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81.
L. Bourouiba Jianhong Wu S. Newman J. Takekawa T. Natdorj N. Batbayar C. M. Bishop L. A. Hawkes P. J. Butler M. Wikelski 《Journal of the Royal Society Interface》2010,7(52):1627-1639
Virulent outbreaks of highly pathogenic avian influenza (HPAI) since 2005 have raised the question about the roles of migratory and wild birds in the transmission of HPAI. Despite increased monitoring, the role of wild waterfowl as the primary source of the highly pathogenic H5N1 has not been clearly established. The impact of outbreaks of HPAI among species of wild birds which are already endangered can nevertheless have devastating consequences for the local and non-local ecology where migratory species are established. Understanding the entangled dynamics of migration and the disease dynamics will be key to prevention and control measures for humans, migratory birds and poultry. Here, we present a spatial dynamic model of seasonal migration derived from first principles and linking the local dynamics during migratory stopovers to the larger scale migratory routes. We discuss the effect of repeated epizootic at specific migratory stopovers for bar-headed geese (Anser indicus). We find that repeated deadly outbreaks of H5N1 on stopovers during the autumn migration of bar-headed geese could lead to a larger reduction in the size of the equilibrium bird population compared with that obtained after repeated outbreaks during the spring migration. However, the opposite is true during the first few years of transition to such an equilibrium. The age-maturation process of juvenile birds which are more susceptible to H5N1 reinforces this result. 相似文献
82.
We have used comparative sequence analysis to identify an intein-like sequence (protein splicing element) present in Cryptococcus neoformans, a fungal pathogen of humans. The sequence encoding this element is present in the C. neoformans PRP8 gene, as an in-frame insertion relative to the PRP8 genes of other organisms. It contains sequences similar to those of the protein-splicing domains of two previously described yeast inteins (in Saccharomyces cerevisiae and Candida tropicalis), although it lacks any recognizable internal endonuclease domain. The Cryptococcus neoformans intein (Cne PRP8) is only the second to be found in a eukaryote nuclear genome; the previously described yeast inteins occur at the same site in the VMA gene homologues of S. cerevisiae and C. tropicalis. The host gene of the Cryptococcus intein, PRP8, encodes a highly conserved mRNA splicing protein found as part of the spliceosome. The Cne PRP8 intein may be a useful drug target in addressing the cryptococcal infections so prevalent in AIDS patients. 相似文献
83.
Assessment of gully pot management strategies for runoff quality control using a dynamic model 总被引:1,自引:0,他引:1
A gully pot is the first entry point of road runoff into an urban drainage network. Pots are extensively used to trap solids from runoff in order to avoid/minimise the problems associated with sediment deposition in the downstream drainage structures or receiving waters. Here we briefly describe the different modules of a dynamic gully pot model developed to assess the impact of a series of management practice scenarios on the quality of runoff discharged through the pots. Runoff quality was modelled in terms of suspended solids (SS), chemical oxygen demand (COD), dissolved oxygen (DO) and ammonium. The model has the flexibility to represent the impact of various interactions between physical and biochemical processes occurring in a pot during wet and dry weather conditions, respectively. The simulation results show that the pots are effective at retaining solids, but their role with regard to reduction of dissolved pollutants is almost neutral. Model predictions, as against common perception, show that frequent pot cleaning does not significantly improve the runoff quality. However, considerably improved solid retention is possible if larger pots with modified geometry are introduced into the drainage system. 相似文献
84.
In collaboration with the Armed Forces Institute of Pathology's Department of Defense DNA Registry, the National Institute of Standards and Technology recently evaluated the performance of a short tandem repeat multiplex with dried whole blood stains on four different commercially available identification card matrixes. DNA from 70 stains that had been stored for 19 months at ambient temperature was extracted or directly amplified and then processed using routine methods. All four storage media provided fully typeable (qualitatively identical) samples. After standardization, the average among-locus fluorescence intensity (electropherographic peak height or area) provided a suitable metric for quantitative analysis of the relative amounts of amplifiable DNA in an archived sample. The amounts of DNA in Chelex extracts from stains on two untreated high-purity cotton linter pulp papers and a paper treated with a DNA-binding coating were essentially identical. Average intensities for the aqueous extracts from a paper treated with a DNA-releasing coating were somewhat lower but also somewhat less variable than for the Chelex extracts. Average intensities of directly amplified punches of the DNA-binding paper were much larger but somewhat more variable than the Chelex extracts. Approximately 25% of the observed variation among the intensity measurements is shared among the four media and thus can be attributed to intrinsic variation in white blood count among the donors. All of the evaluated media adequately "bank" forensically useful DNA in well-dried whole blood stains for at least 19 months at ambient temperature. 相似文献
85.
