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71.
EJ Velthuis-te Wierik H van den Berg JA Weststrate KH van het Hof C de Graaf 《Canadian Metallurgical Quarterly》1996,50(4):214-219
Plasma levels of fibrinogen, factor VIIc and prothrombin fragment F1 + 2, a marker of thrombin generation in vivo, were studied in 68 subjects with serum total cholesterol (TC) levels between 135 and 349 mg/dl but without clinical evidence of cardiovascular disease and other atherosclerotic risk factors. F1 + 2 plasma levels were directly correlated with TC (p < 0.0004), low-density lipoprotein cholesterol (LDL-C; p < 0.0018) and factor VIIc (p < 0.024). Thirty-five subjects with TC greater than 249 mg/dl (median value of the whole group) showed higher levels of F1 + 2 (p < 0.0001) and fibrinogen (p < 0.0015) than those with TC lower than 249 mg/dl. In subjects with TC > 249 mg/dl and F1 + 2 > 1.2 nM (median value of the whole group), a cholesterol-lowering drug (simvastatin) was able to reduce F1 + 2 (p < 0.009) as well as TC and LDL-C. This study shows a relationship between serum cholesterol and the rate of thrombin generation supporting the hypothesis that a hypercoagulable state may occur in hypercholesterolemic subjects before the onset of clinical evidence of atherosclerotic cardiovascular disease. 相似文献
72.
A review of 35 patients > 60 years of age with odontoid fractures showed that a type II injury (Anderson and D'Alonso) was the most common fracture pattern (82% of cases). On the basis of mechanism of injury and sex incidence, we deduced that osteopenia is a contributory factor in the occurrence of odontoid fractures. A fall in a domestic setting was the cause in 53%. The peg was posteriorly displaced in 88% of type II fractures, and primary union occurred in 23%. The incidence of concomitant spinal cord injury with type II fractures was higher in older patients than it was in those < 60 years of age. The outcome for these patients was largely determined by their neurological status at presentation. Myelopathy as a late complication of nonunion was not observed in nine patients with an average follow-up of 21 months. The data suggest that vigorous attempts to secure both primary union and a sound arthrodesis for non-union are questionable in the elderly except in unusual circumstances. 相似文献
73.
Gene amplification in a p53-deficient cell line requires cell cycle progression under conditions that generate DNA breakage 总被引:1,自引:0,他引:1
Amplification of genes involved in signal transduction and cell cycle control occurs in a significant fraction of human cancers. Loss of p53 function has been proposed to enable cells with gene amplification to arise spontaneously during growth in vitro. However, this conclusion derives from studies employing the UMP synthesis inhibitor N-phosphonacetyl-L-aspartate (PALA), which, in addition to selecting for cells containing extra copies of the CAD locus, enables p53-deficient cells to enter S phase and acquire the DNA breaks that initiate the amplification process. Thus, it has not been possible to determine if gene amplification occurs spontaneously or results from the inductive effects of the selective agent. The studies reported here assess whether p53 deficiency leads to spontaneous genetic instability by comparing cell cycle responses and amplification frequencies of the human fibrosarcoma cell line HT1080 when treated with PALA or with methotrexate, an antifolate that, under the conditions used, should not generate DNA breaks. p53-deficient HT1080 cells generated PALA-resistant variants containing amplified CAD genes at a frequency of >10(-5). By contrast, methotrexate selection did not result in resistant cells at a detectable frequency (<10(-9)). However, growth of HT1080 cells under conditions that induced DNA breakage prior to selection generated methotrexate-resistant clones containing amplified dihydrofolate reductase sequences at a high frequency. These data demonstrate that, under standard growth conditions, p53 loss is not sufficient to enable cells to produce the DNA breaks that initiate amplification. We propose that p53-deficient cells must proceed through S phase under conditions that induce DNA breakage for genetic instability to occur. 相似文献
74.
Solution of temperature fields in hydrodynamics bearings by the numerical network method 总被引:1,自引:0,他引:1
Solution to the 2-D steady-state nonlinear heat conduction equation, involving cylindrical coordinates, applied to a plain bearing by a simple and versatile numerical technique based on network method is presented in this work. These advantages of the technique are necessary for the status evaluation of industrial machines during operation because time and computing resources are limited. The pressure field in the fluid is previously solved from the Reynolds equation by finite element method. The main difficulty is associated with the exponential dependency of the viscosity on temperature. The proposed model is very efficient and requires negligible computing times. Solutions are very close to the experimental and numerical results of other researchers. 相似文献
75.
