In vitro studies on the use of clay, clay minerals and charcoal to adsorb bovine rotavirus and bovine coronavirus

Rotaviruses are the leading cause and coronaviruses are the major contributors of acute gastroenteritis in the young of various mammalian and avian species. Despite numerous trials and decades of research, vaccines have limited efficacy particularly for calves. As an alternative method of controlling infection, we have investigated broad spectrum antiviral agents that are not discriminatory among various viruses. This report involves testing a variety of adsorbent agents including charcoal, clay, and clay minerals to adsorb rotavirus and coronavirus in vitro. Results revealed that all the adsorbent agents had good to excellent capability of adsorbing rotavirus and excellent capability of adsorbing coronavirus. Percent adsorptions ranged from 78.74% to 99.89% for rotavirus and 99.99% for coronavirus; while sand (negative control) was < 0.01%. A high affinity binding was present as determined by a low percent desorption (0.06-3.09%). However, the adsorbent bound virus complex retained, and may have actually enhanced, infectivity.     

The failure of an F/A-18 trailing edge flap hinge

During a flight over sea, the right-hand trailing-edge flap (TEF) from an RAAF F/A-18 separated. Although the flap was not recovered, the mode of failure suggested that the outboard hinge lug was first to fail. A fleet-wide inspection of the TEF outer hinge lugs disclosed two other cracked hinge lugs. Examination of these cracks revealed that they had propagated as the result of a combination of corrosion and fatigue, in that the corrosive environment appeared to have accelerated the fatigue crack growth rate. Laboratory experiments indicated that, in a salt solution, the 7050 aluminium alloy from which the lugs were manufactured is susceptible to corrosion fatigue and that the observed cracking matched that on the cracked lugs: corrosion fatigue was therefore suspected to have been a contributing factor in the unexpected failure. Quantitative fractography on the fatigue cracks, combined with several assumptions and deductions, including information about the failure size of the crack gained from witness marks found on the monoball bearing of the failed TEF, led to an estimate of the fatigue crack growth rate. This crack growth rate and the short service life clearly indicated the limitations of the then-current non-destructive inspection (NDI) technique, and a more sensitive inspection method was developed to assist in the maintenance of adequate RAAF flying hours between inspections. This failure investigation provided an excellent example of how fractography, laboratory experiments and NDI can be combined to determine a possible cause of failure and to establish remedial measures for the fleet.     

A 150 MHz 0.6 μm BiCMOS superscalar microprocessor

An implementation of the Pentium microprocessor architecture in 0.6 μm BiCMOS technology is described. Power dissipation is reduced and performance is enhanced over the previous generation. Processor features, implementation technology, and circuit techniques are discussed. An internal clock rate of 150 MHz is achieved at 3.7 V and -55°C     

Use of a thick-film capillary column for the analysis of organic acids in body fluids

Contradictory evidence as to the effects of alcohol on early information processing stages has been obtained from behavioral and psychophysiological investigations. In the present study, choice reaction times, error rates, and event-related potentials (ERPs) were recorded in a task in which variations in stimulus discriminability and of the (task irrelevant) correspondence between stimulus location and response location were orthogonally combined. Both discriminability and stimulus-response correspondence affected reaction time and electrophysiological chronometric measures as expected. However, no behavioral effects of alcohol were observed, possibly because of strategic adjustments. Psychophysiological chronometric measures indicated that alcohol leaves the initial flow of perceptual evidence to motor stages unimpaired, whereas it appears to increase the duration of stimulus evaluation. Interestingly, a number of alcohol effects appeared in the ERP amplitudes. Decrements in early ERP components indicate alcohol-induced impairments of involuntary visual attention and/or the automatic stimulus location-dependent activation of response channels. In contrast, a strong enhancement of a late slow-wave component under alcohol may reflect the investment of processing resources in order to maintain normal performance levels.     

The hierarchies of knowledge and the mathematics of discovery

Rather than attempting to characterize a relation of confirmation between evidence and theory, epistemology might better consider which methods of forming conjectures from evidence, or of altering beliefs in the light of evidence, are most reliable for getting to the truth. A logical framework for such a study was constructed in the early 1960s by E. Mark Gold and Hilary Putnam. This essay describes some of the results that have been obtained in that framework and their significance for philosophy of science, artificial intelligence, and for normative epistemology when truth is relative.I am indebted to Kevin Kelly for several years of happy conversation from which the perspective and views of this paper grew, for comments on a draft of the paper, and for constructing some of the illustrations. A fellowship from the John Simon Guggenheim Memorial Foundation provided the liberty to write this paper. It was first presented in the Turing Colloquium, 1990.     

Microstructural changes in concretes with sulfate exposure

In prior papers the responses of concretes to 50,000 ppm MgSO₄ exposure depending on cement type, w/cm and the presence of slag were described. The present paper completes this analysis by examining the effects of immersion of concretes produced using slag blended cements, in solutions containing 50,000 ppm of sodium sulfate. The spatial evolution of microstructure associated with carbonation and sulfate attack show differences which can be related to the nature of the cation associated with the sulfate, the cement type, and the w/cm ratio.     

Changes of respiratory chain activity in mitochondrial and synaptosomal fractions isolated from the gerbil brain after graded ischaemia

In this study we have examined (1) the integrated function of the mitochondrial respiratory chain by polarographic measurements and (2) the activities of the respiratory chain complexes I, II-III, and IV as well as the ATP synthase (complex V) in free mitochondria and synaptosomes isolated from gerbil brain, after a 30-min period of graded cerebral ischaemia. These data have been correlated with cerebral blood flow (CBF) values as measured by the hydrogen clearance technique. Integrated functioning of the mitochondrial respiratory chain, using both NAD-linked and FAD-linked substrates, was initially affected at CBF values of approximately 35 ml 100 g-1 min-1, and declined further as the CBF was reduced. The individual mitochondrial respiratory chain complexes, however, showed differences in sensitivity to graded cerebral ischaemia. Complex I activities decreased sharply at blood flows below approximately 30 ml 100 g-1 min-1 (mitochondria and synaptosomes) and complex II-III activities decreased at blood flows below 20 ml 100 g-1 min-1 (mitochondria) and 35-30 ml 100 g-1 min-1 (synaptosomes). Activities declined further as CBF was reduced below these levels. Complex V activity was significantly affected only when the blood flow was reduced below 15-10 ml 100 g-1 min-1 (mitochondria and synaptosomes). In contrast, complex IV activity was unaffected by graded cerebral ischaemia, even at very low CBF levels.