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101.
Mongi Jemmali Talluri R. K. Murthy 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1976,265(5):13-17
A method is presented for the determination of aflatoxins in animal tissues. It consists of extraction of aflatoxins from the sample with methanol, treatment of the residue with a mixture of dimethoxymethane-methanol (4 + 1) to further precipitate proteins, evaporation of dimethoxymethane followed by a liquid-liquid defatting with hexane and heating of the methanol-water mixture before the transfer of aflatoxins into chloroform. Chloroform extracts are further purified by silica gel—acidic alumina—anhydrous sodium sulfate column chromatography. 相似文献
102.
The orientation of the noncrystalline (amorphous) phase in poly(ethylene terephthalate) fibers is analyzed by separating the amorphous scattering in the wide-angle x-ray diffraction patterns into isotropic and anisotropic components. Two parameters are used to characterize the amorphous orientation—the fraction of the anisotropic component and its degree of orientation. The x-ray amorphous orientation parameters are compared with the sonic modulus and the birefringence values. Our results illustrate that the intrinsic birefringence of the amorphous phase is not “intrinsic” but depends on its density. The role of the oriented amorphous phase in determining the strength (tenacity) and the dimensional stability (shrinkage) of the fibers is discussed. We conclude that although amorphous orientation determines the shrinkage, other factors such as the connectivity between the amorphous phase and the crystalline regions play an important role in determining the tenacity of the fibers. © 1997 John Wiley & Sons, Inc. J Appl Polym Sci 64: 1363–1371, 1997 相似文献
103.
EM Silini F Bosi NS Pellegata G Volpato A Romano S Nazari C Tinelli GN Ranzani E Solcia R Fiocca 《Canadian Metallurgical Quarterly》1994,424(4):367-373
Activation of K-ras gene by point mutations, a common finding in lung adenocarcinomas, has been suggested to decrease patient survival. We investigated 109 lung adenocarcinomas, mostly small, peripheral, stage I tumours (81/109) for presence of K-ras gene mutations at codons 12 and 13. Mutations were detected by denaturing gradient gel electrophoresis analysis of specific sequences amplified by polymerase chain reaction from DNA extracted from archival pathological material. Thirty-three of 109 (30.3%) tumours showed mutations at codon 12 (28/33, 84.8%) or 13 (5/33, 15.2%) of the gene. Mutations and type of nucleotide substitutions were differently distributed among cytological subtypes, being more prevalent among less differentiated (G2 and G3) tumours and among bronchial than bronchiolo-alveolar type adenocarcinomas. Survival analysis showed an adverse effect of K-ras mutation on survival, restricted to stage I tumours. Median survival for 81 stage I patients was 30 months for non-mutated tumours versus 20 months for mutated tumours (p = 0.016). Multivariate analysis showed that age of patient (p = 0.001) and K-ras mutation status (p = 0.04) were the only independent factors influencing survival significantly. These data strengthen the hypothesis that K-ras gene mutations may be useful in identifying a subgroup of patients with poor outcome. 相似文献
104.
Kiran R. Chaudhari Abhinesh Kumar Vinoth Kumar Megraj Khandelwal Anil K. Mishra Jukka Monkkonen Rayasa S. Ramachandra Murthy 《Advanced functional materials》2012,22(19):4101-4114
In an attempt to improve tumor localization of docetaxel (DTX)‐loaded nanoparticles (NPs), zoledronic acid (ZOL) is used as a ligand to target bone metastasis. DTX‐loaded ZOL‐conjugated polyethylene glycol (PEG)ylated polybutyl cyanoacrylate (PBCA) NPs are prepared using an anionic polymerization technique. PBCA‐PEG‐ZOL NPs are subjected to cytotoxic assay in both BO2 and MCF‐7 cell lines. Cell cycle arrest and apoptosis induced by the PBCA‐PEG‐ZOL NPs are studied. Quantitative cellular uptake, NP uptake route characterization, confocal microscopy and IPP/ApppI levels are performed. PBCA‐PEG‐ZOL NPs show an enhanced cytotoxic effect in both BO2 as well as MCF‐7 cell lines due to higher uptake following ZOL‐mediated endocytosis. The molecular basis of apoptosis reveals the involvement of a cytoplasmic protein in activating the programmed cell death pathway. Route characterization studies reveal that PBCA‐PEG‐ZOL NPs uptake is not completely blocked even by using both inhibitors (genistein and phenyl arsinoxide) simultaneously, conferring that uptake is not entirely based upon clathrin or caveolae. PBCA‐PEG‐ZOL NPs showed 7 and 5.3 times increase in IPP and ApppI production, in comparison to ZOL treatment, and 138 times higher than the control group in MCF‐7 cell line. In BO2 cell line, after treatment with NPs, IPP was 5.35 times higher than ZOL solution. No ApppI in BO2 cell line after treatment with NPs and ZOL solution was found. NP distribution in tumor infected bone is also significantly high in comparison to the normal bone at any time point. It is concluded that ZOL‐conjugated NPs provide an efficient and targeted delivery of DTX, with synergistic effects. Thus, these NPs present a promising treatment in the near future, by actively targeting metastatic tumor. 相似文献
105.
