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11.
Ehrlichia spp. from human and equine sources in the northeastern Unites States were detected by PCR, isolated, and propagated in the HL-60 promyelocytic leukemia cell line. Growth of Ehrlichia from both equine and human sources was enhanced by addition of retinoic acid, which causes granulocytic differentiation of the HL-60 cells. DNA sequencing of a portion of the 16S rDNA gene supported the hypothesis that the same pathogen was responsible for both equine and human granulocytic ehrlichiosis. 相似文献
12.
A chromosomally integrated Bradyrhizobium japonicum hoxA mutant is unable to oxidize hydrogen in free-living conditions. Derepressing conditions that induce hydrogenase activity in free-living, wild-type B. japonicum cells cannot induce expression of the hydrogenase structural genes in the hoxA mutant. The DNA-binding capacity of HoxA at the hup promoter region was studied by means of gel retardation. Both heterotrophically growing cells and cells induced to express hydrogenase activity contain a protein that specifically binds to the hup promoter region. Crude protein extracts isolated from a B. japonicum hoxA mutant do not contain this binding compound. The HoxA protein was overexpressed in E. coli and isolated in the form of a maltose-binding protein (MBP)-HoxA fusion. The MBP-HoxA hybrid protein specifically bound to a 50 bp region of the hupSL promoter known to be important for regulation of hupSL expression. 相似文献
13.
14.
JL Van Laethem M Cremer MO Peny M Delhaye J Devière 《Canadian Metallurgical Quarterly》1998,43(6):747-751
Microcapsules are used for the formulation of drug controlled release and drug targeting dosage forms. Encapsulated hydrophobic drugs are often applied as their solutions in plant oils. The uptake of the oils in the complex coacervate microcapsules can be improved by the addition of surfactants. In this study, soybean, olive and peanut oils were chosen as the representatives of plant oils. The well characterized complex coacervation of gelatin and acacia has been used to produce the microcapsules. The amount of encapsulated oil has been determined gravimetrically. The encapsulation of the oils was high (75-80%). When the surfactants with HLB values from 1.8 to 6.7 were used, the amount of encapsulated oil was high (65-85%). A significant decrease of the oil content in the microcapsules was found when Tween 61 with HLB = 9.6 had been added into the mixture. No oil was found inside the microcapsules from the coacervate emulsion mixture containing Tween 81 (HLB = 10) and Tween 80 (HLB = 15), respectively. The results of the experiment confirm the dependence of hydrophobic substance encapsulation on the HLB published recently for Squalan. 相似文献
15.
International Journal of Computer Vision - 相似文献
16.
Wisse M. Feliksdal G. Van Frankkenhuyzen J. Moyer B. 《Robotics & Automation Magazine, IEEE》2007,14(2):52-62
In this article, we presented our passive-based biped Denise. Using only two foot contact switches as sensors and simple on/off pneumatic muscle action, it walks at 0.4 m/s and can handle floor disturbances up to 6 mm (leg length is 0.7 m). Its simplicity, efficiency, and die natural look of its motions make it a promising lead for the development of commercially viable humanoid robots. The aim of this article is to provide other researchers enough details so that they can reproduce the work or use it as a basis for future research. 相似文献
17.
C. L. J. A. Verbraak J. Van Dam A. K. Van Der Vegt 《Polymer Engineering and Science》1985,25(7):431-433
Orientation-induced crystallization of crystallizable polymer melts can occur, under certain conditions, during flow through converging channels. Attempts have been made to achieve this phenomenon in a two-phase system, i.e., during simultaneous extrusion of a continuous concentric core of polypropylene within a polystyrene matrix through a conical duct. On one occasion, using Carlona P SY6100 (MFI = 11.0) with Hostyren N2000-V-01 (MFI = 25.0), a highly oriented polypropylene thread with a modulus of 14.6 GPa and a melting point of 178°C was extruded at a die temperature of approximately 170°C and a pressure lower than 40 MPa. It is, in principle, possible to form highly oriented, fiber-like structures as reinforcing elements in a polymer matrix. 相似文献
18.
Nagle H.T. Fritzemeier R.R. Van Well J.E. McNamer M.G. 《Industrial Electronics, IEEE Transactions on》1989,36(2):151-163
As the level of microprocessor complexity increases to several hundred thousand transistors for a single-chip machine, it is becoming very difficult to test commercially available designs to the level of fault coverage desired by some customers. In order to achieve near 100-percent coverage of single stuck-at faults, future microprocessors must be designed with special testing features (designed for testability). The authors describe the testing problem for microprocessors, including the various methods of generating test sets and their application by the user. A survey of the testability features of some of today's commercially available microprocessors is presented. Suggestions for testability features for future-generation microprocessors are also discussed 相似文献
19.
Xiaoming Zhou Martin Jacobsson Henk Uijterwaal Piet Van Mieghem 《International Journal of Communication Systems》2008,21(6):643-663
Although packet delay and loss are two important parameters of the Internet performance, to the best of our knowledge, the evolution of large‐scale IPv6 delay and loss performance has previously not been studied. In this paper, we analyze more than 600 end‐to‐end IPv6 paths between about 26 testboxes of RIPE Network Coordination Centre over two years, and compare the delay and loss performance over time with their IPv4 counterparts. We present and discuss the measurement methodologies and show that IPv6 paths have a higher delay and loss than their IPv4 counterparts. The main reason for the worse performance stems from IPv6‐in‐IPv4 tunnels rather than from native IPv6 paths and such tunnels are still widely used today. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献
20.
Recent years have witnessed major governmental initiatives regarding critical infrastructure protection (CIP). During that same time, critical infrastructures (CIs) have undergone massive institutional restructuring under the headings of privatization, deregulation and liberalization. Little research has gone into understanding the interactions between these two developments. In this article, we outline the consequences of institutional restructuring for the changing ways in which CIs ensure the reliability and security of their networks and services. Neither Normal Accident Theory nor High‐Reliability Theory can account for reliability under these conditions. We then investigate the implications of these findings for CIP. 相似文献