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81.
Mumcuoglu EU Hassanpour R Tasel SF Perkins G Martone ME Gurcan MN 《Journal of microscopy》2012,246(3):248-265
Mitochondrial function plays an important role in the regulation of cellular life and death, including disease states. Disturbance in mitochondrial function and distribution can be accompanied by significant morphological alterations. Electron microscopy tomography (EMT) is a powerful technique to study the 3D structure of mitochondria, but the automatic detection and segmentation of mitochondria in EMT volumes has been challenging due to the presence of subcellular structures and imaging artifacts. Therefore, the interpretation, measurement and analysis of mitochondrial distribution and features have been time consuming, and development of specialized software tools is very important for high-throughput analyses needed to expedite the myriad studies on cellular events. Typically, mitochondrial EMT volumes are segmented manually using special software tools. Automatic contour extraction on large images with multiple mitochondria and many other subcellular structures is still an unaddressed problem. The purpose of this work is to develop computer algorithms to detect and segment both fully and partially seen mitochondria on electron microscopy images. The detection method relies on mitochondria's approximately elliptical shape and double membrane boundary. Initial detection results are first refined using active contours. Then, our seed point selection method automatically selects reliable seed points along the contour, and segmentation is finalized by automatically incorporating a live-wire graph search algorithm between these seed points. In our evaluations on four images containing multiple mitochondria, 52 ellipses are detected among which 42 are true and 10 are false detections. After false ellipses are eliminated manually, 14 out of 15 fully seen mitochondria and 4 out of 7 partially seen mitochondria are successfully detected. When compared with the segmentation of a trained reader, 91% Dice similarity coefficient was achieved with an average 4.9 nm boundary error. 相似文献
82.
83.
GA Kaltsas JJ Mukherjee PN Plowman JP Monson AB Grossman GM Besser 《Canadian Metallurgical Quarterly》1998,83(12):4233-4238
Pituitary tumors are mostly benign lesions, although 5-35% are locally invasive. A small number exhibit a more aggressive course, infiltrating dura, bone and sinuses, and are designated highly aggressive. However, the presence of metastases separate from the pituitary in the central nervous system or at a distance is necessary to designate pituitary tumors as carcinomas, i.e. truly malignant. When conventional therapeutic modalities fail, systemic chemotherapy remains the last option. We report seven such patients, three with highly aggressive and four with malignant pituitary tumors (n=4) four women; median age, 32 yr; range, 23-48 yr), who received one or more courses of chemotherapy with lomustine and 5-fluorouracil (median, two courses; range, one to six courses). Three patients with systemic metastatic disease had a shorter survival (median, 5 months; range, 1-14 months) than the one patient with central nervous system metastases alone (10 yr). A patient with an aggressive nonmetastatic prolactinoma who initially responded to chemotherapy died from another nondisease-associated cause. Two patients, one with an aggressive and one with a metastatic tumor, achieved symptomatic improvement with a median duration of 6 months. A hormonal reduction greater than 50% was observed in two of seven patients; only one patient who had an aggressive tumor obtained an objective tumor response. The median survival from the time of initiation of chemotherapy in patients with malignant tumors ranged from 3-65 months. Two patients with malignant tumors developed disease progression while receiving chemotherapy; no patient with extracranial metastases showed a response. Treatment was well tolerated, with minimal individual side-effects. Three patients with no response to initial treatment received different chemotherapeutic regimens with no additional response. All patients with metastatic malignant tumors eventually died. Treatment with cytotoxic chemotherapy is noncurative, and current experience is limited. Until another more specific form of treatment is available, chemotherapy may still be of some value in patients with highly aggressive and malignant pituitary tumors, at least in achieving a temporary remission or delay in progression. The combination of lomustine/5-fluorouracil proved easy to administer with minimal toxicity, although the response rate was only 14%. Until a more specific treatment is found, an optimal chemotherapeutic regimen needs to be established. 相似文献
84.
R. A. Reiners N. E. Bednarcyk J. E. Covey J. C. Harris Yoshio Hirano S. Kawamura D. A. Leo F. A. Kummerow E. G. Perkins 《Journal of the American Oil Chemists' Society》1947,24(12):505A-426
Journal of the American Oil Chemists' Society - 相似文献
85.