Characteristics of Mobile Ad hoc Networks such as shared broadcast channel, bandwidth and battery power limitations, highly dynamic topology, and location dependent errors, make provisioning of quality of service (QoS) in such networks very difficult. The Medium Access Control (MAC) layer plays a very important role as far as QoS is concerned. The MAC layer is responsible for selecting the next packet to be transmitted and the timing of its transmission. We have proposed a new MAC layer protocol that includes a laxity-based priority scheduling scheme and an associated back-off scheme, for supporting time-sensitive traffic. In the proposed scheduling scheme, we select the next packet to be transmitted, based on its priority value which takes into consideration the uniform laxity budget of the packet, the current packet delivery ratio of the flow to which the packet belongs, and the packet delivery ratio desired by the user. The back-off mechanism devised by us grants a node access to the channel, based on the rank of its highest priority packet in comparison to other such packets queued at nodes in the neighborhood of the current node. We have studied the performance of our protocol that combines a packet scheduling scheme and a channel access scheme through simulation experiments, and the simulation results show that our protocol exhibits a significant improvement in packet delivery ratio under bounded end-to-end delay requirements, compared to the existing 802.11 DCF and the Distributed Priority Scheduling scheme proposed recently in [ACM Wireless Networks Journal 8 (5) (2002) 455–466; Proceedings of ACM MOBICOM '01, July 2001, pp. 200–209]. 相似文献
106.
107.
P Blok ME Craanen GJ Offerhaus W Dekker EJ Kuipers SG Meuwissen GN Tytgat 《Canadian Metallurgical Quarterly》1999,111(2):241-247
The effect of nitric oxide (NO) donors on high-voltage-activated Ca2+ channels in insulin-secreting RINm5F cells was investigated using the patch-clamp technique in the whole-cell configuration. Sodium nitroprusside (SNP, 2-400 microM) induced a dose-dependent reduction in Ba2+ currents with maximal inhibition of 58%. The IC50 for SNP was 45 microM. A different NO donor, (+/-)S-nitroso-N-acetylpenicillamine (SNAP, 500 microM), also produced a 50% decrease in current amplitude. When 200 microM SNP was administered together with the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidozoline-1-oxyl-3-oxide (carboxy-PTIO, 300 microM), the Ba2+ current inhibition was lowered to 7%. Administration of 500 microM 8-bromoguanosine 3':5'-cyclic monophosphate sodium salt (8-Br-cGMP) mimicked the effects of SNP, causing a comparable decrease (56%) in peak-current amplitude. When soluble guanylyl cyclase was blocked by 10 microM 1H-[1,2, 4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ), the inhibitory effect of 200 microM SNP was reduced from 39% to 15%. The SNP-induced current decrease was 36% of controls after the blockade of L-type Ca2+ channels and 30% in the presence of 2.5 microM omega-conotoxin-MVIIC. These data indicate that NO inhibits both L-type and P/Q-type Ca2+ channels in RINm5F cells, probably by an increase in the intracellular levels of cGMP. NO may then significantly influence the Ca2+-dependent release of hormones from secretory cells as well as that of neurotransmitters from nerve terminals. 相似文献
108.
G. G. Krishna Murthy A. Ghosh S. P. Mehrotra 《Metallurgical and Materials Transactions B》1989,20(1):53-59
A macroscopic, steady state energy balance model has been formulated to describe mixing phenom-ena in a liquid bath stirred
by injecting gas through a straight nozzle fitted axially at the bottom of the vessel. This, along with experimental data
on a water model previously reported, was employed to make predictions. Input energy terms considered in the model consist
of buoyancy energy and empirically determined fraction of gas kinetic energy. Dissipation of energy was attributed to liquid
circulation and bubble slip. The two-phase plume was assumed to be a truncated cone whose dimen-sions depended upon operating
conditions. Numerical solution of model equations gave liquid velocity and gas hold-up inside the plume as well as liquid
circulation rate and liquid velocity in the region outside the plume. Influence of process variables, e.g., gas flow rate,
bath height, and nozzle diameter, have been predicted. Validity of the model has been established by comparing some pre-dicted
entrainment ratios with those experimentally measured by other investigators. Empirical cor-relations to predict circulation
time and circulation number have been proposed. Circulation number was found to vary between 2 and 12 in contrast to the existing
assumption in the literature of a con-stant value of 3. Usefulness of these correlations in predicting mixing time for industrial
vessels has been demonstrated.
Formerly a Graduate Student in the De-partment of Metallurgical Engineering at the Indian Institute of Technol-ogy, Kanpur 相似文献
109.
F.V. Albin S. Srinivasa Murthy M.V. Krishna Murthy 《International Journal of Refrigeration》1979,2(3):129-136
An analysis of the freezing characteristics of spherical shaped food products with time dependent surface-temperature variation is presented. The one dimensional heat conduction equations in spherical coordinates for the frozen and unfrozen regions are solved simultaneously by a suitable transformation to obtain an equivalent slab. Goodman's integral method is applied to obtain the solution in terms of four dimensionless parameters. The results are presented for an exponential variation of the surface temperature for parameter values covering the ranges of the thermophysical properties and processing conditions encountered in food freezing practice. A correlation for facilitating the estimation of the freezing time is also given. 相似文献
110.
Energy-efficient directional routing between partitioned actors in wireless sensor and actor networks 总被引:1,自引:0,他引:1
Actor?actor communication is an important part of the functioning of wireless sensor?actor networks and enables the actor nodes to take coordinated action on a given event. Owing to various reasons such as actor mobility and low actor density, the actor network tends to get partitioned. The authors propose to use the underlying sensor nodes, which are more densely deployed, to heal these partitions. In order to maximise the utilisation of the limited energy available with the sensor nodes, a new routing protocol for actor?actor communication using directional antennas on the actor nodes is proposed. The authors contribution is threefold. First, using simulations they show that the problem of partitioning in the actor networks is significant and propose an architecture with directional antennas on actor nodes and sensor bridges to heal these partitions. Second, they identify the routing problem for this architecture based on a theoretical framework and propose centralised as well as distributed solutions to it. Third, they develop a routing protocol based on the distributed solution and show, using network simulations, that the proposed protocol not only heals the network partitions successfully, but also achieves high throughput and fairness across different flows, in addition to maximising the network lifetime. 相似文献