Myosin isoforms in skeletal muscle in patients with chronic heart decompensation: distribution and correlation with with exercise tolerance

Chronic heart failure (CHF) is accompanied by a reduced exercise capacity, and the symptoms can be at least in part explained by qualitative and quantitative changes in the skeletal muscle composition and metabolism. We have correlated the myosin heavy chain (MHC) composition of the gastrocnemius in 20 patients with different degrees of CHF to expiratory gases measured during maximal cardiopulmonary exercise testing, NYHA functional class and echocardiographic parameters. MHC composition was determined electrophoretically in skeletal muscle needle microbiopsies and the percent distribution calculated by laser densitometry. There was no correlation between ejection fraction, left ventricular end-diastolic and end-systolic diameters and MHC composition. The percentage of MHC 1 (slow aerobic isoform) was positively correlated with peak VO2 (r2 = 0.5, p = 0.0004), ventilatory threshold (VT, r2 = 0.33, p = 0.008), and O2 pulse (peak VO2/HR, r2 = 0.40, p = 0.003). There was a negative correlation between MHC 2a and 2b (fast isoforms) and peak VO2 (r2 = 0.38 and 0.37, p = 0.004, respectively), VT (r2 = 0.2, p = 0.05; r2 = 0.34, p = 0.007, respectively) and O2 pulse (r2 = 0.39, p = 0.003; r2 = 0.23, p = 0.03, respectively). NYHA functional class was also negatively correlated with the same parameters (r2 = 0.2, p = 0.01; r2 = 0.4, p = 0.001; r2 = 0.34, p = 0.006, respectively) as well as with MHC 1 (r2 = 0.62, p = 0.0001). A positive correlation was found between NYHA functional class and MHC 2a and 2b (r2 = 0.46, p = 0.001; r2 = 0.41, p = 0.002, respectively). The severity of heart failure is paralleled by a shift of the MHC pattern toward the fast MHC 2b. The correlation between the magnitude of the MHCs shift, from the slow aerobic to the fast type, with both clinical parameters (NYHA functional class) and functional measurements (peak VO2, VT, O2 pulse) of exercise capacity seem to suggest that changes in skeletal muscle composition may play a key role in exercise tolerance in patients with CHF.     

The Stuttgart positron beam, its performance and recent experiments

A monoenergetic MeV positron (e⁺) beam, with a flux at present of 6 × 10⁴ e⁺/s in the energy range of 0.5 to 6.5 MeV, has been installed at the Stuttgart Pelletron accelerator. The stabilization and the absolute calibration of the energy E is monitored by a Ge detector with real-time feedback; a relative energy stability of ΔE/E 10⁻⁴ is obtained. So far, e⁺e⁻ scattering and annihilation-in-flight experiments for investigating the low-energy e⁺e⁻ interaction as well as β⁺ γ positron lifetime measurements in condensed matter have been performed. The advantages of the β⁺ γ method compared to the conventional γγ coincidence technique have been demonstrated. Recently, triple-coincidence positron “age-momentum correlation” measurements have been carried out on fused quartz. A brief account is given on the development of a “positron clock” aiming at a substantial improvement of the time resolution of the β⁺ γ positron lifetime measurements.     

Biodistribution of radiolabeled monoclonal antibody E48 IgG and F(ab'')2 in patients with head and neck cancer

The amphibian pronephros is fated to die during early development. Pronephric cells undergo apoptosis and their function is replaced by the mesonephros, which becomes the functional kidney of the adult frog. Tadpoles of the northern leopard frog, Rana pipiens, were inoculated with a Lucké tumour herpesvirus (LTV) preparation. Most of the animals developed typical Lucké renal carcinomas at metamorphosis. Fewer developed carcinomas of the pronephric cell type. A pronephric carcinoma, rescued from apoptosis by the herpesvirus, was harvested from a post-metamorphic frog. The tumour was judged to be pronephric by its anatomical location (in the anterior part of the body) and because both mesonephric kidneys were intact and tumour-free upon removal of the tumour mass. A tumour fragment was fixed for histological examination, which confirmed that the tissue was a renal carcinoma. A further fragment was subjected to short-term culture in order to produce metaphase cells for cytogenetical analysis. Based upon silverstained nucleolar organizing region numbers, 14 of 15 metaphase cells were estimated to have the diploid number (2N = 26) of chromosomes and a karyotype was constructed which did not appear to differ from that of normal cells. A single cell was estimated to be tetraploid (4N = 52). This is the first report of chromosomes of a pronephric Lucké carcinoma. LTV replicates only in tumour tissue maintained in the cold. Because the frog in this study had been maintained in the laboratory at 22 degrees C for about 10 months, no viruses would have been detectable with electron microscopy. However, the presence of Lucké herpesvirus DNA was detected in tumour homogenates by polymerase chain reaction amplification of a 1.2 kbp Hind III restriction fragment of the LTV DNA. The presence of LTV DNA provided assurance that the rescued pronephric tumour was indeed a Lucké carcinoma.     