86.
The effects of cavities located near the base of a ground plane-mounted coax-driven monopole antenna are investigated. The cavities are either embedded in the tubular antenna itself or in the region below the conducting surface. Coupled integral equations are formulated and solved to determine the distribution of current on the structure. Two primary functions of the cavities are discussed. 1) At certain frequencies the input impedance of the cavity is infinite and the current at the base of the antenna is forced to zero. A single structure may contain several cavities, each cavity having unique resonant frequencies. It is demonstrated that superposition of these cavities may provide multiple resonant frequencies within a large frequency band. The utility of base cavities in the design of an antenna that is decoupled from the ground plane on which it is mounted is discussed. 2) Regardless of frequency, the cavities affect the distribution of current on the structure and, concomitantly, the standing wave ratio and gain of the antenna. The cavities are shown to be efficient and physically-rigid tools for current-shaping and tuning coax-fed antennas. Results computed from solutions of the coupled integral equations are compared with those from physical measurements. 相似文献
87.
Nai-Hsiang Sun Chih-Cheng Chou Hung-Wen Chang Butler J.K. Evans G.A. 《Lightwave Technology, Journal of》2006,24(6):2409-2415
The Floquet-Bloch theory (FBT) is used to determine the radiation loss of a grating-assisted directional coupler. Improper waves obtained by FBT represent leakage and produce radiation loss along the transverse direction. By summing the fields of all leaky harmonics, the power flow radiated into the superstrate and substrate can be calculated from Poynting's theorem. However, the locations at which these summations occur impact the accuracy. An analytic formula for radiation loss is also derived. Results from both the FBT and the analytic formula are compared with results obtained using the coupled-mode theory. 相似文献
88.
89.
Butler Stephen F.; Newman Frederick L.; Cacciola John S.; Frank Arlene; Budman Simon H.; McLellan A. Thomas; Ford Sabrina; Blaine Jack; Gastfriend David; Moras Karla; Salloum Ihsan M.; Barber Jacques P. 《Canadian Metallurgical Quarterly》1998,10(4):399
The Addiction Severity Index (ASI) is a reliable and valid measure of problem severity among addicted patients. Concerns have been raised about the reliability of the Interviewer Severity Rating (ISR), a summary score for each of 7 domains. As part of an effort to build a computer-administered ASI, regression equations were developed to predict the ISR. Repeated resampling of a large dataset, consisting of 1,124 ASIs conducted by trained interviewers, permitted derivation of stable regression equations predicting the ISR for each ASI domain from patients' answers to preselected interview items. The resulting 7 Predicted Severity Ratings (PSRs) were tested on 8, standardized vignettes, with "gold standard," expert-generated ISRs. Reliabilities compared well with those of intensively trained interviewers. The PSRs could provide an alternative to potentially unreliable interviewer ratings, enhancing the ASI's role in treatment planning and treatment matching and make possible a computer-administered version of the ASI. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
90.
Bioassay-directed fractionation of an EtOH extract of Curcuma zedoaria led to isolation of an active curcuminoid, which was identified as demethoxycurcumin (2) by comparison of its 1H and 13C NMR spectra with literature data and by direct comparison with synthetic material. Curcumin (1) and bisdemethoxycurcumin (3) were also obtained. Curcuminoids (1-3) were synthesized and demonstrated to be cytotoxic against human ovarian cancer OVCAR-3 cells. The observed CD50 values of 1, 2, and 3 were 4.4, 3.8, and 3.1 microg/mL, respectively. Three additional novel compounds, 3, 7-dimethylindan-5-carboxylic acid (4), curcolonol (5), and guaidiol (6), were also isolated from the EtOH extract. The structures and relative stereochemistry of 4-6 were determined by spectroscopic methods and X-ray crystallographic analysis. 相似文献