Apoptosis in the failing human heart

BACKGROUND: Loss of myocytes is an important mechanism in the development of cardiac failure of either ischemic or nonischemic origin. However, whether programmed cell death (apoptosis) is implicated in the terminal stages of heart failure is not known. We therefore studied the magnitude of myocyte apoptosis in patients with intractable congestive heart failure. METHODS: Myocardial samples were obtained from the hearts of 36 patients who underwent cardiac transplantation and from the hearts of 3 patients who died soon after myocardial infarction. Samples from 11 normal hearts were used as controls. Apoptosis was evaluated histochemically, biochemically, and by a combination of histochemical analysis and confocal microscopy. The expression of two proto-oncogenes that influence apoptosis, BCL2 and BAX, was also determined. RESULTS: Heart failure was characterized morphologically by a 232-fold increase in myocyte apoptosis and biochemically by DNA laddering (an indicator of apoptosis). The histochemical demonstration of DNA-strand breaks in myocyte nuclei was coupled with the documentation of chromatin condensation and fragmentation by confocal microscopy. All these findings reflect apoptosis of myocytes. The percentage of myocytes labeled with BCL2 (which protects cells against apoptosis) was 1.8 times as high in the hearts of patients with cardiac failure as in the normal hearts, whereas labeling with BAX (which promotes apoptosis) remained constant. The near doubling of the expression of BCL2 in the cardiac tissue of patients with heart failure was confirmed by Western blotting. CONCLUSIONS: Programmed death of myocytes occurs in the decompensated human heart in spite of the enhanced expression of BCL2; this phenomenon may contribute to the progression of cardiac dysfunction.     

Analogue micropowered log-domain tone controller for auditoryprostheses

A micropower tone-control circuit comprising two first-order log-domain filters and a subtractor built with MOS transistors in weak inversion is described. The tone-controller is capable of providing bass cut/boost and treble cut operations. This circuit is being used in new designs of a conventional (acoustic) hearing-aid and cochlear implant     

Alcohol in Munich secondary school students--a study for dissemination of experiences, knowledge, attitude and behavior

The Munich Community Health Service (Medical School-Service Department) conducted an alcohol prevention project in ten public secondary schools. The 423 pupils (11-17 years of age) filled in a questionnaire on alcohol and discussed it afterwards under the guidance of a school physician and a teacher using guideline information. The results of the survey are presented in this paper. Additionally, suggestions for transferring the results to conceptualize and carry out preventive measures are made. The results indicate that the first experience with alcohol occurred early in life. One-fifth of the sample had already tried alcohol at the age of six. Immediate effects of alcohol (e.g. state of drunkenness) were experienced by 35.7 percent of the sample group. Kiosks, shops, and beverage stores were named most frequently as sources for alcohol. The sources of social support varied with respect to sex and age. With increasing age of the children, parents were contacted less frequently whereas the role of peers increased. The present results supply possible paths of action for the development of successful prevention strategies, e.g. by pointing out the early age of onset as well as specific sources of alcohol acquisition.     

Polymerase chain reaction versus culture for detection of Ureaplasma urealyticum and Mycoplasma hominis in the urogenital tract of adults and the respiratory tract of newborns

The efficiency of the polymerase chain reaction (PCR) was compared with that of culture for detection of Ureaplasma urealyticum and Mycoplasma hominis in 726 clinical specimens comprising 189 gynecological samples, 362 urological samples, and 175 samples from newborn infants. The sensitivity of PCR versus culture was 95% for both organisms, while the sensitivity of culture versus PCR was 91% for Ureaplasma urealyticum and 84% for Mycoplasma hominis. Furthermore, PCR tests were faster than culture tests, allowing the time to diagnosis to be reduced from two to five days to 24 h.     

Extended-window interpolation applied to digital filter design

This paper develops a procedure for the design of frequency-selective interpolation operators that can be computed and saved once and for all. These operators are used to design real-time digital operators: interpolators, FIR differentiators, IIR filters, and composed interpolation and filtering operators. Each real-time operator is a matrix relating sets of data points to sets of interpolated values. Since these matrices are characterized by low norms, they permit reduced-word implementations, and are suitable for real-time processing with array processors and massively parallel machines. The design of the interpolation operators uses windows that, unlike traditional approaches, extend beyond the data interval up to the length permitted by the dimensionality theorem. A new form of the dimensionality theorem is used to minimize the minimax interpolation error within a predetermined frequency range, which may be either the passband of the antialiasing filter or the passband of an analog prototype filter. The main application presented in the paper is the design of combined digital filters and interpolators, which will be referred to as interpolating filters. The frequency responses of such filters, as well as the interpolated time responses, almost coincide with those of the corresponding analog prototypes     

In-field comparison among polarization-mode-dispersion measurementtechniques

We report polarization mode dispersion measurements on a 168 km long undersea optical cable, performed with two techniques. From experimental results, statistical behavior of polarization parameters and their time fluctuations have been extracted. An average differential group delay of about 13 ps shows that dispersion-shifted fibers can be affected by a polarization dispersion significantly larger than standard step-index fibers. The correlation length of the polarization-coupling perturbation in the tested fibers is not negligible with respect to the link length     

Electromigration drift and threshold in Cu thin-film interconnects

The electromigration-induced ionic drift velocity and critical length-current density product, (jl_c) of Cu thin film conductors, were measured using the Blech-Kinsbron edge-displacement technique. Unencapsulated Cu edge-displacement segments on TiN conductors were stressed in vacuum at a modest current density of 6×10⁵ A/cm² in the temperature range of 175-275°C. Drift velocity was observed to be between 1-1/2 to 3 orders-of-magnitude lower than that previously measured for unencapsulated Al in this temperature range. We measured an activation energy for EM-induced drift of 1.25±0.08 eV which corresponds to grain boundary diffusion in Cu. Critical lengths were measured and the jl_c threshold was estimated to range between 900-1600 A/cm. We calculated a Cu grain boundary Z* value of -0.7, to our knowledge, this study is the first to measure Z* for electromigration in Cu thin film conductors     

On the measurement of SSB noise figure using sideband cancellation

A new method for determining the single-sideband noise figure of a device from double-sideband measurements is proposed which eliminates the need for tunable filters and hence greatly reduces the complexity and cost of performing accurate noise figure measurements. This paper compares results obtained to those obtained using a traditional single-sideband measurement system with a tuneable filter and provides a full error analysis of the new technique. The method also offers considerable possibilities for future use at higher (microwave) frequencies where YIG filters and image reject mixers are not readily available