New ideas are required if our organizations are going to survive and grow. Without the ability to think differently, firms can quickly stagnate and decline. A key contributor to the success (or otherwise) of the ideation process is the extent to which organizations provide effective control mechanisms, with existing literature drawing tentative links between this and the subject of organizational vision. The aim of this paper is to explore the control of idea generation in SME contexts, examining the interaction between organizational vision and the guidance of ideation in these settings. Through in‐depth qualitative research with a range of SMEs, this study shows that structure in the form of an outlining framework assists in opening a liminal space for ideation. More importantly, this paper provides empirical evidence, which demonstrates that organizational visions provide this framework by acting as a target, assisting the development of relevant ideas. Ideation, within the SME, is argued to be fundamentally subject to, and contingent upon, the direction of the organization. 相似文献
86.
Greg Perkins Ernest du Toit Greg Cochrane Grant Bollaert 《Energy Sources, Part A: Recovery, Utilization, and Environmental Effects》2016,38(24):3639-3646
Underground coal gasification (UCG) is a process which converts deep, un-mineable or difficult to mine coal resources into syngas which can then be converted into valuable end products such as electric power, liquid fuels, synthetic natural gas and chemicals. This paper provides a summary of the UCG operations conducted at the Chinchilla Demonstration Facility in Australia, focusing on gasifiers constructed using directional drilling. A number of the experiences and key lessons learned from operating multiple underground gasifiers over several years at the facility are described. Implications for the implementation in commercial projects using UCG are also discussed. Finally, the potential of UCG as a method for producing syngas from deep coal is discussed and some of the challenges and opportunities are summarized. 相似文献
87.
采用磷酸硼对木材进行改性处理,测定了处理后试材的阻燃性、抗流失性、吸湿性、力学性能.研究结果表明,磷酸硼处理后试材,吸药量达到20.47 kg/m3以上时,垂直燃烧实验可达到到F-0级水平;通过灼烧实验,磷酸硼处理的木粉在500℃灼烧时残留率可达到97.997%;此改性剂具有较好阻燃性能和一定的抗流失性,常压下磷酸硼处理的樟子松试件LRV为50.28%;木材顺纹抗压强度平均下降了 8.67%,无明显变化;木材抗弯曲压强度平均下降了 27.95%.但磷酸硼阻燃剂在高湿条件下有一定的吸湿性,使用性能有待改进,并应注意采取防护措施. 相似文献
88.
Simon Perkins Patrick Marais James Gain Mark Berman 《Autonomous Agents and Multi-Agent Systems》2013,26(3):354-388
Classic shortest path algorithms operate on graphs, which are suitable for problems that can be represented by weighted nodes or edges. Finding a shortest path through a set of weighted regions is more difficult and only approximate solutions tend to scale well. The Field D* algorithm efficiently calculates an approximate, interpolated shortest path through a set of weighted regions and was designed for navigating robots through terrains with varying characteristics. Field D* operates on unit grid or quad-tree data structures, which require high resolutions to accurately model the boundaries of irregular world structures. In this paper, we extend the Field D* cost functions to 2D triangulations and 3D tetrahedral meshes: structures which model polygonal world structures more accurately. Since robots typically have limited resources available for computation and storage, we pay particular attention to computation and storage overheads when detailing our extensions. We begin by providing analytic solutions to the minimum of each cost function for 2D triangles and 3D tetrahedra. Our triangle implementation provides a 50 % improvement in performance over an existing triangle implementation. While our 3D extension to tetrahedra is the first full analytic extension of Field D* to 3D, previous work only provided an approximate minimization for a single cost function on a 3D cube with unit lengths. Each cost function is expressed in terms of a general function whose characteristics can be exploited to reduce the calculations required to find a minimum. These characteristics can also be exploited to cache the majority of cost functions, producing a speedup of up to 28 % in the 3D tetrahedral case. We demonstrate that, in environments composed of non-grid aligned data, Multi-resolution quad-tree Field D* requires an order of magnitude more faces and between 15 and 20 times more node expansions, to produce a path of similar cost to one produced by a triangle implementation of Field D* on a lower resolution triangulation. We provide examples of 3D pathing through models of complex topology, including pathing through anatomical structures extracted from a medical data set. To summarise, this paper details a robust and efficient extension of Field D* pathing to data sets represented by weighted triangles and tetrahedra, and also provides empirical data which demonstrates the reduction in storage and computation costs that accrue when one chooses such a representation over the more commonly used quad-tree and grid-based alternatives. 相似文献
89.
项目名称:拉什大学医学中心业主:拉什大学医学中心建设地点:美国伊利诺伊州芝加哥市设计单位:帕金斯威尔建筑设计事务所建筑面积:83万平方英尺(约7.7万m2)建筑层数:11层病床数量:386床项目负责人:James Zajac-Market Sector Leader(Principal-in- 相似文献
90.