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Immobilization was carried out of the lactate dehydrogenase (LDH) from rabbit muscle (EC 1.1.1.27), cross-linked through the bifunctional reactive glutar-aldehyde on to nylon tubing (1 m long, 53cm2 internal surface area). Immobilized LDH inactivation kinetics are of first order (t1/2 = 3·6 years, k = 5·4,e?4 day?1 to 5°C). The smaller effect of pH on activity than in the case of LDH in solution can be explained on the basis of limitation to proton diffusion towards the support. A limiting effect to free external diffusion of the substrate towards and products from the support was also observed, an effect which seems to determine the effective kinetic behaviour of immobilized LDH. The apparent optimum temperature is centred around 40°C, observing a clear inactivation (thermal denaturation) above this temperature. In the temperature range studied (10–40°C), the co-existence was seen of a kinetic control accompanied by another control, involving diffusional transport of substrates and products, on the global activity of the immobilized enzyme. This makes the Arrhenius profiles curvilinear. Both graphic and statistical non-linear regression analysis of the kinetic data—rate, v, versus substrate concentration [S]—carried out under conditions in which the diffusional limitations can be considered negligible (high recirculation flow rate), permitted investigation of the intrinsic kinetic behaviour of immobilized LDH. In this sense, it can be deduced that the rate equation to which these data seem to be fitted is of the polynomial quotient type in [S] of minimum degree 2:2. Although the diffusional limitations have a marked effect on the type of global kinetics shown by immobilized LDH, temperature was not found to affect its v[S] behaviour. The experimental evidence obtained thus indicates that the rate equation in the 10-40°C temperature range continues to be a rational equation of at least degree 2:2 in [S]. 相似文献
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GJ del Zoppo 《Canadian Metallurgical Quarterly》1995,333(24):1632-1633
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Henk A. van der Schee Willy B. H. Kennedy Jan-Paul Bouwknegt Renske Hittenhausen-Gelderblom 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1989,188(1):11-15
Summary Linear discriminant analysis of GLC-aromagram data is used to separate several classes of wine distillates, namely cognac, armagnac and brandy. In this paper we demonstrate how this method was used to detect adulteration. The adulteration was confirmed by the presence of ethyl heptanoate and limonene, which are well-known flavour compounds. They were analysed by means of a Purge and Trap headspace technique. Mass spectrometry was used to identify these flavours.
Ein Beispiel für die Verfälschung von Cognac
Zusammenfassung Die Linear-Discriminanz-Analyse von GLC-Aromagramm-Daten wird zur Klassifizierung von Weindestillaten wie Cognac, Armagnac und Brandy verwendet. In dieser Arbeit werden mit dieser Methode Verfälschungen nachgewiesen, die durch die Gegenwart der Ethylester der Heptansäure und des Limonen belegt werden konnten. Diese Substanzen wurden mit der Purge- und Trap-Methode erfaßt und massenspektrometrisch identifiziert.相似文献
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Polymer Bulletin - Copolymerization of α-(p-vinylphenyl) polyisobutylene(p-polyisobutenylstyrene, PIB-St) with styrene (St) in emulsion has been investigated. Copolymerization occurs only when... 相似文献
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SI Chowdhury BJ Lee D Mosier JH Sur FA Osorio G Kennedy ML Weiss 《Canadian Metallurgical Quarterly》1997,117(4):295-310
CI-994 (acetyldinaline) is an orally active anticancer drug currently in Phase 1 clinical trials. To assess its preclinical toxicity, CI-994 was administered orally as suspensions to Wistar rats (10/sex/dose) and in capsules to beagle dogs (3/sex/dose) once daily for two weeks. Doses were 1.5, 5, and 15 mg/kg for rats (9, 30, and 90 mg/m2, respectively), and 0.5, 2, and 5 mg/kg for dogs (10, 40, and 100 mg/m2, respectively). Systemic exposure was dose-proportional based on toxicokinetic analysis in dogs. Severe clinical signs and mortality occurred at the highest dose in both species beginning on Day 10. Neutropenia, lymphocytopenia, thrombocytopenia, lymphoid depletion, bone marrow hypocellularity, and testicular degeneration were observed in both species, primarily at the mid- and high-doses. Despite continued treatment, neutrophil counts in dogs returned to control levels in Week 2. Other microscopic findings in rats included splenic hematopoietic depletion at all doses and epithelial cell necrosis in various tissues at 15 mg/kg. Additional bone marrow changes in dogs involved myeloid and megakaryocyte hyperplasia at 2 mg/kg and abnormal myeloid and megakaryocyte maturation at 2 and 5 mg/kg. Except for the testicular effects in both species, all changes were reversible within a 4-week (rat) or 9-week (dog) recovery period. The results of these studies show that target organ effects of CI-994 principally involve tissues with rapidly dividing cell populations and that bone marrow suppression is the dose-limiting toxicity. CI-994 also seems to interfere with the release and/or maturation of cells in the bone marrow. 相似文献
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