Sonography and quantitative measurements

A preliminary study of coronary artery wall topography and mechanical properties is presented. The aim of this study was to give a brief demonstration of scanning acoustic microscopy (SAM) as a sonographic technique, and to apply the time-resolved SAM (TR-SAM) technique for quantification of coronary artery wall mechanical properties under passive conditions ex-vivo, and compare the data for the tunica externa and tunica media of the wall. The authors chose the diagonal branches of the left anterior descending coronary artery (LADCA) of young healthy pigs for measurements. It is concluded that SAM is well suited for sonography at the micrometer level, and TR-SAM provides a refined tool for biorheological quantification ex-vivo, provided that a number of physical factors influencing measurements and tissue properties are considered and dealt with. With time and effort, SAM may also become a valuable tool for recognizing important relations of composition and structure to function. For future SAM studies of arteries, more detailed analyses of layer interfaces and better models of biorheology should be applied to describe the anisotropy and nonlinear viscoelasticity of the wall     

Ether lipids and platelet-activating factor: evolution and cellular function

This review considers the relation between the evolution of ether lipids and platelet-activating factor (PAF) in living organisms for the first time. Ether lipids are shown to be the main structural lipid components in the cells of the most primitive organisms on the Earth; during evolution they were gradually substituted for lipids with ester and vinyl bonds. Synthesis of PAF has been found in some bacteria, protozoans, yeasts, plants, marine invertebrates, lower vertebrates, and mammals. The regulatory role of PAF is suggested to already appear in protozoans and later be maintained during the subsequent evolution of living organisms. During evolution, functions of PAF in the cell have been changing and enlarging, while ether lipids have been gradually losing their role as the main structural lipid component of the cells of living organisms.     

Detection of antineutrophil cytoplasmic antibodies in primary sclerosing cholangitis: a comparison of the alkaline phosphatase and immunofluorescent techniques

SSCP (single-strand conformational polymorphism) is used widely in the field of human biomedicine, but its potential as a population genetics tool for the recovery of nuclear gene genealogies remains to be realized. We describe and illustrate a use for SSCP in the physical isolation of nuclear haplotypes that circumvents several difficulties associated with more conventional cloning procedures. The DNA sequence can be determined directly from the isolated haplotypes and used for phylogenetic inference. SSCP provides a convenient first step toward generating nuclear genealogies for population studies.     

Intraoperative monitoring of the motor pathway using transtracheal stimulation of the cervical spine in dogs

Although SEP monitoring of the spinal cord has been a well established method recently, not an ultimate, perfectly developed technique for monitoring of the motor system is known so far, particularly, because of the disturbing effect of narcotic drugs and relaxants on the motor evoked potentials. In this study the upper part of the spinal cord was stimulated in 14 anesthetized and relaxed dogs with a cathode attached to the intratracheal tube and an anode fixed to the cervical spinous processes. Single and serial stimuli were applied. Recordings were obtained from the exposed right femoral nerve and quadriceps muscle. Averaging was necessary when using serial stimulations. Responses were consequent and reproducible during regular anesthesia. The origin of the different responses in the spinal cord is discussed. The method seems to be appropriate for intraoperative monitoring of the thoracolumbar spine.     

Practical guidelines for expert-judgment-based software effort estimation

This article presents seven guidelines for producing realistic software development effort estimates. The guidelines derive from industrial experience and empirical studies. While many other guidelines exist for software effort estimation, these guidelines differ from them in three ways: 1) They base estimates on expert judgments rather than models. 2) They are easy to implement. 3) They use the most recent findings regarding judgment-based effort estimation. Estimating effort on the basis of expert judgment is the most common approach today, and the decision to use such processes instead of formal estimation models shouldn't be surprising. Simple process changes such as reframing questions can lead to more realistic estimates of software development efforts.     

Airway versus transbronchial biopsy and BAL in lung transplant recipients: different but complementary

Lung transplantation is now an established therapeutic intervention for end-stage cardiopulmonary disease in humans. Chronic rejection, in the form of bronchiolitis obliterans syndrome (BOS), remains the commonest cause of morbidity and mortality in those surviving more than 3 months. The pathology of BOS involves airway changes. We have evaluated the potential for endobronchial biopsies (EBB) to complement existing sampling methods used in allograft monitoring and have compared the results of EBB findings with those of bronchoalveolar lavage (BAL) and transbronchial biopsy (TBB) in 18 clinically stable patients. We found that all the EBB had inflammatory cells present but that only five TBB specimens had evidence of inflammation, with airway material being present in 78% of the TBB. Paired BAL and EBB yielded different results, with no correlations between total macrophages, lymphocytes, CD4+ cells or CD8+ cells. We conclude that endobronchial biopsies are potentially useful as an additional sample for the monitoring of inflammation in lung allografts, since they yield different, and potentially complimentary, information to bronchoalveolar lavage and transbronchial biopsy.     

Comparative assessment of solar concentrator materials

This paper reports results from long-term durability tests of reflector materials to be used for solar concentrating systems. The studies have been conducted under the auspices of an IEA–SolarPACES collaboration between the National Renewable Energy Laboratory (NREL, USA), the Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas (CIEMAT, Spain) and Deutsches Zentrum für Luft- und Raumfahrt (DLR, Germany). In this co-operative effort, accelerated ageing tests as well as outdoor exposures at a number of test sites having various climatic conditions have been carried out since 1995. In addition to materials already in use at solar power stations, newer materials offering the chance of a significant cost reduction in solar electricity and process heat generation are being investigated. Comparative optical tests are carried out to assess the efficiency as a function of exposure/service time in a solar concentrator. Among the materials showing promise for long-term outdoor applications are various silvered glass mirrors, a silvered polymer film, and an anodized sheet aluminium having an additional protective polymer coating. In addition to durability tests of reflector material samples, practical results are also reported for experiences with field applications of silvered thin glass and anodized sheet aluminium mirrors.     

Laboratory issues in the detection and reporting of antibacterial resistance

The emergence of antimicrobial resistance among several common bacterial pathogens requires that clinical microbiology laboratories have the ability to promptly and accurately recognize resistance in patients' isolates. Laboratories have several options for performing routine susceptibility testing, including the broth microdilution procedure (with or without instrumentation for test reading), automated instrument systems that provide rapid results, antibiotic gradient diffusion, and disk diffusion procedures. In addition, there are definitive screening tests capable of recognizing resistance to drugs of choice among several common bacterial species based on single drug concentration tests or rapid spot tests. The likely emergence of still newer resistance mechanisms will provide a challenge to clinical microbiologists to devise accurate, yet cost-effective strategies for use in the future.