-kDa peptide beginning at Ala752 (M5/loop/M6) were also observed. A band at 6.7 kDa contained two peptides, one beginning at Ser48 (M1/loop/M2) and another beginning at Tyr763 (M5/loop/M6). In addition, a 4-kDa peptide beginning at Met925 was observed. The size of this peptide did not allow for a complete pair of transmembrane segments, but this peptide could have been derived from trypsinolysis between the last pair of membrane spanning segments. These data therefore provide biochemical evidence for at least 8 transmembrane segments and perhaps two more at the C-terminal end of the enzyme. 相似文献
262.
A Gopal G Li Mandri DL King C Marboe S Homma 《Canadian Metallurgical Quarterly》1994,105(6):1885-1887
Cardiac papillary fibroelastomas are unusual, frond-like growths typically found on cardiac valves, diagnosed incidentally on autopsy or cardiac surgery, but rarely during life. We report a rare case of an aortic valve papillary fibroelastoma detected by transthoracic echocardiography and confirmed by histologic study. 相似文献
263.
Effect of Thermohydrogen Treatment on the Structure and Properties of Titanium Alloy Castings 总被引:2,自引:0,他引:2
Il'in A. A. Skvortsova S. V. Mamonov A. M. Permyakova G. V. Kurnikov D. A. 《Metal Science and Heat Treatment》2002,44(5-6):185-189
Metal Science and Heat Treatment - 相似文献
264.
Corazza G.E. Ferrari G. 《IEEE transactions on information theory / Professional Technical Group on Information Theory》2002,48(11):3003-3008
New bounds are proposed for the Marcum Q-function, which is defined by an integral expression where the 0th-order modified Bessel function appears. The proposed bounds are derived by suitable approximations of the 0th-order modified Bessel function in the integration region of the Marcum Q-function. They prove to be very tight and outperform bounds previously proposed in the literature. In particular, the proposed bounds are noticeably good for large values of the parameters of the Marcum Q-function, where previously introduced bounds fail and where exact computation of the function becomes critical due to numerical problems 相似文献
265.
G.D. Wignall R.W. Hendricks W.C. Koehler J.S. Lin M.P. Wai E.L. Thomas R.S. Stein 《Polymer》1981,22(7):886-889
A series of small angle neutron scattering measurements on blends of normal polystyrene (PSH) and labelled (deuterated) polystyrene (PSD) have been made with concentrations of PSD from 5 to 50 mol %. It is shown that the single chain form factor of the polymer in bulk can be obtained from a single concentration measurement for any concentration of labelled molecules, providing the molecular weights of the parent and labelled molecules are the same and the molecular weight distributions are narrow. 相似文献
266.
267.
Compensated SC integrator stages are analysed and compared with respect to their sensitivities to finite amplifier gain effects. Simple single-stage amplifiers may be used in such circuits even in highly selective filtering applications. This may allow an extension of the present frequency range of SC circuits. 相似文献
268.
The survey focuses on algebraic-grammatical models of parallel processes, representation of knowledge about classes of algorithms in terms of a variety of production systems (structured design grammars), and program generation tools.Translated from Kibernetika i Sistemnyi Analiz, No. 5, pp. 5–13, September–October, 1991. 相似文献
269.
270.