Cristiane Vieira Helm Alicia de Francisco Salete Aparecida Gaziola Ricardo Francisco Fornazier Georgia Bertoni Pompeu Ricardo Antunes Azevedo 《Food Biotechnology》2005,18(3):327-341
Barley (Hordeum vulgare L.) has had an important impact on human nutrition. Hull-less barley is a genetically improved type that has been widely used in recent years. Six Brazilian hull-less barley varieties (IAC-IBON 214-82; IAC 8612-421; IAC 8501-31; IAC 8501-12; IAPAR 39-ACUMAI; IAC 8501-22) were analyzed for storage protein constituents, amino acid contents, and similarity among the hull-less barley varieties. Albumins, globulins, prolamins I and II, and glutelins were extracted and separated by SDS-PAGE. The total protein amino acid contents of the flour were also determined for each variety by TLC and HPLC. Variations in intensity and appearance and disappearance of protein bands were observed among the varieties suggesting genetic variability. However, the amino acid profile did not indicate any major variations in the amino acid concentrations. The high lysine and threonine total concentrations detected in the seeds of the hull-less barley varieties encouraged an investigation into the regulation of amino acid metabolism and storage protein synthesis. 相似文献
76.
We describe a method for computing solutions of multi-point boundary value problems, where the polychotomic structure of the underlying solution space is specifically exploited. After the system is integrated in a multiple shooting way, the resulting linear system is solved by a special decoupling algorithm that takes into account the fact that the dichotomy on each interval is potentially different (in contrast to the two point case). A number of examples sustains the theory. 相似文献
77.
Jing Wang Walker D.M. Xiang Lu Majhi A. Kruseman B. Gronthoud G. Villagra L.E. van de Wiel P.J.A. Eichenberger S. 《Design & Test of Computers, IEEE》2007,24(3):226-234
Excessive power supply noise during test can cause overkill. This article discusses two models for supply noise in delay testing and their application to test compaction. The proposed noise models avoid complicated power network analysis, making them much faster than existing power noise analysis tools. can cause performance degradation and 相似文献
78.
Hemagglutinins present in Amaranthus leucocarpus seed meal were extracted with saline buffer and nutritive value was estimated by PER, NPR as well as Digestible Energy Consumption Evaluations. Protein digestibility was determined both in vitro and in vivo. Trypsin inhibitor activity was also determined. Saline-extracted Amaranth meal had significantly higher PER and NPR values than whole meal (p<0.05). There were no significant differences in digestible energy (Kcal/day/ animal) and protein consumption (g/day/animal) between diets (p<0.05). A. leucocarpus improves in nutritive value after the saline extraction procedures and has a good potential as a complementary food due to its lysine content. In addition purified agglutinin may be a useful biochemical. 相似文献
79.
Transport problems typically involve at least two types of constraints, on income and on time. Therefore, the indirect utility
function depends either on the income available after having subtracted the cost of the discrete alternative and on the free
time left after having worked and travelled by each competing option. In the typical linear-in-the-attributes and in-the-parameters
specification, that represents the first grade approximation of the indirect utility function, the effect of income and time
constraints cancel out and only the cost and time of the alternatives matter in the comparison between them. From a microeconomic
point of view this is equivalent to assume that income and time effects could be disregarded; which is not always the case.
To account for these effects the utility function should include second order attributes; however, in non-linear utility functions
it may not be easy to distinguish among several effects that could be relevant: direct preferences for good and leisure, and
simple interactions between attributes other than income and time effects. This paper analyses these effects from a theoretical
point of view focusing on the possible confounding problem in detecting income and time effects. We use a dataset collected
for a modal choice context and containing both revealed and stated preference data, and estimate several NL models examining
the effect of the different second-order terms on detecting income and time effects. We compared specifications including
square cost and time attributes, interactions between time and cost, cost divided by the income available to be spent on free
time, and time multiplied by free time. Our results confirm the strong effect of direct preferences for goods and leisure
time on choice, and the potential confounding effect between quadratic attributes and other non-linear omitted terms. Finally,
we also found that care should be taken in highlighting income and time effects using mixed data sources, since confounding
effects can occur when non-linearities are accounted for in both data sets. 相似文献
80.