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81.
Salicylate-containing phenolic glycosides (PGs) are abundant and often play a dominant role in plant-herbivore interactions of Populus and Salix species (family Salicaceae), but the biosynthetic pathway to PGs remains unclear. Cinnamic acid (CA) is thought to be a precursor of the salicyl moiety of PGs. However, the origin of the 6-hydroxy-2-cyclohexen-on-oyl (HCH) moiety found in certain PGs, such as salicortin, is not known. HCH is of interest because it confers toxicity and antifeedant properties against herbivores. We incubated Populus nigra leaf tissue with stable isotope-labeled CA, benzoates, and salicylates, and measured isotopic incorporation levels into both salicin, the simplest PG, and salicortin. Labeling of salicortin from [13C6]-CA provided the first evidence that HCH, like the salicyl moiety, is a phenylpropanoid derivative. Benzoic acid and benzaldehyde also labeled both salicyl and HCH, while benzyl alcohol labeled only the salicyl moiety in salicortin. Co-administration of unlabeled benzoates with [13C6]-CA confirmed their contribution to the biosynthesis of the salicyl but not the HCH moiety of salicortin. These data suggest that benzoate interconversions may modulate partitioning of phenylpropanoids to salicyl and HCH moieties, and hence toxicity of PGs. Surprisingly, labeled salicyl alcohol and salicylaldehyde were readily converted to salicin, but did not result in labeled salicortin. Co-administration of unlabeled salicylates with labeled CA suggested that salicyl alcohol and salicylaldehyde may have inhibited salicortin biosynthesis. A revised metabolic grid model of PG biosynthesis in Populus is proposed, providing a guide for functional genomic analysis of the PG biosynthetic pathway. 相似文献
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Variable population size and evolution acceleration: a case study with a parallel evolutionary algorithm 总被引:1,自引:0,他引:1
Ting Hu Simon Harding Wolfgang Banzhaf 《Genetic Programming and Evolvable Machines》2010,11(2):205-225
With current developments of parallel and distributed computing, evolutionary algorithms have benefited considerably from parallelization techniques. Besides improved computation efficiency, parallelization may bring about innovation to many aspects of evolutionary algorithms. In this article, we focus on the effect of variable population size on accelerating evolution in the context of a parallel evolutionary algorithm. In nature it is observed that dramatic variations of population size have considerable impact on evolution. Interestingly, the property of variable population size here arises implicitly and naturally from the algorithm rather than through intentional design. To investigate the effect of variable population size in such a parallel algorithm, evolution dynamics, including fitness progression and population diversity variation, are analyzed. Further, this parallel algorithm is compared to a conventional fixed-population-size genetic algorithm. We observe that the dramatic changes in population size allow evolution to accelerate. 相似文献
84.
Simon Harding Julian F. Miller Wolfgang Banzhaf 《Genetic Programming and Evolvable Machines》2010,11(3-4):397-439
Self-modifying Cartesian Genetic Programming (SMCGP) is a general purpose, graph-based, developmental form of Genetic Programming founded on Cartesian Genetic Programming. In addition to the usual computational functions, it includes functions that can modify the program encoded in the genotype. This means that programs can be iterated to produce an infinite sequence of programs (phenotypes) from a single evolved genotype. It also allows programs to acquire more inputs and produce more outputs during this iteration. We discuss how SMCGP can be used and the results obtained in several different problem domains, including digital circuits, generation of patterns and sequences, and mathematical problems. We find that SMCGP can efficiently solve all the problems studied. In addition, we prove mathematically that evolved programs can provide general solutions to a number of problems: n-input even-parity, n-input adder, and sequence approximation to π. 相似文献
85.
高性能玻璃纤维增强材料 总被引:1,自引:1,他引:1
林树益 《玻璃钢/复合材料》1996,(1):8-12
本文简要评述了国内外高性能玻纤增强材料如高强度、高模量、高硅氧、抗辐照、抗碱、空心玻璃纤维等的研究进展与现状;同时评述了高新玻纤制品的现状及其应用,如连续原丝毡、针织缝编毡、3D织物、复铜板薄毡、膨体纱系列产品、增强热塑性塑料玻纤基材等。 相似文献
86.
Preparation and characterization of chitosan films,crosslinked with symmetric aromatic dianhydrides to achieve enhanced thermal properties 下载免费PDF全文
Iman Kavianinia Paul G Plieger Nadia G Kandile David RK Harding 《Polymer International》2015,64(4):556-562
This study has developed a new generation of crosslinked chitosan‐based films using symmetric aromatic dianhydrides as crosslinking agents. The formation of the dianhydride‐crosslinked chitosan hydrogel films was confirmed using Fourier transform infrared and solid‐state 13C NMR spectral analyses. The films obtained from these derivatives were characterized by their thermal, swelling and hydrophilic properties. The results showed that introducing a cyclic imide moiety into the chitosan matrices played a significant role in enhancing the thermal properties of these chitosan films. It was found that even at high levels of substitution, thermal stability of the studied chitosan derivatives was improved, in spite of a reduction in crystallinity. Heterocyclic imide linkages produced networks that were insoluble in both acidic and alkaline media but allowed swelling in aqueous media. An increase in the hydrophobicity of the chitosan film surfaces was observed after introduction of the cyclic imide moiety. These engineered films produced noteworthy results concerning their thermal and swelling properties. There is a need to further investigate these films for drug delivery and biomaterials applications. © 2014 Society of Chemical Industry 相似文献
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88.
Solomon A. Mensah Ian C. Harding Michelle Zhang Michael P. Jaeggli Vladimir P. Torchilin Mark J. Niedre Eno E. Ebong 《American Institute of Chemical Engineers》2019,65(8):e16634
While it is known that cancer cell interactions with vascular endothelial cells (ECs) drive metastatic cancer cell extravasation from blood vessels into secondary tumor sites, the mechanisms of action are still poorly understood. Here, we tested the hypothesis that neuraminidase-induced degradation of EC surface glycocalyx (GCX), particularly the sialic acid (SA) residue components of the GCX, will substantially increase metastatic cancer cell attachment to ECs. To our knowledge, our study is the first to isolate the role of GCX SA residues in cancer cell attachment to the endothelium, which were found to be differentially affected by the presence of neuraminidase and to indeed regulate metastatic cancer cell homing to ECs. We hope that this work will eventually translate to identification of EC GCX-based cancer markers that can be therapeutically targeted to hinder the progression of metastasis. 相似文献
89.
MM Sheehan E Stanley GF Fitzgerald D van Sinderen 《Canadian Metallurgical Quarterly》1999,65(2):569-577
A lysis module encoded by the temperate bacteriophage phiO1205 was identified. This lysis module contains a lysin gene, designated lyt51, and two putative holin-encoding genes, designated lyt49 and lyt50. lyt51 encodes a lytic enzyme specifically directed against streptococcal cell walls. Similar to other phage-encoded lysins, Lyt51 appears to have a modular design in which the N-terminal portion corresponds to its enzymatic activity while the C-terminal region is responsible for its substrate binding specificity. The two putative holin-encoding genes, lyt49 and lyt50, located immediately upstream of lyt51, were identified on the basis of their homology to other identified holin-encoding genes. Expression of lyt49 or lyt50 in Escherichia coli was shown to cause cell death and leakage of the intracellular enzyme isocitrate dehydrogenase into the growth medium without apparent lysis of the cells. Southern blotting experiments demonstrated that at least one of the three components of the identified lysis module is present in all members of a large collection of bacteriophages, indicating that components of this lysis module are widespread among bacteriophages infecting Streptococcus thermophilus. 相似文献
90.