Poly(adenosine diphosphoribose) polymerase in peripheral blood leukocytes from normal donors and patients with malignancies

A two-color flow cytometric technique was developed to analyze poly(ADP-ribose) polymerase (PADPRP) in different individuals as a function of different physiological or pathological conditions and to establish the basis for determining whether enzyme deficiency may predispose to degenerative or malignant disorders. Peripheral blood granulocytes were devoid of enzyme activity, whereas mononuclear cells had variable amounts. PADPRP was highest in B cells, intermediate in T cells, and lowest in monocytes. This pattern of enzyme distribution and relative enzyme content of different types of cells was remarkably constant in normal subjects. In a series of 66 normal donors there was no significant biological variation in enzyme content as a function of age, race, or sex. The mean PADPRP values in peripheral blood mononuclear cells from 81 random patient samples obtained from an ambulatory oncology clinic did not differ significantly from normal subjects. However, groups of patients with breast cancer, lymphocytic malignancies, and esophageal cancer were observed to have below normal levels for peripheral blood mononuclear cell PADPRP.     

A road map to find in 3D printing a new design plasticity for construction — The state of art

Recent years are showing a rapid adoption of digital manufacturing techniques to the construction industry, with a focus on additive manufacturing. Although 3D printing for construction (3DPC) has notably advanced in recent years, publications on the subject are recent and date a growth in 2019, indicating that it is a promising technology as it enables greater efficiency with fair consumption of material, minimization of waste generation, encouraging the construction industrialization and enhancing and accelerating the constructive process. This new building system not only gives an optimization of the building process but provides a new approach to the building design materiality. The direct connection between design and manufacturing allows the reduction in the number of the various construction phases needed. It is opening a new and wide range of options both formal and chromatic in customization, avoiding complex formworks, reducing costs and manufacturing time. The creative process has a strict and direct link with the constructive process, straightening design with its materiality. Cement-based materials lead the way, but new alternatives are being explored to further reduce its carbon footprint. In order to leverage its sustainability and enhance the system capacity, initiatives are being pursued to allow the reduction of the use of PC. Geopolimers are taking the first steps in 3DPC. Construction and Demolition Waste (CDW) materials are used to substitute natural aggregates. Even soil is being explored has a structural and aesthetic material. These research trends are opening a wider range of possibilities for architecture and design, broadening the spectrum of color, texture, and formal variations. The concern about textures and colours is not yet evident in many the structures already printed, opening the opportunity for future research. More can be done in the mixture and formal design of this building system, “discovering” other raw materials in others waste. This article aims to make a critical review of technologies, materials and methodologies to support the development of new sustainable materials to be used as a plastic element in the printed structure. A roadmap of 3D printing for construction is presented, and an approach on mix design, properties in the fresh and hardened state, highlighting the possibilities for obtaining alternative materials are pointed. With this review possible directions are presented to find solutions to enhance the sustainability of this system discovering “new” materiality for architecture and design.     

Information requirements analysis for holonic manufacturing systems in a virtual environment

The design and development of holonic manufacturing systems requires careful, and sometimes risky, decision making to ensure that they will successfully satisfy the demands of an ever-changing market. In this paper, the authors propose a methodology for a holonic manufacturing systems requirement analysis that is based on a virtual reality approach and aimed at assisting designers of such systems along the entire systems design and development process. Exploiting virtual reality helps the user collect valid information quickly and in a correct form by putting the user and the information support elements in direct relation with the operation of the system in a more realistic environment. A prototype software system tool is designed to realise the features outlined in each phase of the methodology. A virtual manufacturing environment for matching the physical and the information model domains is utilised to delineate the information system requirements of holonic manufacturing systems implementation. A set of rules and a knowledge base is appended to the virtual environment to remove any inconsistency that could arise between the material and the information flows during the requirement analysis.